Experiment: CDM1 without Thiamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14360 and BBR_RS20825 overlap by 29 nucleotides BBR_RS20825 and BBR_RS14365 overlap by 2 nucleotides BBR_RS14365 and BBR_RS14370 are separated by 27 nucleotides
BBR_RS14360: BBR_RS14360 - hypothetical protein, at 995,508 to 996,182
_RS14360
BBR_RS20825: BBR_RS20825 - hypothetical protein, at 996,154 to 996,345
_RS20825
BBR_RS14365: BBR_RS14365 - hypothetical protein, at 996,344 to 997,516
_RS14365
BBR_RS14370: BBR_RS14370 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 997,544 to 998,878
_RS14370
Position (kb) 996
997
998 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 995.375 kb on - strand at 995.439 kb on + strand at 995.484 kb on + strand at 995.492 kb on + strand at 995.556 kb on - strand at 995.558 kb on + strand at 995.599 kb on - strand, within BBR_RS14360 at 995.654 kb on - strand, within BBR_RS14360 at 995.764 kb on + strand, within BBR_RS14360 at 995.764 kb on + strand, within BBR_RS14360 at 995.801 kb on + strand, within BBR_RS14360 at 995.846 kb on + strand, within BBR_RS14360 at 995.854 kb on - strand, within BBR_RS14360 at 995.854 kb on - strand, within BBR_RS14360 at 995.890 kb on + strand, within BBR_RS14360 at 995.981 kb on + strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 996.002 kb on - strand, within BBR_RS14360 at 996.014 kb on + strand, within BBR_RS14360 at 996.023 kb on - strand, within BBR_RS14360 at 996.040 kb on + strand, within BBR_RS14360 at 996.045 kb on + strand, within BBR_RS14360 at 996.066 kb on - strand, within BBR_RS14360 at 996.080 kb on - strand, within BBR_RS14360 at 996.143 kb on - strand at 996.244 kb on + strand, within BBR_RS20825 at 996.244 kb on + strand, within BBR_RS20825 at 996.344 kb on - strand at 996.432 kb on - strand at 996.481 kb on + strand, within BBR_RS14365 at 996.491 kb on - strand, within BBR_RS14365 at 996.507 kb on + strand, within BBR_RS14365 at 996.527 kb on + strand, within BBR_RS14365 at 996.565 kb on + strand, within BBR_RS14365 at 996.578 kb on - strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.730 kb on - strand, within BBR_RS14365 at 996.742 kb on + strand, within BBR_RS14365 at 996.833 kb on + strand, within BBR_RS14365 at 996.848 kb on + strand, within BBR_RS14365 at 996.928 kb on + strand, within BBR_RS14365 at 996.932 kb on - strand, within BBR_RS14365 at 997.011 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.029 kb on - strand, within BBR_RS14365 at 997.143 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.293 kb on + strand, within BBR_RS14365 at 997.344 kb on - strand, within BBR_RS14365 at 997.346 kb on + strand, within BBR_RS14365 at 997.423 kb on + strand at 997.423 kb on + strand at 997.510 kb on + strand at 997.510 kb on + strand at 997.548 kb on - strand at 997.564 kb on + strand at 997.750 kb on + strand, within BBR_RS14370 at 997.777 kb on - strand, within BBR_RS14370 at 997.779 kb on + strand, within BBR_RS14370 at 997.793 kb on - strand, within BBR_RS14370 at 997.853 kb on + strand, within BBR_RS14370 at 997.861 kb on - strand, within BBR_RS14370 at 997.872 kb on + strand, within BBR_RS14370 at 997.911 kb on + strand, within BBR_RS14370 at 997.954 kb on - strand, within BBR_RS14370 at 997.964 kb on - strand, within BBR_RS14370 at 998.056 kb on + strand, within BBR_RS14370 at 998.150 kb on + strand, within BBR_RS14370 at 998.150 kb on + strand, within BBR_RS14370 at 998.186 kb on + strand, within BBR_RS14370 at 998.187 kb on - strand, within BBR_RS14370 at 998.348 kb on + strand, within BBR_RS14370 at 998.358 kb on + strand, within BBR_RS14370 at 998.363 kb on - strand, within BBR_RS14370
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Thiamine remove 995,375 - -3.4 995,439 + +0.5 995,484 + +0.9 995,492 + -0.5 995,556 - -0.7 995,558 + +1.0 995,599 - BBR_RS14360 0.13 -0.2 995,654 - BBR_RS14360 0.22 -1.4 995,764 + BBR_RS14360 0.38 +0.2 995,764 + BBR_RS14360 0.38 -0.7 995,801 + BBR_RS14360 0.43 -0.4 995,846 + BBR_RS14360 0.50 -0.6 995,854 - BBR_RS14360 0.51 -0.7 995,854 - BBR_RS14360 0.51 +0.7 995,890 + BBR_RS14360 0.57 -0.5 995,981 + BBR_RS14360 0.70 -0.3 995,989 - BBR_RS14360 0.71 -2.0 995,989 - BBR_RS14360 0.71 -0.3 995,989 - BBR_RS14360 0.71 -0.5 995,989 - BBR_RS14360 0.71 -1.4 995,989 - BBR_RS14360 0.71 -0.5 995,989 - BBR_RS14360 0.71 +1.4 996,002 - BBR_RS14360 0.73 -0.1 996,014 + BBR_RS14360 0.75 +0.4 996,023 - BBR_RS14360 0.76 -1.1 996,040 + BBR_RS14360 0.79 +0.6 996,045 + BBR_RS14360 0.80 -0.4 996,066 - BBR_RS14360 0.83 -0.1 996,080 - BBR_RS14360 0.85 -1.0 996,143 - -2.4 996,244 + BBR_RS20825 0.47 -0.6 996,244 + BBR_RS20825 0.47 -1.2 996,344 - -2.6 996,432 - -2.6 996,481 + BBR_RS14365 0.12 -3.3 996,491 - BBR_RS14365 0.13 -3.0 996,507 + BBR_RS14365 0.14 -0.3 996,527 + BBR_RS14365 0.16 -1.3 996,565 + BBR_RS14365 0.19 -4.0 996,578 - BBR_RS14365 0.20 -2.9 996,616 + BBR_RS14365 0.23 -3.9 996,616 + BBR_RS14365 0.23 -2.4 996,730 - BBR_RS14365 0.33 -3.7 996,742 + BBR_RS14365 0.34 -4.3 996,833 + BBR_RS14365 0.42 -2.1 996,848 + BBR_RS14365 0.43 -3.9 996,928 + BBR_RS14365 0.50 -1.0 996,932 - BBR_RS14365 0.50 -3.7 997,011 + BBR_RS14365 0.57 -3.4 997,021 + BBR_RS14365 0.58 +0.7 997,021 + BBR_RS14365 0.58 -3.5 997,029 - BBR_RS14365 0.58 -2.9 997,143 - BBR_RS14365 0.68 -2.9 997,150 - BBR_RS14365 0.69 -3.4 997,150 - BBR_RS14365 0.69 -3.1 997,293 + BBR_RS14365 0.81 -1.4 997,344 - BBR_RS14365 0.85 -1.6 997,346 + BBR_RS14365 0.85 -3.8 997,423 + -1.2 997,423 + -2.5 997,510 + -0.8 997,510 + -0.8 997,548 - -1.4 997,564 + +1.7 997,750 + BBR_RS14370 0.15 -1.0 997,777 - BBR_RS14370 0.17 -1.9 997,779 + BBR_RS14370 0.18 -0.3 997,793 - BBR_RS14370 0.19 +1.7 997,853 + BBR_RS14370 0.23 +0.4 997,861 - BBR_RS14370 0.24 -0.1 997,872 + BBR_RS14370 0.25 -0.9 997,911 + BBR_RS14370 0.27 -1.1 997,954 - BBR_RS14370 0.31 -2.0 997,964 - BBR_RS14370 0.31 -0.1 998,056 + BBR_RS14370 0.38 -1.7 998,150 + BBR_RS14370 0.45 -1.2 998,150 + BBR_RS14370 0.45 -0.6 998,186 + BBR_RS14370 0.48 +0.1 998,187 - BBR_RS14370 0.48 +0.7 998,348 + BBR_RS14370 0.60 -2.1 998,358 + BBR_RS14370 0.61 -1.0 998,363 - BBR_RS14370 0.61 -0.1
Or see this region's nucleotide sequence