Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25975 and RR42_RS25980 are separated by 220 nucleotides RR42_RS25980 and RR42_RS25985 are separated by 90 nucleotides RR42_RS25985 and RR42_RS25990 overlap by 4 nucleotides RR42_RS25990 and RR42_RS25995 are separated by 3 nucleotides
RR42_RS25975: RR42_RS25975 - hypothetical protein, at 1,172,956 to 1,173,195
_RS25975
RR42_RS25980: RR42_RS25980 - hypothetical protein, at 1,173,416 to 1,174,057
_RS25980
RR42_RS25985: RR42_RS25985 - cytochrome, at 1,174,148 to 1,174,522
_RS25985
RR42_RS25990: RR42_RS25990 - cytochrome o ubiquinol oxidase subunit III, at 1,174,519 to 1,175,121
_RS25990
RR42_RS25995: RR42_RS25995 - cytochrome o ubiquinol oxidase subunit I, at 1,175,125 to 1,177,101
_RS25995
Position (kb) 1174
1175 Strain fitness (log2 ratio) -1
0
1
2 at 1173.263 kb on + strand at 1173.268 kb on + strand at 1173.269 kb on - strand at 1173.362 kb on + strand at 1173.362 kb on + strand at 1173.363 kb on - strand at 1173.363 kb on - strand at 1173.369 kb on + strand at 1173.369 kb on + strand at 1173.370 kb on - strand at 1173.437 kb on + strand at 1173.528 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.912 kb on - strand at 1173.912 kb on - strand, within RR42_RS25980 at 1173.912 kb on - strand, within RR42_RS25980 at 1173.947 kb on + strand, within RR42_RS25980 at 1173.947 kb on + strand, within RR42_RS25980 at 1174.149 kb on + strand at 1174.149 kb on + strand at 1174.149 kb on + strand at 1174.150 kb on - strand at 1174.150 kb on - strand at 1174.181 kb on - strand at 1174.522 kb on + strand at 1174.523 kb on - strand at 1174.523 kb on - strand at 1174.539 kb on - strand at 1174.718 kb on + strand, within RR42_RS25990 at 1174.718 kb on + strand, within RR42_RS25990 at 1174.719 kb on - strand, within RR42_RS25990 at 1174.782 kb on - strand, within RR42_RS25990 at 1174.782 kb on - strand, within RR42_RS25990 at 1174.782 kb on - strand, within RR42_RS25990 at 1174.782 kb on - strand, within RR42_RS25990 at 1174.782 kb on - strand, within RR42_RS25990 at 1175.040 kb on - strand, within RR42_RS25990 at 1175.098 kb on - strand at 1175.115 kb on - strand at 1175.140 kb on + strand at 1175.141 kb on - strand at 1175.192 kb on + strand at 1175.192 kb on + strand at 1175.193 kb on - strand at 1175.193 kb on - strand at 1175.196 kb on - strand at 1175.196 kb on - strand at 1175.213 kb on + strand at 1175.349 kb on + strand, within RR42_RS25995 at 1175.453 kb on + strand, within RR42_RS25995 at 1175.453 kb on + strand, within RR42_RS25995 at 1175.454 kb on - strand, within RR42_RS25995
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,173,263 + +0.6 1,173,268 + +0.1 1,173,269 - -0.0 1,173,362 + +0.7 1,173,362 + -0.3 1,173,363 - +0.0 1,173,363 - +1.0 1,173,369 + +0.1 1,173,369 + +0.9 1,173,370 - -0.0 1,173,437 + +0.7 1,173,528 + RR42_RS25980 0.17 -0.4 1,173,655 + RR42_RS25980 0.37 +0.0 1,173,655 + RR42_RS25980 0.37 -0.4 1,173,655 + RR42_RS25980 0.37 -1.0 1,173,655 + RR42_RS25980 0.37 -0.5 1,173,912 - +1.9 1,173,912 - RR42_RS25980 0.77 -0.3 1,173,912 - RR42_RS25980 0.77 -0.9 1,173,947 + RR42_RS25980 0.83 -1.2 1,173,947 + RR42_RS25980 0.83 +0.2 1,174,149 + +0.6 1,174,149 + +0.8 1,174,149 + +0.5 1,174,150 - +0.5 1,174,150 - +1.1 1,174,181 - +0.0 1,174,522 + +0.7 1,174,523 - +0.1 1,174,523 - +0.5 1,174,539 - -1.0 1,174,718 + RR42_RS25990 0.33 +0.9 1,174,718 + RR42_RS25990 0.33 +0.9 1,174,719 - RR42_RS25990 0.33 +0.7 1,174,782 - RR42_RS25990 0.44 +0.1 1,174,782 - RR42_RS25990 0.44 +0.6 1,174,782 - RR42_RS25990 0.44 +0.5 1,174,782 - RR42_RS25990 0.44 -0.1 1,174,782 - RR42_RS25990 0.44 +0.4 1,175,040 - RR42_RS25990 0.86 -0.1 1,175,098 - +0.4 1,175,115 - +0.1 1,175,140 + +0.1 1,175,141 - -0.9 1,175,192 + +0.0 1,175,192 + +0.0 1,175,193 - -1.3 1,175,193 - -0.3 1,175,196 - -0.7 1,175,196 - +0.6 1,175,213 + -0.0 1,175,349 + RR42_RS25995 0.11 +0.3 1,175,453 + RR42_RS25995 0.17 -0.0 1,175,453 + RR42_RS25995 0.17 -0.1 1,175,454 - RR42_RS25995 0.17 +0.6
Or see this region's nucleotide sequence