Experiment: CDM1 without Phenylalanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15450 and BBR_RS15455 are separated by 3 nucleotides BBR_RS15455 and BBR_RS15460 are separated by 107 nucleotides BBR_RS15460 and BBR_RS15465 are separated by 367 nucleotides
BBR_RS15450: BBR_RS15450 - dihydroorotate dehydrogenase, at 1,237,604 to 1,238,575
_RS15450
BBR_RS15455: BBR_RS15455 - orotate phosphoribosyltransferase, at 1,238,579 to 1,239,274
_RS15455
BBR_RS15460: BBR_RS15460 - hypothetical protein, at 1,239,382 to 1,239,570
_RS15460
BBR_RS15465: BBR_RS15465 - DUF2974 domain-containing protein, at 1,239,938 to 1,241,059
_RS15465
Position (kb)
1239
1240 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1238.406 kb on + strand, within BBR_RS15450 at 1238.425 kb on + strand, within BBR_RS15450 at 1238.556 kb on + strand at 1238.652 kb on + strand, within BBR_RS15455 at 1238.866 kb on + strand, within BBR_RS15455 at 1238.866 kb on + strand, within BBR_RS15455 at 1238.941 kb on + strand, within BBR_RS15455 at 1239.030 kb on - strand, within BBR_RS15455 at 1239.384 kb on + strand at 1239.398 kb on + strand at 1239.398 kb on + strand at 1239.398 kb on + strand at 1239.404 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.485 kb on - strand, within BBR_RS15460 at 1239.560 kb on - strand at 1239.662 kb on - strand at 1239.677 kb on + strand at 1239.773 kb on - strand at 1239.846 kb on + strand at 1239.846 kb on + strand at 1239.846 kb on + strand at 1239.854 kb on - strand at 1239.929 kb on - strand at 1239.933 kb on + strand at 1239.991 kb on + strand at 1240.006 kb on + strand at 1240.104 kb on - strand, within BBR_RS15465 at 1240.113 kb on - strand, within BBR_RS15465 at 1240.160 kb on + strand, within BBR_RS15465 at 1240.284 kb on - strand, within BBR_RS15465 at 1240.315 kb on + strand, within BBR_RS15465 at 1240.323 kb on - strand, within BBR_RS15465 at 1240.446 kb on - strand, within BBR_RS15465
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Phenylalanine remove 1,238,406 + BBR_RS15450 0.83 +5.9 1,238,425 + BBR_RS15450 0.84 +6.3 1,238,556 + -1.2 1,238,652 + BBR_RS15455 0.10 +4.1 1,238,866 + BBR_RS15455 0.41 +5.7 1,238,866 + BBR_RS15455 0.41 +5.1 1,238,941 + BBR_RS15455 0.52 +2.4 1,239,030 - BBR_RS15455 0.65 +1.4 1,239,384 + +0.6 1,239,398 + -1.5 1,239,398 + +0.7 1,239,398 + +1.4 1,239,404 - BBR_RS15460 0.12 -1.2 1,239,406 - BBR_RS15460 0.13 -2.3 1,239,406 - BBR_RS15460 0.13 -2.3 1,239,406 - BBR_RS15460 0.13 +2.2 1,239,485 - BBR_RS15460 0.54 -2.6 1,239,560 - +0.3 1,239,662 - +1.9 1,239,677 + +1.7 1,239,773 - -1.9 1,239,846 + +0.3 1,239,846 + +0.1 1,239,846 + -0.5 1,239,854 - +5.1 1,239,929 - -1.3 1,239,933 + +2.8 1,239,991 + -3.1 1,240,006 + -1.3 1,240,104 - BBR_RS15465 0.15 -2.0 1,240,113 - BBR_RS15465 0.16 +0.6 1,240,160 + BBR_RS15465 0.20 -0.3 1,240,284 - BBR_RS15465 0.31 +1.5 1,240,315 + BBR_RS15465 0.34 -1.7 1,240,323 - BBR_RS15465 0.34 -0.1 1,240,446 - BBR_RS15465 0.45 +1.6
Or see this region's nucleotide sequence