Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00650 and RR42_RS00655 are separated by 125 nucleotides RR42_RS00655 and RR42_RS00660 are separated by 3 nucleotides RR42_RS00660 and RR42_RS00665 are separated by 70 nucleotides RR42_RS00665 and RR42_RS00670 are separated by 116 nucleotides
RR42_RS00650: RR42_RS00650 - hypothetical protein, at 168,347 to 169,087
_RS00650
RR42_RS00655: RR42_RS00655 - membrane protein, at 169,213 to 169,950
_RS00655
RR42_RS00660: RR42_RS00660 - permease, at 169,954 to 170,904
_RS00660
RR42_RS00665: RR42_RS00665 - pyrrolidone-carboxylate peptidase, at 170,975 to 171,649
_RS00665
RR42_RS00670: RR42_RS00670 - teicoplanin resistance protein VanZ, at 171,766 to 172,950
_RS00670
Position (kb)
169
170
171 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.969 kb on + strand, within RR42_RS00650 at 169.103 kb on + strand at 169.104 kb on - strand at 169.104 kb on - strand at 169.199 kb on + strand at 169.199 kb on + strand at 169.200 kb on - strand at 169.200 kb on - strand at 169.241 kb on + strand at 169.241 kb on + strand at 169.242 kb on - strand at 169.523 kb on - strand, within RR42_RS00655 at 169.599 kb on - strand, within RR42_RS00655 at 169.695 kb on + strand, within RR42_RS00655 at 169.948 kb on + strand at 169.972 kb on + strand at 169.972 kb on + strand at 169.972 kb on + strand at 169.979 kb on - strand at 169.979 kb on - strand at 170.077 kb on + strand, within RR42_RS00660 at 170.077 kb on + strand, within RR42_RS00660 at 170.557 kb on + strand, within RR42_RS00660 at 170.557 kb on + strand, within RR42_RS00660 at 170.557 kb on + strand, within RR42_RS00660 at 170.558 kb on - strand, within RR42_RS00660 at 170.558 kb on - strand, within RR42_RS00660 at 170.559 kb on + strand, within RR42_RS00660 at 170.559 kb on + strand, within RR42_RS00660 at 170.560 kb on - strand, within RR42_RS00660 at 170.573 kb on - strand, within RR42_RS00660 at 170.596 kb on + strand, within RR42_RS00660 at 170.596 kb on + strand, within RR42_RS00660 at 170.596 kb on + strand, within RR42_RS00660 at 170.597 kb on - strand, within RR42_RS00660 at 170.619 kb on + strand, within RR42_RS00660 at 170.878 kb on - strand at 170.878 kb on - strand at 170.890 kb on + strand at 170.890 kb on + strand at 170.903 kb on - strand at 170.903 kb on - strand at 170.903 kb on - strand at 171.329 kb on + strand, within RR42_RS00665 at 171.329 kb on + strand, within RR42_RS00665 at 171.437 kb on + strand, within RR42_RS00665 at 171.438 kb on - strand, within RR42_RS00665 at 171.438 kb on - strand, within RR42_RS00665 at 171.502 kb on + strand, within RR42_RS00665 at 171.510 kb on - strand, within RR42_RS00665 at 171.822 kb on - strand at 171.822 kb on - strand at 171.822 kb on - strand at 171.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 168,965 + RR42_RS00650 0.83 +1.7 168,965 + RR42_RS00650 0.83 -1.3 168,965 + RR42_RS00650 0.83 -0.9 168,965 + RR42_RS00650 0.83 +0.3 168,965 + RR42_RS00650 0.83 +0.1 168,965 + RR42_RS00650 0.83 -0.2 168,966 - RR42_RS00650 0.84 -0.5 168,966 - RR42_RS00650 0.84 -0.8 168,966 - RR42_RS00650 0.84 +2.9 168,966 - RR42_RS00650 0.84 -0.4 168,966 - RR42_RS00650 0.84 +0.1 168,966 - RR42_RS00650 0.84 +0.2 168,966 - RR42_RS00650 0.84 -1.7 168,966 - RR42_RS00650 0.84 +0.5 168,966 - RR42_RS00650 0.84 -1.1 168,969 + RR42_RS00650 0.84 -1.1 169,103 + -0.1 169,104 - -0.1 169,104 - -0.5 169,199 + -0.2 169,199 + +1.3 169,200 - -1.5 169,200 - -0.7 169,241 + -1.1 169,241 + -0.7 169,242 - -0.3 169,523 - RR42_RS00655 0.42 -0.6 169,599 - RR42_RS00655 0.52 -0.2 169,695 + RR42_RS00655 0.65 +2.0 169,948 + -0.1 169,972 + -0.3 169,972 + -0.7 169,972 + -0.2 169,979 - -1.2 169,979 - -0.8 170,077 + RR42_RS00660 0.13 -1.5 170,077 + RR42_RS00660 0.13 -1.1 170,557 + RR42_RS00660 0.63 -1.6 170,557 + RR42_RS00660 0.63 +0.7 170,557 + RR42_RS00660 0.63 -0.3 170,558 - RR42_RS00660 0.64 -0.8 170,558 - RR42_RS00660 0.64 -0.7 170,559 + RR42_RS00660 0.64 +0.1 170,559 + RR42_RS00660 0.64 -0.7 170,560 - RR42_RS00660 0.64 -0.6 170,573 - RR42_RS00660 0.65 +0.1 170,596 + RR42_RS00660 0.68 -2.3 170,596 + RR42_RS00660 0.68 -0.8 170,596 + RR42_RS00660 0.68 -1.4 170,597 - RR42_RS00660 0.68 -1.1 170,619 + RR42_RS00660 0.70 -1.1 170,878 - -0.3 170,878 - +0.2 170,890 + -1.4 170,890 + +0.0 170,903 - -0.5 170,903 - -1.6 170,903 - -1.6 171,329 + RR42_RS00665 0.52 -0.6 171,329 + RR42_RS00665 0.52 +0.2 171,437 + RR42_RS00665 0.68 -0.4 171,438 - RR42_RS00665 0.69 +1.1 171,438 - RR42_RS00665 0.69 +1.3 171,502 + RR42_RS00665 0.78 +0.2 171,510 - RR42_RS00665 0.79 +1.4 171,822 - +0.6 171,822 - -0.4 171,822 - +0.4 171,822 - +0.5
Or see this region's nucleotide sequence