Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10650 and RR42_RS10655 overlap by 4 nucleotides RR42_RS10655 and RR42_RS10660 overlap by 4 nucleotides
RR42_RS10650: RR42_RS10650 - acetyl-CoA synthetase, at 2,274,996 to 2,276,762
_RS10650
RR42_RS10655: RR42_RS10655 - pyruvate dehydrogenase, at 2,276,759 to 2,277,781
_RS10655
RR42_RS10660: RR42_RS10660 - pyruvate dehydrogenase, at 2,277,778 to 2,278,749
_RS10660
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.782 kb on - strand, within RR42_RS10650 at 2275.815 kb on + strand, within RR42_RS10650 at 2275.890 kb on + strand, within RR42_RS10650 at 2275.926 kb on + strand, within RR42_RS10650 at 2275.926 kb on + strand, within RR42_RS10650 at 2275.986 kb on + strand, within RR42_RS10650 at 2276.228 kb on - strand, within RR42_RS10650 at 2276.228 kb on - strand, within RR42_RS10650 at 2276.230 kb on + strand, within RR42_RS10650 at 2276.231 kb on - strand, within RR42_RS10650 at 2276.231 kb on - strand, within RR42_RS10650 at 2276.382 kb on + strand, within RR42_RS10650 at 2276.383 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.518 kb on + strand, within RR42_RS10650 at 2276.519 kb on - strand, within RR42_RS10650 at 2276.736 kb on - strand at 2276.816 kb on - strand at 2276.889 kb on - strand, within RR42_RS10655 at 2276.904 kb on + strand, within RR42_RS10655 at 2276.905 kb on - strand, within RR42_RS10655 at 2276.924 kb on + strand, within RR42_RS10655 at 2276.924 kb on + strand, within RR42_RS10655 at 2276.925 kb on - strand, within RR42_RS10655 at 2276.996 kb on + strand, within RR42_RS10655 at 2276.997 kb on - strand, within RR42_RS10655 at 2277.059 kb on + strand, within RR42_RS10655 at 2277.395 kb on + strand, within RR42_RS10655 at 2277.396 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.515 kb on + strand, within RR42_RS10655 at 2277.516 kb on - strand, within RR42_RS10655 at 2277.540 kb on - strand, within RR42_RS10655 at 2277.554 kb on + strand, within RR42_RS10655 at 2277.604 kb on + strand, within RR42_RS10655 at 2277.605 kb on - strand, within RR42_RS10655 at 2277.605 kb on - strand, within RR42_RS10655 at 2277.755 kb on + strand at 2277.889 kb on + strand, within RR42_RS10660 at 2277.936 kb on - strand, within RR42_RS10660 at 2278.062 kb on - strand, within RR42_RS10660 at 2278.078 kb on + strand, within RR42_RS10660 at 2278.086 kb on + strand, within RR42_RS10660 at 2278.118 kb on + strand, within RR42_RS10660 at 2278.122 kb on - strand, within RR42_RS10660 at 2278.186 kb on + strand, within RR42_RS10660 at 2278.187 kb on - strand, within RR42_RS10660 at 2278.245 kb on + strand, within RR42_RS10660 at 2278.246 kb on - strand, within RR42_RS10660 at 2278.307 kb on - strand, within RR42_RS10660 at 2278.307 kb on - strand, within RR42_RS10660 at 2278.326 kb on - strand, within RR42_RS10660 at 2278.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,275,773 + RR42_RS10650 0.44 -0.2 2,275,773 + RR42_RS10650 0.44 -0.2 2,275,773 + RR42_RS10650 0.44 -0.6 2,275,782 - RR42_RS10650 0.44 -0.1 2,275,815 + RR42_RS10650 0.46 -1.7 2,275,890 + RR42_RS10650 0.51 +0.5 2,275,926 + RR42_RS10650 0.53 +1.1 2,275,926 + RR42_RS10650 0.53 +0.1 2,275,986 + RR42_RS10650 0.56 +1.7 2,276,228 - RR42_RS10650 0.70 -0.0 2,276,228 - RR42_RS10650 0.70 +0.3 2,276,230 + RR42_RS10650 0.70 -0.1 2,276,231 - RR42_RS10650 0.70 +0.1 2,276,231 - RR42_RS10650 0.70 -0.0 2,276,382 + RR42_RS10650 0.78 -0.0 2,276,383 - RR42_RS10650 0.78 +2.0 2,276,386 - RR42_RS10650 0.79 +0.4 2,276,386 - RR42_RS10650 0.79 -1.2 2,276,386 - RR42_RS10650 0.79 +0.1 2,276,518 + RR42_RS10650 0.86 -0.1 2,276,519 - RR42_RS10650 0.86 +0.7 2,276,736 - +0.7 2,276,816 - +0.0 2,276,889 - RR42_RS10655 0.13 +0.1 2,276,904 + RR42_RS10655 0.14 +0.3 2,276,905 - RR42_RS10655 0.14 +0.6 2,276,924 + RR42_RS10655 0.16 -0.1 2,276,924 + RR42_RS10655 0.16 +0.5 2,276,925 - RR42_RS10655 0.16 -0.1 2,276,996 + RR42_RS10655 0.23 +0.7 2,276,997 - RR42_RS10655 0.23 -0.6 2,277,059 + RR42_RS10655 0.29 +0.2 2,277,395 + RR42_RS10655 0.62 +0.2 2,277,396 - RR42_RS10655 0.62 +0.0 2,277,401 - RR42_RS10655 0.63 +1.4 2,277,401 - RR42_RS10655 0.63 +0.2 2,277,401 - RR42_RS10655 0.63 +0.8 2,277,515 + RR42_RS10655 0.74 -1.5 2,277,516 - RR42_RS10655 0.74 -0.1 2,277,540 - RR42_RS10655 0.76 -0.3 2,277,554 + RR42_RS10655 0.78 +0.2 2,277,604 + RR42_RS10655 0.83 +0.1 2,277,605 - RR42_RS10655 0.83 +0.4 2,277,605 - RR42_RS10655 0.83 +1.7 2,277,755 + +0.3 2,277,889 + RR42_RS10660 0.11 +1.1 2,277,936 - RR42_RS10660 0.16 -1.1 2,278,062 - RR42_RS10660 0.29 +0.5 2,278,078 + RR42_RS10660 0.31 +0.5 2,278,086 + RR42_RS10660 0.32 +0.0 2,278,118 + RR42_RS10660 0.35 +0.1 2,278,122 - RR42_RS10660 0.35 -0.2 2,278,186 + RR42_RS10660 0.42 +0.2 2,278,187 - RR42_RS10660 0.42 -1.7 2,278,245 + RR42_RS10660 0.48 +2.0 2,278,246 - RR42_RS10660 0.48 +0.4 2,278,307 - RR42_RS10660 0.54 -3.0 2,278,307 - RR42_RS10660 0.54 -0.9 2,278,326 - RR42_RS10660 0.56 -0.5 2,278,673 - -0.6
Or see this region's nucleotide sequence