Experiment: 1600 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.1022.1 and Xcc-8004.1023.1 are separated by 224 nucleotides Xcc-8004.1023.1 and Xcc-8004.1026.1 are separated by 221 nucleotides Xcc-8004.1026.1 and Xcc-8004.1027.1 overlap by 8 nucleotides
Xcc-8004.1022.1: Xcc-8004.1022.1 - hypothetical protein, at 969,026 to 970,354
Xcc-8004.1022.1
Xcc-8004.1023.1: Xcc-8004.1023.1 - Phosphate-specific outer membrane porin OprP ; Pyrophosphate-specific outer membrane porin OprO, at 970,579 to 971,772
Xcc-8004.1023.1
Xcc-8004.1026.1: Xcc-8004.1026.1 - Tricarboxylate transport transcriptional regulator TctD, at 971,994 to 972,740
Xcc-8004.1026.1
Xcc-8004.1027.1: Xcc-8004.1027.1 - Two-component system sensor protein, at 972,733 to 974,118
Xcc-8004.1027.1
Position (kb) 970
971
972 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 969.970 kb on - strand, within Xcc-8004.1022.1 at 970.002 kb on + strand, within Xcc-8004.1022.1 at 970.002 kb on + strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.150 kb on - strand, within Xcc-8004.1022.1 at 970.245 kb on - strand at 970.311 kb on + strand at 970.311 kb on + strand at 970.312 kb on - strand at 970.461 kb on + strand at 970.580 kb on + strand at 970.580 kb on + strand at 970.581 kb on - strand at 970.617 kb on + strand at 970.617 kb on + strand at 970.618 kb on - strand at 970.618 kb on - strand at 970.701 kb on - strand, within Xcc-8004.1023.1 at 970.784 kb on + strand, within Xcc-8004.1023.1 at 970.785 kb on - strand, within Xcc-8004.1023.1 at 970.785 kb on - strand, within Xcc-8004.1023.1 at 970.785 kb on - strand, within Xcc-8004.1023.1 at 970.790 kb on + strand, within Xcc-8004.1023.1 at 970.910 kb on + strand, within Xcc-8004.1023.1 at 970.993 kb on + strand, within Xcc-8004.1023.1 at 970.993 kb on + strand, within Xcc-8004.1023.1 at 970.994 kb on - strand, within Xcc-8004.1023.1 at 971.145 kb on - strand, within Xcc-8004.1023.1 at 971.271 kb on - strand, within Xcc-8004.1023.1 at 971.330 kb on + strand, within Xcc-8004.1023.1 at 971.381 kb on + strand, within Xcc-8004.1023.1 at 971.382 kb on - strand, within Xcc-8004.1023.1 at 971.382 kb on - strand, within Xcc-8004.1023.1 at 971.414 kb on + strand, within Xcc-8004.1023.1 at 971.415 kb on - strand, within Xcc-8004.1023.1 at 971.468 kb on + strand, within Xcc-8004.1023.1 at 971.468 kb on + strand, within Xcc-8004.1023.1 at 971.507 kb on + strand, within Xcc-8004.1023.1 at 971.508 kb on - strand, within Xcc-8004.1023.1 at 971.541 kb on - strand, within Xcc-8004.1023.1 at 971.541 kb on - strand, within Xcc-8004.1023.1 at 971.553 kb on - strand, within Xcc-8004.1023.1 at 971.786 kb on + strand at 971.786 kb on + strand at 971.786 kb on + strand at 971.786 kb on + strand at 971.786 kb on + strand at 971.786 kb on + strand at 971.786 kb on + strand at 971.786 kb on + strand at 971.787 kb on - strand at 971.787 kb on - strand at 971.787 kb on - strand at 971.787 kb on - strand at 971.787 kb on - strand at 972.330 kb on + strand, within Xcc-8004.1026.1 at 972.331 kb on - strand, within Xcc-8004.1026.1 at 972.415 kb on + strand, within Xcc-8004.1026.1 at 972.501 kb on + strand, within Xcc-8004.1026.1 at 972.502 kb on - strand, within Xcc-8004.1026.1 at 972.616 kb on - strand, within Xcc-8004.1026.1 at 972.616 kb on - strand, within Xcc-8004.1026.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1600 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together) remove 969,970 - Xcc-8004.1022.1 0.71 -2.6 970,002 + Xcc-8004.1022.1 0.73 +0.7 970,002 + Xcc-8004.1022.1 0.73 -2.0 970,003 - Xcc-8004.1022.1 0.74 -1.9 970,003 - Xcc-8004.1022.1 0.74 -0.9 970,003 - Xcc-8004.1022.1 0.74 +1.1 970,003 - Xcc-8004.1022.1 0.74 -1.9 970,003 - Xcc-8004.1022.1 0.74 -1.2 970,150 - Xcc-8004.1022.1 0.85 -0.4 970,245 - -1.5 970,311 + -3.3 970,311 + -1.2 970,312 - -3.9 970,461 + -1.2 970,580 + -1.5 970,580 + -2.6 970,581 - -1.2 970,617 + +0.1 970,617 + -1.1 970,618 - -0.2 970,618 - -0.6 970,701 - Xcc-8004.1023.1 0.10 -1.2 970,784 + Xcc-8004.1023.1 0.17 +0.6 970,785 - Xcc-8004.1023.1 0.17 -0.7 970,785 - Xcc-8004.1023.1 0.17 -1.3 970,785 - Xcc-8004.1023.1 0.17 -0.4 970,790 + Xcc-8004.1023.1 0.18 +0.1 970,910 + Xcc-8004.1023.1 0.28 -2.2 970,993 + Xcc-8004.1023.1 0.35 -1.7 970,993 + Xcc-8004.1023.1 0.35 -1.5 970,994 - Xcc-8004.1023.1 0.35 -2.0 971,145 - Xcc-8004.1023.1 0.47 -1.3 971,271 - Xcc-8004.1023.1 0.58 -3.0 971,330 + Xcc-8004.1023.1 0.63 -2.0 971,381 + Xcc-8004.1023.1 0.67 -2.7 971,382 - Xcc-8004.1023.1 0.67 -2.0 971,382 - Xcc-8004.1023.1 0.67 -0.6 971,414 + Xcc-8004.1023.1 0.70 -1.2 971,415 - Xcc-8004.1023.1 0.70 -2.8 971,468 + Xcc-8004.1023.1 0.74 -1.0 971,468 + Xcc-8004.1023.1 0.74 -1.1 971,507 + Xcc-8004.1023.1 0.78 -0.7 971,508 - Xcc-8004.1023.1 0.78 +0.5 971,541 - Xcc-8004.1023.1 0.81 -2.5 971,541 - Xcc-8004.1023.1 0.81 -0.9 971,553 - Xcc-8004.1023.1 0.82 -1.1 971,786 + +0.1 971,786 + -2.0 971,786 + -2.1 971,786 + -1.7 971,786 + -0.7 971,786 + -2.7 971,786 + -0.7 971,786 + -2.0 971,787 - -1.4 971,787 - +0.1 971,787 - -2.0 971,787 - -1.7 971,787 - -0.0 972,330 + Xcc-8004.1026.1 0.45 -1.4 972,331 - Xcc-8004.1026.1 0.45 -0.7 972,415 + Xcc-8004.1026.1 0.56 -1.3 972,501 + Xcc-8004.1026.1 0.68 -1.5 972,502 - Xcc-8004.1026.1 0.68 -1.7 972,616 - Xcc-8004.1026.1 0.83 -2.1 972,616 - Xcc-8004.1026.1 0.83 +0.1
Or see this region's nucleotide sequence