Experiment: 1600 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.2342.1 and Xcc-8004.2343.1 are separated by 123 nucleotides Xcc-8004.2343.1 and Xcc-8004.2344.1 overlap by 4 nucleotides Xcc-8004.2344.1 and Xcc-8004.2345.1 overlap by 4 nucleotides Xcc-8004.2345.1 and Xcc-8004.2346.1 are separated by 76 nucleotides Xcc-8004.2346.1 and Xcc-8004.2347.1 overlap by 4 nucleotides
Xcc-8004.2342.1: Xcc-8004.2342.1 - Cytochrome c heme lyase subunit CcmF, at 2,289,267 to 2,291,204
Xcc-8004.2342.1
Xcc-8004.2343.1: Xcc-8004.2343.1 - Cytochrome c-type biogenesis protein CcmE, heme chaperone, at 2,291,328 to 2,291,786
Xcc-8004.2343.1
Xcc-8004.2344.1: Xcc-8004.2344.1 - Cytochrome c-type biogenesis protein CcmD, interacts with CcmCE, at 2,291,783 to 2,291,953
Xcc-8004.2344.1
Xcc-8004.2345.1: Xcc-8004.2345.1 - Cytochrome c-type biogenesis protein CcmC, putative heme lyase for CcmE, at 2,291,950 to 2,292,699
Xcc-8004.2345.1
Xcc-8004.2346.1: Xcc-8004.2346.1 - ABC transporter involved in cytochrome c biogenesis, CcmB subunit, at 2,292,776 to 2,293,471
Xcc-8004.2346.1
Xcc-8004.2347.1: Xcc-8004.2347.1 - ABC transporter involved in cytochrome c biogenesis, ATPase component CcmA, at 2,293,468 to 2,294,112
Xcc-8004.2347.1
Position (kb)
2291
2292
2293 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2290.951 kb on + strand, within Xcc-8004.2342.1 at 2290.958 kb on - strand, within Xcc-8004.2342.1 at 2291.223 kb on + strand at 2291.223 kb on + strand at 2291.223 kb on + strand at 2291.224 kb on - strand at 2291.224 kb on - strand at 2291.224 kb on - strand at 2291.224 kb on - strand at 2291.224 kb on - strand at 2291.224 kb on - strand at 2291.246 kb on - strand at 2291.269 kb on - strand at 2291.269 kb on - strand at 2291.269 kb on - strand at 2291.269 kb on - strand at 2291.269 kb on - strand at 2291.269 kb on - strand at 2291.376 kb on - strand, within Xcc-8004.2343.1 at 2291.407 kb on + strand, within Xcc-8004.2343.1 at 2291.407 kb on + strand, within Xcc-8004.2343.1 at 2291.408 kb on - strand, within Xcc-8004.2343.1 at 2291.408 kb on - strand, within Xcc-8004.2343.1 at 2291.675 kb on - strand, within Xcc-8004.2343.1 at 2291.681 kb on - strand, within Xcc-8004.2343.1 at 2291.923 kb on - strand, within Xcc-8004.2344.1 at 2292.518 kb on + strand, within Xcc-8004.2345.1 at 2292.519 kb on - strand, within Xcc-8004.2345.1 at 2292.615 kb on - strand, within Xcc-8004.2345.1 at 2292.721 kb on - strand at 2292.933 kb on + strand, within Xcc-8004.2346.1 at 2292.933 kb on + strand, within Xcc-8004.2346.1 at 2292.934 kb on - strand, within Xcc-8004.2346.1 at 2292.934 kb on - strand, within Xcc-8004.2346.1 at 2292.934 kb on - strand, within Xcc-8004.2346.1 at 2293.521 kb on - strand at 2293.521 kb on - strand at 2293.620 kb on - strand, within Xcc-8004.2347.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1600 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together) remove 2,290,951 + Xcc-8004.2342.1 0.87 +1.7 2,290,958 - Xcc-8004.2342.1 0.87 -0.5 2,291,223 + +0.3 2,291,223 + +0.5 2,291,223 + +0.8 2,291,224 - +0.8 2,291,224 - -1.7 2,291,224 - +0.1 2,291,224 - -0.3 2,291,224 - +0.5 2,291,224 - +1.8 2,291,246 - +1.6 2,291,269 - -0.5 2,291,269 - +0.8 2,291,269 - -0.9 2,291,269 - -2.5 2,291,269 - -0.7 2,291,269 - -1.0 2,291,376 - Xcc-8004.2343.1 0.10 -2.2 2,291,407 + Xcc-8004.2343.1 0.17 -0.9 2,291,407 + Xcc-8004.2343.1 0.17 +0.3 2,291,408 - Xcc-8004.2343.1 0.17 +1.1 2,291,408 - Xcc-8004.2343.1 0.17 -0.5 2,291,675 - Xcc-8004.2343.1 0.76 +0.5 2,291,681 - Xcc-8004.2343.1 0.77 -1.5 2,291,923 - Xcc-8004.2344.1 0.82 +2.5 2,292,518 + Xcc-8004.2345.1 0.76 +0.3 2,292,519 - Xcc-8004.2345.1 0.76 -1.5 2,292,615 - Xcc-8004.2345.1 0.89 -0.9 2,292,721 - +0.1 2,292,933 + Xcc-8004.2346.1 0.23 -1.9 2,292,933 + Xcc-8004.2346.1 0.23 +1.7 2,292,934 - Xcc-8004.2346.1 0.23 +1.7 2,292,934 - Xcc-8004.2346.1 0.23 -0.5 2,292,934 - Xcc-8004.2346.1 0.23 +2.1 2,293,521 - -1.7 2,293,521 - -1.2 2,293,620 - Xcc-8004.2347.1 0.24 +1.5
Or see this region's nucleotide sequence