Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21785 and RR42_RS21790 are separated by 58 nucleotides RR42_RS21790 and RR42_RS21795 are separated by 62 nucleotides RR42_RS21795 and RR42_RS21800 overlap by 4 nucleotides
RR42_RS21785: RR42_RS21785 - MFS transporter, at 215,941 to 218,130
_RS21785
RR42_RS21790: RR42_RS21790 - short-chain dehydrogenase, at 218,189 to 218,947
_RS21790
RR42_RS21795: RR42_RS21795 - mannosyltransferase, at 219,010 to 219,744
_RS21795
RR42_RS21800: RR42_RS21800 - branched-chain amino acid ABC transporter substrate-binding protein, at 219,741 to 220,499
_RS21800
Position (kb) 218
219 Strain fitness (log2 ratio) -1
0
1 at 217.290 kb on - strand, within RR42_RS21785 at 217.361 kb on + strand, within RR42_RS21785 at 217.361 kb on + strand, within RR42_RS21785 at 217.405 kb on - strand, within RR42_RS21785 at 217.577 kb on + strand, within RR42_RS21785 at 218.119 kb on - strand at 218.392 kb on - strand, within RR42_RS21790 at 218.425 kb on - strand, within RR42_RS21790 at 218.487 kb on + strand, within RR42_RS21790 at 218.996 kb on - strand at 219.013 kb on + strand at 219.013 kb on + strand at 219.014 kb on - strand at 219.042 kb on + strand at 219.116 kb on - strand, within RR42_RS21795 at 219.356 kb on + strand, within RR42_RS21795 at 219.357 kb on - strand, within RR42_RS21795 at 219.473 kb on + strand, within RR42_RS21795 at 219.500 kb on + strand, within RR42_RS21795 at 219.501 kb on - strand, within RR42_RS21795 at 219.501 kb on - strand, within RR42_RS21795 at 219.532 kb on + strand, within RR42_RS21795 at 219.533 kb on - strand, within RR42_RS21795 at 219.622 kb on + strand, within RR42_RS21795 at 219.622 kb on + strand, within RR42_RS21795 at 219.623 kb on - strand, within RR42_RS21795 at 219.727 kb on + strand at 219.766 kb on + strand at 219.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 217,290 - RR42_RS21785 0.62 +0.2 217,361 + RR42_RS21785 0.65 -1.5 217,361 + RR42_RS21785 0.65 -0.3 217,405 - RR42_RS21785 0.67 -0.7 217,577 + RR42_RS21785 0.75 +0.5 218,119 - +0.7 218,392 - RR42_RS21790 0.27 +0.4 218,425 - RR42_RS21790 0.31 -0.0 218,487 + RR42_RS21790 0.39 -1.2 218,996 - +0.8 219,013 + -0.6 219,013 + +0.6 219,014 - +0.1 219,042 + -0.3 219,116 - RR42_RS21795 0.14 +1.2 219,356 + RR42_RS21795 0.47 -0.8 219,357 - RR42_RS21795 0.47 +0.5 219,473 + RR42_RS21795 0.63 -0.8 219,500 + RR42_RS21795 0.67 -0.6 219,501 - RR42_RS21795 0.67 -0.8 219,501 - RR42_RS21795 0.67 +0.2 219,532 + RR42_RS21795 0.71 +0.3 219,533 - RR42_RS21795 0.71 +1.2 219,622 + RR42_RS21795 0.83 -0.4 219,622 + RR42_RS21795 0.83 +0.2 219,623 - RR42_RS21795 0.83 -0.5 219,727 + +0.3 219,766 + -1.0 219,767 - +0.5
Or see this region's nucleotide sequence