Experiment: CDM1 without Phenylalanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20840 and BBR_RS14685 are separated by 280 nucleotides BBR_RS14685 and BBR_RS14690 are separated by 237 nucleotides BBR_RS14690 and BBR_RS20845 are separated by 93 nucleotides BBR_RS20845 and BBR_RS14700 are separated by 216 nucleotides
BBR_RS20840: BBR_RS20840 - hypothetical protein, at 1,070,575 to 1,070,760
_RS20840
BBR_RS14685: BBR_RS14685 - YccF domain-containing protein, at 1,071,041 to 1,071,406
_RS14685
BBR_RS14690: BBR_RS14690 - class I SAM-dependent methyltransferase, at 1,071,644 to 1,072,513
_RS14690
BBR_RS20845: BBR_RS20845 - cupin, at 1,072,607 to 1,073,221
_RS20845
BBR_RS14700: BBR_RS14700 - type II toxin-antitoxin system PemK/MazF family toxin, at 1,073,438 to 1,073,761
_RS14700
Position (kb)
1071
1072
1073 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1070.673 kb on - strand, within BBR_RS20840 at 1070.715 kb on + strand, within BBR_RS20840 at 1070.843 kb on + strand at 1070.848 kb on + strand at 1070.848 kb on + strand at 1070.851 kb on - strand at 1070.853 kb on + strand at 1070.918 kb on + strand at 1070.918 kb on + strand at 1070.926 kb on - strand at 1071.014 kb on + strand at 1071.022 kb on - strand at 1071.048 kb on + strand at 1071.048 kb on + strand at 1071.048 kb on + strand at 1071.056 kb on - strand at 1071.056 kb on - strand at 1071.056 kb on - strand at 1071.100 kb on + strand, within BBR_RS14685 at 1071.125 kb on - strand, within BBR_RS14685 at 1071.129 kb on + strand, within BBR_RS14685 at 1071.161 kb on + strand, within BBR_RS14685 at 1071.161 kb on + strand, within BBR_RS14685 at 1071.190 kb on - strand, within BBR_RS14685 at 1071.209 kb on - strand, within BBR_RS14685 at 1071.209 kb on - strand, within BBR_RS14685 at 1071.262 kb on + strand, within BBR_RS14685 at 1071.340 kb on + strand, within BBR_RS14685 at 1071.340 kb on + strand, within BBR_RS14685 at 1071.348 kb on - strand, within BBR_RS14685 at 1071.400 kb on - strand at 1071.520 kb on - strand at 1071.648 kb on + strand at 1071.655 kb on + strand at 1071.656 kb on - strand at 1071.656 kb on - strand at 1071.656 kb on - strand at 1071.682 kb on - strand at 1071.682 kb on - strand at 1071.733 kb on + strand, within BBR_RS14690 at 1071.741 kb on + strand, within BBR_RS14690 at 1071.748 kb on + strand, within BBR_RS14690 at 1071.748 kb on + strand, within BBR_RS14690 at 1071.756 kb on - strand, within BBR_RS14690 at 1071.756 kb on - strand, within BBR_RS14690 at 1071.881 kb on + strand, within BBR_RS14690 at 1071.881 kb on + strand, within BBR_RS14690 at 1071.919 kb on + strand, within BBR_RS14690 at 1071.972 kb on - strand, within BBR_RS14690 at 1071.976 kb on - strand, within BBR_RS14690 at 1071.993 kb on - strand, within BBR_RS14690 at 1072.010 kb on - strand, within BBR_RS14690 at 1072.030 kb on - strand, within BBR_RS14690 at 1072.070 kb on + strand, within BBR_RS14690 at 1072.131 kb on - strand, within BBR_RS14690 at 1072.131 kb on - strand, within BBR_RS14690 at 1072.164 kb on + strand, within BBR_RS14690 at 1072.174 kb on + strand, within BBR_RS14690 at 1072.185 kb on + strand, within BBR_RS14690 at 1072.211 kb on - strand, within BBR_RS14690 at 1072.213 kb on + strand, within BBR_RS14690 at 1072.221 kb on - strand, within BBR_RS14690 at 1072.290 kb on - strand, within BBR_RS14690 at 1072.304 kb on - strand, within BBR_RS14690 at 1072.306 kb on + strand, within BBR_RS14690 at 1072.367 kb on + strand, within BBR_RS14690 at 1072.367 kb on + strand, within BBR_RS14690 at 1072.374 kb on + strand, within BBR_RS14690 at 1072.400 kb on + strand, within BBR_RS14690 at 1072.417 kb on + strand, within BBR_RS14690 at 1072.442 kb on + strand at 1072.445 kb on - strand at 1072.445 kb on - strand at 1072.445 kb on - strand at 1072.445 kb on - strand at 1072.449 kb on + strand at 1072.452 kb on - strand at 1072.485 kb on + strand at 1072.611 kb on - strand at 1072.611 kb on - strand at 1072.632 kb on - strand at 1072.686 kb on - strand, within BBR_RS20845 at 1072.730 kb on - strand, within BBR_RS20845 at 1072.810 kb on - strand, within BBR_RS20845 at 1072.846 kb on - strand, within BBR_RS20845 at 1072.874 kb on + strand, within BBR_RS20845 at 1072.874 kb on + strand, within BBR_RS20845 at 1072.882 kb on - strand, within BBR_RS20845 at 1072.921 kb on + strand, within BBR_RS20845 at 1072.966 kb on - strand, within BBR_RS20845 at 1072.968 kb on + strand, within BBR_RS20845 at 1072.969 kb on - strand, within BBR_RS20845 at 1072.971 kb on - strand, within BBR_RS20845 at 1072.981 kb on - strand, within BBR_RS20845 at 1072.998 kb on - strand, within BBR_RS20845 at 1073.000 kb on + strand, within BBR_RS20845 at 1073.003 kb on - strand, within BBR_RS20845 at 1073.020 kb on + strand, within BBR_RS20845 at 1073.035 kb on - strand, within BBR_RS20845 at 1073.079 kb on - strand, within BBR_RS20845 at 1073.135 kb on + strand, within BBR_RS20845 at 1073.135 kb on + strand, within BBR_RS20845 at 1073.141 kb on - strand, within BBR_RS20845 at 1073.172 kb on - strand at 1073.172 kb on - strand at 1073.182 kb on + strand at 1073.243 kb on + strand at 1073.252 kb on - strand at 1073.270 kb on - strand at 1073.280 kb on - strand at 1073.306 kb on - strand at 1073.347 kb on - strand at 1073.363 kb on - strand at 1073.379 kb on + strand at 1073.379 kb on + strand at 1073.386 kb on - strand at 1073.389 kb on + strand at 1073.408 kb on - strand at 1073.436 kb on + strand at 1073.441 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Phenylalanine remove 1,070,673 - BBR_RS20840 0.53 +0.4 1,070,715 + BBR_RS20840 0.75 +1.9 1,070,843 + +0.9 1,070,848 + +2.6 1,070,848 + -0.8 1,070,851 - +0.3 1,070,853 + +0.5 1,070,918 + -0.1 1,070,918 + +0.0 1,070,926 - -2.1 1,071,014 + +3.9 1,071,022 - +0.3 1,071,048 + -1.5 1,071,048 + -0.5 1,071,048 + +1.3 1,071,056 - +0.4 1,071,056 - +1.7 1,071,056 - +1.5 1,071,100 + BBR_RS14685 0.16 +1.9 1,071,125 - BBR_RS14685 0.23 +1.9 1,071,129 + BBR_RS14685 0.24 -0.0 1,071,161 + BBR_RS14685 0.33 +1.1 1,071,161 + BBR_RS14685 0.33 +0.1 1,071,190 - BBR_RS14685 0.41 -0.4 1,071,209 - BBR_RS14685 0.46 -0.6 1,071,209 - BBR_RS14685 0.46 -0.9 1,071,262 + BBR_RS14685 0.60 +2.5 1,071,340 + BBR_RS14685 0.82 -1.6 1,071,340 + BBR_RS14685 0.82 +2.3 1,071,348 - BBR_RS14685 0.84 -1.1 1,071,400 - +3.8 1,071,520 - -2.5 1,071,648 + -1.0 1,071,655 + -1.7 1,071,656 - -0.4 1,071,656 - +1.2 1,071,656 - +1.6 1,071,682 - +1.9 1,071,682 - -1.1 1,071,733 + BBR_RS14690 0.10 -1.4 1,071,741 + BBR_RS14690 0.11 +1.0 1,071,748 + BBR_RS14690 0.12 -2.2 1,071,748 + BBR_RS14690 0.12 -1.2 1,071,756 - BBR_RS14690 0.13 +0.9 1,071,756 - BBR_RS14690 0.13 -1.9 1,071,881 + BBR_RS14690 0.27 -1.0 1,071,881 + BBR_RS14690 0.27 +2.1 1,071,919 + BBR_RS14690 0.32 -1.1 1,071,972 - BBR_RS14690 0.38 -1.9 1,071,976 - BBR_RS14690 0.38 -1.2 1,071,993 - BBR_RS14690 0.40 -2.3 1,072,010 - BBR_RS14690 0.42 -1.6 1,072,030 - BBR_RS14690 0.44 -2.7 1,072,070 + BBR_RS14690 0.49 -0.4 1,072,131 - BBR_RS14690 0.56 -0.6 1,072,131 - BBR_RS14690 0.56 -0.1 1,072,164 + BBR_RS14690 0.60 +2.2 1,072,174 + BBR_RS14690 0.61 +2.2 1,072,185 + BBR_RS14690 0.62 +1.2 1,072,211 - BBR_RS14690 0.65 +0.8 1,072,213 + BBR_RS14690 0.65 +0.6 1,072,221 - BBR_RS14690 0.66 -0.1 1,072,290 - BBR_RS14690 0.74 +0.3 1,072,304 - BBR_RS14690 0.76 -0.2 1,072,306 + BBR_RS14690 0.76 +1.4 1,072,367 + BBR_RS14690 0.83 -1.6 1,072,367 + BBR_RS14690 0.83 -1.1 1,072,374 + BBR_RS14690 0.84 -2.0 1,072,400 + BBR_RS14690 0.87 +2.5 1,072,417 + BBR_RS14690 0.89 -0.9 1,072,442 + -2.7 1,072,445 - -1.3 1,072,445 - -1.4 1,072,445 - +0.1 1,072,445 - -0.6 1,072,449 + +2.1 1,072,452 - -2.8 1,072,485 + -1.5 1,072,611 - +2.4 1,072,611 - +0.9 1,072,632 - -0.7 1,072,686 - BBR_RS20845 0.13 -2.2 1,072,730 - BBR_RS20845 0.20 +2.4 1,072,810 - BBR_RS20845 0.33 +4.1 1,072,846 - BBR_RS20845 0.39 -2.3 1,072,874 + BBR_RS20845 0.43 +0.7 1,072,874 + BBR_RS20845 0.43 -1.7 1,072,882 - BBR_RS20845 0.45 -0.5 1,072,921 + BBR_RS20845 0.51 +0.9 1,072,966 - BBR_RS20845 0.58 -1.8 1,072,968 + BBR_RS20845 0.59 +1.8 1,072,969 - BBR_RS20845 0.59 +1.9 1,072,971 - BBR_RS20845 0.59 -0.1 1,072,981 - BBR_RS20845 0.61 +2.2 1,072,998 - BBR_RS20845 0.64 -0.4 1,073,000 + BBR_RS20845 0.64 -0.4 1,073,003 - BBR_RS20845 0.64 -0.6 1,073,020 + BBR_RS20845 0.67 -2.0 1,073,035 - BBR_RS20845 0.70 +2.6 1,073,079 - BBR_RS20845 0.77 +3.2 1,073,135 + BBR_RS20845 0.86 +0.4 1,073,135 + BBR_RS20845 0.86 +0.6 1,073,141 - BBR_RS20845 0.87 +2.2 1,073,172 - -1.0 1,073,172 - +3.2 1,073,182 + -1.0 1,073,243 + +0.0 1,073,252 - -0.9 1,073,270 - -2.0 1,073,280 - +3.0 1,073,306 - +3.3 1,073,347 - +2.5 1,073,363 - -1.4 1,073,379 + -1.9 1,073,379 + -2.4 1,073,386 - +1.6 1,073,389 + +2.2 1,073,408 - +1.4 1,073,436 + +1.0 1,073,441 + -0.2
Or see this region's nucleotide sequence