Experiment: CDM1 without Phenylalanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16275 and BBR_RS16280 are separated by 42 nucleotides BBR_RS16280 and BBR_RS16285 are separated by 12 nucleotides BBR_RS16285 and BBR_RS16290 are separated by 179 nucleotides
BBR_RS16275: BBR_RS16275 - leucine--tRNA ligase, at 1,397,248 to 1,400,211
_RS16275
BBR_RS16280: BBR_RS16280 - solute binding protein of ABC transporter system, at 1,400,254 to 1,401,135
_RS16280
BBR_RS16285: BBR_RS16285 - magnesium transporter CorA family protein, at 1,401,148 to 1,402,098
_RS16285
BBR_RS16290: BBR_RS16290 - histidine phosphatase family protein, at 1,402,278 to 1,403,123
_RS16290
Position (kb)
1401
1402
1403 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1400.297 kb on - strand at 1400.328 kb on - strand at 1400.328 kb on - strand at 1400.343 kb on + strand, within BBR_RS16280 at 1400.408 kb on - strand, within BBR_RS16280 at 1400.457 kb on - strand, within BBR_RS16280 at 1400.459 kb on + strand, within BBR_RS16280 at 1400.459 kb on + strand, within BBR_RS16280 at 1400.467 kb on - strand, within BBR_RS16280 at 1400.474 kb on + strand, within BBR_RS16280 at 1400.474 kb on + strand, within BBR_RS16280 at 1400.474 kb on + strand, within BBR_RS16280 at 1400.494 kb on + strand, within BBR_RS16280 at 1400.494 kb on + strand, within BBR_RS16280 at 1400.516 kb on - strand, within BBR_RS16280 at 1400.534 kb on - strand, within BBR_RS16280 at 1400.534 kb on - strand, within BBR_RS16280 at 1400.564 kb on + strand, within BBR_RS16280 at 1400.572 kb on - strand, within BBR_RS16280 at 1400.595 kb on - strand, within BBR_RS16280 at 1400.603 kb on + strand, within BBR_RS16280 at 1400.641 kb on + strand, within BBR_RS16280 at 1400.647 kb on - strand, within BBR_RS16280 at 1400.652 kb on - strand, within BBR_RS16280 at 1400.703 kb on + strand, within BBR_RS16280 at 1400.708 kb on + strand, within BBR_RS16280 at 1400.708 kb on + strand, within BBR_RS16280 at 1400.711 kb on - strand, within BBR_RS16280 at 1400.711 kb on - strand, within BBR_RS16280 at 1400.719 kb on - strand, within BBR_RS16280 at 1400.721 kb on - strand, within BBR_RS16280 at 1400.722 kb on + strand, within BBR_RS16280 at 1400.730 kb on - strand, within BBR_RS16280 at 1400.730 kb on - strand, within BBR_RS16280 at 1400.793 kb on - strand, within BBR_RS16280 at 1400.809 kb on - strand, within BBR_RS16280 at 1400.811 kb on - strand, within BBR_RS16280 at 1400.846 kb on + strand, within BBR_RS16280 at 1400.873 kb on - strand, within BBR_RS16280 at 1400.901 kb on + strand, within BBR_RS16280 at 1400.976 kb on - strand, within BBR_RS16280 at 1400.991 kb on + strand, within BBR_RS16280 at 1401.026 kb on + strand, within BBR_RS16280 at 1401.041 kb on + strand, within BBR_RS16280 at 1401.041 kb on + strand, within BBR_RS16280 at 1401.041 kb on + strand, within BBR_RS16280 at 1401.041 kb on + strand, within BBR_RS16280 at 1401.049 kb on - strand at 1401.049 kb on - strand at 1401.049 kb on - strand at 1401.049 kb on - strand at 1401.049 kb on - strand at 1401.064 kb on + strand at 1401.072 kb on - strand at 1401.072 kb on - strand at 1401.072 kb on - strand at 1401.072 kb on - strand at 1401.072 kb on - strand at 1401.082 kb on - strand at 1401.082 kb on - strand at 1401.105 kb on - strand at 1401.169 kb on + strand at 1401.178 kb on - strand at 1401.245 kb on - strand, within BBR_RS16285 at 1401.245 kb on - strand, within BBR_RS16285 at 1401.259 kb on + strand, within BBR_RS16285 at 1401.368 kb on - strand, within BBR_RS16285 at 1401.391 kb on + strand, within BBR_RS16285 at 1401.410 kb on - strand, within BBR_RS16285 at 1401.492 kb on + strand, within BBR_RS16285 at 1401.561 kb on + strand, within BBR_RS16285 at 1401.614 kb on - strand, within BBR_RS16285 at 1401.706 kb on - strand, within BBR_RS16285 at 1401.731 kb on + strand, within BBR_RS16285 at 1401.776 kb on + strand, within BBR_RS16285 at 1401.776 kb on + strand, within BBR_RS16285 at 1401.779 kb on - strand, within BBR_RS16285 at 1401.779 kb on - strand, within BBR_RS16285 at 1401.875 kb on + strand, within BBR_RS16285 at 1401.940 kb on + strand, within BBR_RS16285 at 1401.999 kb on + strand, within BBR_RS16285 at 1402.029 kb on + strand at 1402.037 kb on - strand at 1402.037 kb on - strand at 1402.121 kb on + strand at 1402.197 kb on + strand at 1402.216 kb on - strand at 1402.338 kb on + strand at 1402.338 kb on + strand at 1402.338 kb on - strand at 1402.436 kb on + strand, within BBR_RS16290 at 1402.444 kb on - strand, within BBR_RS16290 at 1402.476 kb on - strand, within BBR_RS16290 at 1402.477 kb on - strand, within BBR_RS16290 at 1402.511 kb on - strand, within BBR_RS16290 at 1402.535 kb on + strand, within BBR_RS16290 at 1402.543 kb on - strand, within BBR_RS16290 at 1402.543 kb on - strand, within BBR_RS16290 at 1402.550 kb on + strand, within BBR_RS16290 at 1402.555 kb on + strand, within BBR_RS16290 at 1402.558 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.690 kb on + strand, within BBR_RS16290 at 1402.690 kb on + strand, within BBR_RS16290 at 1402.690 kb on + strand, within BBR_RS16290 at 1402.690 kb on + strand, within BBR_RS16290 at 1402.698 kb on - strand, within BBR_RS16290 at 1402.711 kb on + strand, within BBR_RS16290 at 1402.711 kb on + strand, within BBR_RS16290 at 1402.711 kb on + strand, within BBR_RS16290 at 1402.719 kb on - strand, within BBR_RS16290 at 1402.719 kb on - strand, within BBR_RS16290 at 1402.728 kb on + strand, within BBR_RS16290 at 1402.728 kb on + strand, within BBR_RS16290 at 1402.728 kb on + strand, within BBR_RS16290 at 1402.728 kb on + strand, within BBR_RS16290 at 1402.736 kb on - strand, within BBR_RS16290 at 1402.785 kb on + strand, within BBR_RS16290 at 1402.791 kb on + strand, within BBR_RS16290 at 1402.817 kb on - strand, within BBR_RS16290 at 1402.853 kb on - strand, within BBR_RS16290 at 1402.853 kb on - strand, within BBR_RS16290 at 1402.900 kb on - strand, within BBR_RS16290 at 1402.921 kb on + strand, within BBR_RS16290 at 1402.929 kb on - strand, within BBR_RS16290 at 1402.990 kb on - strand, within BBR_RS16290 at 1403.019 kb on + strand, within BBR_RS16290 at 1403.032 kb on - strand, within BBR_RS16290
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Phenylalanine remove 1,400,297 - +1.0 1,400,328 - -0.6 1,400,328 - +1.3 1,400,343 + BBR_RS16280 0.10 -0.1 1,400,408 - BBR_RS16280 0.17 +1.5 1,400,457 - BBR_RS16280 0.23 +0.4 1,400,459 + BBR_RS16280 0.23 +1.2 1,400,459 + BBR_RS16280 0.23 -0.2 1,400,467 - BBR_RS16280 0.24 +1.9 1,400,474 + BBR_RS16280 0.25 +0.7 1,400,474 + BBR_RS16280 0.25 -0.5 1,400,474 + BBR_RS16280 0.25 -0.4 1,400,494 + BBR_RS16280 0.27 -1.3 1,400,494 + BBR_RS16280 0.27 +1.3 1,400,516 - BBR_RS16280 0.30 +0.3 1,400,534 - BBR_RS16280 0.32 -0.6 1,400,534 - BBR_RS16280 0.32 +2.4 1,400,564 + BBR_RS16280 0.35 +0.4 1,400,572 - BBR_RS16280 0.36 +1.2 1,400,595 - BBR_RS16280 0.39 +1.6 1,400,603 + BBR_RS16280 0.40 +1.0 1,400,641 + BBR_RS16280 0.44 +2.4 1,400,647 - BBR_RS16280 0.45 -0.0 1,400,652 - BBR_RS16280 0.45 -1.8 1,400,703 + BBR_RS16280 0.51 +1.5 1,400,708 + BBR_RS16280 0.51 -1.1 1,400,708 + BBR_RS16280 0.51 +1.3 1,400,711 - BBR_RS16280 0.52 +2.3 1,400,711 - BBR_RS16280 0.52 +0.9 1,400,719 - BBR_RS16280 0.53 +0.2 1,400,721 - BBR_RS16280 0.53 -0.4 1,400,722 + BBR_RS16280 0.53 +2.9 1,400,730 - BBR_RS16280 0.54 +2.1 1,400,730 - BBR_RS16280 0.54 +1.5 1,400,793 - BBR_RS16280 0.61 +2.2 1,400,809 - BBR_RS16280 0.63 +2.2 1,400,811 - BBR_RS16280 0.63 +2.3 1,400,846 + BBR_RS16280 0.67 -1.1 1,400,873 - BBR_RS16280 0.70 -0.3 1,400,901 + BBR_RS16280 0.73 -1.3 1,400,976 - BBR_RS16280 0.82 -1.1 1,400,991 + BBR_RS16280 0.84 -2.2 1,401,026 + BBR_RS16280 0.88 +0.9 1,401,041 + BBR_RS16280 0.89 -1.5 1,401,041 + BBR_RS16280 0.89 -2.1 1,401,041 + BBR_RS16280 0.89 +2.5 1,401,041 + BBR_RS16280 0.89 +1.4 1,401,049 - -1.5 1,401,049 - -1.5 1,401,049 - -0.9 1,401,049 - -0.4 1,401,049 - +2.6 1,401,064 + +1.8 1,401,072 - -0.2 1,401,072 - +1.1 1,401,072 - +0.2 1,401,072 - +1.7 1,401,072 - +1.8 1,401,082 - -0.1 1,401,082 - +2.0 1,401,105 - -1.8 1,401,169 + +3.7 1,401,178 - +4.1 1,401,245 - BBR_RS16285 0.10 +4.8 1,401,245 - BBR_RS16285 0.10 -0.3 1,401,259 + BBR_RS16285 0.12 +3.7 1,401,368 - BBR_RS16285 0.23 +4.0 1,401,391 + BBR_RS16285 0.26 +5.9 1,401,410 - BBR_RS16285 0.28 +2.1 1,401,492 + BBR_RS16285 0.36 +1.0 1,401,561 + BBR_RS16285 0.43 +4.9 1,401,614 - BBR_RS16285 0.49 +0.7 1,401,706 - BBR_RS16285 0.59 +3.1 1,401,731 + BBR_RS16285 0.61 +4.9 1,401,776 + BBR_RS16285 0.66 +2.6 1,401,776 + BBR_RS16285 0.66 +4.1 1,401,779 - BBR_RS16285 0.66 -0.1 1,401,779 - BBR_RS16285 0.66 +3.6 1,401,875 + BBR_RS16285 0.76 +1.9 1,401,940 + BBR_RS16285 0.83 +1.8 1,401,999 + BBR_RS16285 0.89 +3.1 1,402,029 + +0.9 1,402,037 - +3.6 1,402,037 - +2.4 1,402,121 + +0.5 1,402,197 + -1.6 1,402,216 - -1.2 1,402,338 + -1.7 1,402,338 + +1.4 1,402,338 - +3.6 1,402,436 + BBR_RS16290 0.19 -2.0 1,402,444 - BBR_RS16290 0.20 -2.1 1,402,476 - BBR_RS16290 0.23 +1.2 1,402,477 - BBR_RS16290 0.24 -1.7 1,402,511 - BBR_RS16290 0.28 +0.1 1,402,535 + BBR_RS16290 0.30 -3.2 1,402,543 - BBR_RS16290 0.31 +0.2 1,402,543 - BBR_RS16290 0.31 -1.3 1,402,550 + BBR_RS16290 0.32 -1.3 1,402,555 + BBR_RS16290 0.33 +0.9 1,402,558 - BBR_RS16290 0.33 -2.9 1,402,649 - BBR_RS16290 0.44 -1.9 1,402,649 - BBR_RS16290 0.44 +4.7 1,402,649 - BBR_RS16290 0.44 -1.4 1,402,649 - BBR_RS16290 0.44 -2.1 1,402,649 - BBR_RS16290 0.44 +2.6 1,402,690 + BBR_RS16290 0.49 -1.0 1,402,690 + BBR_RS16290 0.49 -1.7 1,402,690 + BBR_RS16290 0.49 +1.7 1,402,690 + BBR_RS16290 0.49 +2.7 1,402,698 - BBR_RS16290 0.50 +2.0 1,402,711 + BBR_RS16290 0.51 -1.7 1,402,711 + BBR_RS16290 0.51 -2.1 1,402,711 + BBR_RS16290 0.51 +0.2 1,402,719 - BBR_RS16290 0.52 -1.8 1,402,719 - BBR_RS16290 0.52 -1.4 1,402,728 + BBR_RS16290 0.53 +0.2 1,402,728 + BBR_RS16290 0.53 -1.2 1,402,728 + BBR_RS16290 0.53 +0.3 1,402,728 + BBR_RS16290 0.53 +0.4 1,402,736 - BBR_RS16290 0.54 +2.3 1,402,785 + BBR_RS16290 0.60 +1.6 1,402,791 + BBR_RS16290 0.61 -2.2 1,402,817 - BBR_RS16290 0.64 -1.4 1,402,853 - BBR_RS16290 0.68 -1.5 1,402,853 - BBR_RS16290 0.68 +1.2 1,402,900 - BBR_RS16290 0.74 +1.4 1,402,921 + BBR_RS16290 0.76 -1.6 1,402,929 - BBR_RS16290 0.77 -2.3 1,402,990 - BBR_RS16290 0.84 -1.0 1,403,019 + BBR_RS16290 0.88 -0.6 1,403,032 - BBR_RS16290 0.89 +1.7
Or see this region's nucleotide sequence