Experiment: CDM1 without Phenylalanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11290 and BBR_RS11295 are separated by 333 nucleotides BBR_RS11295 and BBR_RS11300 are separated by 197 nucleotides BBR_RS11300 and BBR_RS11305 are separated by 97 nucleotides
BBR_RS11290: BBR_RS11290 - DUF1275 domain-containing protein, at 278,411 to 279,259
_RS11290
BBR_RS11295: BBR_RS11295 - transcriptional regulator, at 279,593 to 279,922
_RS11295
BBR_RS11300: BBR_RS11300 - hypothetical protein, at 280,120 to 280,581
_RS11300
BBR_RS11305: BBR_RS11305 - divergent AAA domain protein, at 280,679 to 282,331
_RS11305
Position (kb) 279
280 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 278.602 kb on + strand, within BBR_RS11290 at 278.602 kb on + strand, within BBR_RS11290 at 278.602 kb on + strand, within BBR_RS11290 at 278.610 kb on - strand, within BBR_RS11290 at 278.616 kb on + strand, within BBR_RS11290 at 278.616 kb on + strand, within BBR_RS11290 at 278.622 kb on - strand, within BBR_RS11290 at 278.634 kb on + strand, within BBR_RS11290 at 278.644 kb on + strand, within BBR_RS11290 at 278.644 kb on + strand, within BBR_RS11290 at 278.656 kb on + strand, within BBR_RS11290 at 278.661 kb on + strand, within BBR_RS11290 at 278.661 kb on + strand, within BBR_RS11290 at 278.661 kb on + strand, within BBR_RS11290 at 278.661 kb on + strand, within BBR_RS11290 at 278.664 kb on - strand, within BBR_RS11290 at 278.664 kb on - strand, within BBR_RS11290 at 278.669 kb on - strand, within BBR_RS11290 at 278.669 kb on - strand, within BBR_RS11290 at 278.669 kb on - strand, within BBR_RS11290 at 278.669 kb on - strand, within BBR_RS11290 at 278.669 kb on - strand, within BBR_RS11290 at 278.669 kb on - strand, within BBR_RS11290 at 278.672 kb on + strand, within BBR_RS11290 at 278.672 kb on + strand, within BBR_RS11290 at 278.680 kb on - strand, within BBR_RS11290 at 278.697 kb on + strand, within BBR_RS11290 at 278.716 kb on + strand, within BBR_RS11290 at 278.733 kb on + strand, within BBR_RS11290 at 278.733 kb on + strand, within BBR_RS11290 at 278.734 kb on - strand, within BBR_RS11290 at 278.745 kb on + strand, within BBR_RS11290 at 278.753 kb on - strand, within BBR_RS11290 at 278.755 kb on + strand, within BBR_RS11290 at 278.755 kb on - strand, within BBR_RS11290 at 278.770 kb on + strand, within BBR_RS11290 at 278.772 kb on - strand, within BBR_RS11290 at 278.780 kb on + strand, within BBR_RS11290 at 278.780 kb on + strand, within BBR_RS11290 at 278.780 kb on + strand, within BBR_RS11290 at 278.780 kb on + strand, within BBR_RS11290 at 278.780 kb on + strand, within BBR_RS11290 at 278.791 kb on + strand, within BBR_RS11290 at 278.791 kb on + strand, within BBR_RS11290 at 278.791 kb on + strand, within BBR_RS11290 at 278.791 kb on + strand, within BBR_RS11290 at 278.791 kb on + strand, within BBR_RS11290 at 278.799 kb on - strand, within BBR_RS11290 at 278.807 kb on + strand, within BBR_RS11290 at 278.807 kb on + strand, within BBR_RS11290 at 278.809 kb on + strand, within BBR_RS11290 at 278.815 kb on - strand, within BBR_RS11290 at 278.815 kb on - strand, within BBR_RS11290 at 278.821 kb on + strand, within BBR_RS11290 at 278.821 kb on + strand, within BBR_RS11290 at 278.824 kb on - strand, within BBR_RS11290 at 278.828 kb on - strand, within BBR_RS11290 at 278.829 kb on - strand, within BBR_RS11290 at 278.829 kb on - strand, within BBR_RS11290 at 278.835 kb on + strand, within BBR_RS11290 at 278.835 kb on + strand, within BBR_RS11290 at 278.838 kb on - strand, within BBR_RS11290 at 278.871 kb on + strand, within BBR_RS11290 at 278.874 kb on - strand, within BBR_RS11290 at 278.898 kb on - strand, within BBR_RS11290 at 278.908 kb on - strand, within BBR_RS11290 at 278.936 kb on + strand, within BBR_RS11290 at 278.936 kb on + strand, within BBR_RS11290 at 278.953 kb on - strand, within BBR_RS11290 at 279.010 kb on + strand, within BBR_RS11290 at 279.010 kb on + strand, within BBR_RS11290 at 279.053 kb on + strand, within BBR_RS11290 at 279.058 kb on + strand, within BBR_RS11290 at 279.058 kb on + strand, within BBR_RS11290 at 279.058 kb on + strand, within BBR_RS11290 at 279.066 kb on - strand, within BBR_RS11290 at 279.109 kb on - strand, within BBR_RS11290 at 279.122 kb on + strand, within BBR_RS11290 at 279.124 kb on - strand, within BBR_RS11290 at 279.127 kb on + strand, within BBR_RS11290 at 279.140 kb on - strand, within BBR_RS11290 at 279.175 kb on + strand at 279.175 kb on + strand at 279.175 kb on + strand at 279.176 kb on + strand at 279.191 kb on + strand at 279.199 kb on - strand at 279.201 kb on + strand at 279.204 kb on - strand at 279.206 kb on - strand at 279.247 kb on + strand at 279.255 kb on - strand at 279.255 kb on - strand at 279.320 kb on + strand at 279.325 kb on + strand at 279.330 kb on + strand at 279.333 kb on + strand at 279.333 kb on + strand at 279.333 kb on + strand at 279.333 kb on + strand at 279.338 kb on + strand at 279.341 kb on - strand at 279.341 kb on - strand at 279.361 kb on + strand at 279.369 kb on - strand at 279.398 kb on + strand at 279.398 kb on - strand at 279.401 kb on + strand at 279.401 kb on + strand at 279.401 kb on - strand at 279.403 kb on - strand at 279.406 kb on - strand at 279.406 kb on - strand at 279.406 kb on - strand at 279.428 kb on - strand at 279.457 kb on + strand at 279.464 kb on + strand at 279.480 kb on + strand at 279.488 kb on - strand at 279.493 kb on - strand at 279.495 kb on - strand at 279.498 kb on + strand at 279.524 kb on - strand at 279.531 kb on + strand at 279.546 kb on + strand at 279.546 kb on + strand at 279.546 kb on + strand at 279.554 kb on - strand at 279.559 kb on - strand at 279.574 kb on - strand at 279.585 kb on - strand at 279.590 kb on + strand at 279.603 kb on + strand at 279.603 kb on + strand at 279.609 kb on + strand at 279.609 kb on + strand at 279.609 kb on + strand at 279.610 kb on - strand at 279.611 kb on - strand at 279.611 kb on - strand at 279.611 kb on - strand at 279.624 kb on + strand at 279.632 kb on - strand, within BBR_RS11295 at 279.632 kb on - strand, within BBR_RS11295 at 279.635 kb on - strand, within BBR_RS11295 at 279.651 kb on + strand, within BBR_RS11295 at 279.659 kb on + strand, within BBR_RS11295 at 279.659 kb on - strand, within BBR_RS11295 at 279.667 kb on - strand, within BBR_RS11295 at 279.672 kb on - strand, within BBR_RS11295 at 279.710 kb on - strand, within BBR_RS11295 at 279.716 kb on - strand, within BBR_RS11295 at 279.722 kb on + strand, within BBR_RS11295 at 279.730 kb on - strand, within BBR_RS11295 at 279.732 kb on - strand, within BBR_RS11295 at 279.754 kb on + strand, within BBR_RS11295 at 279.754 kb on + strand, within BBR_RS11295 at 279.754 kb on + strand, within BBR_RS11295 at 279.754 kb on + strand, within BBR_RS11295 at 279.754 kb on + strand, within BBR_RS11295 at 279.754 kb on + strand, within BBR_RS11295 at 279.762 kb on - strand, within BBR_RS11295 at 279.790 kb on - strand, within BBR_RS11295 at 279.792 kb on - strand, within BBR_RS11295 at 279.810 kb on - strand, within BBR_RS11295 at 279.819 kb on + strand, within BBR_RS11295 at 279.922 kb on + strand at 279.924 kb on + strand at 279.981 kb on + strand at 279.982 kb on - strand at 280.004 kb on + strand at 280.053 kb on - strand at 280.103 kb on + strand at 280.106 kb on + strand at 280.106 kb on + strand at 280.106 kb on - strand at 280.111 kb on - strand at 280.117 kb on + strand at 280.124 kb on - strand at 280.128 kb on + strand at 280.131 kb on + strand at 280.180 kb on - strand, within BBR_RS11300 at 280.182 kb on - strand, within BBR_RS11300 at 280.182 kb on - strand, within BBR_RS11300 at 280.196 kb on + strand, within BBR_RS11300 at 280.196 kb on + strand, within BBR_RS11300 at 280.206 kb on - strand, within BBR_RS11300 at 280.208 kb on + strand, within BBR_RS11300 at 280.216 kb on - strand, within BBR_RS11300 at 280.216 kb on - strand, within BBR_RS11300 at 280.221 kb on - strand, within BBR_RS11300 at 280.235 kb on - strand, within BBR_RS11300 at 280.243 kb on - strand, within BBR_RS11300 at 280.254 kb on + strand, within BBR_RS11300 at 280.283 kb on - strand, within BBR_RS11300 at 280.285 kb on + strand, within BBR_RS11300 at 280.303 kb on - strand, within BBR_RS11300 at 280.345 kb on + strand, within BBR_RS11300 at 280.345 kb on + strand, within BBR_RS11300 at 280.361 kb on - strand, within BBR_RS11300 at 280.361 kb on - strand, within BBR_RS11300 at 280.383 kb on + strand, within BBR_RS11300 at 280.432 kb on - strand, within BBR_RS11300 at 280.432 kb on - strand, within BBR_RS11300 at 280.432 kb on - strand, within BBR_RS11300 at 280.439 kb on - strand, within BBR_RS11300 at 280.454 kb on + strand, within BBR_RS11300 at 280.456 kb on + strand, within BBR_RS11300 at 280.456 kb on + strand, within BBR_RS11300 at 280.459 kb on + strand, within BBR_RS11300 at 280.462 kb on - strand, within BBR_RS11300 at 280.464 kb on - strand, within BBR_RS11300 at 280.471 kb on + strand, within BBR_RS11300 at 280.498 kb on - strand, within BBR_RS11300 at 280.498 kb on - strand, within BBR_RS11300 at 280.508 kb on - strand, within BBR_RS11300 at 280.519 kb on - strand, within BBR_RS11300 at 280.536 kb on + strand at 280.536 kb on + strand at 280.539 kb on - strand at 280.541 kb on + strand at 280.546 kb on + strand at 280.592 kb on + strand at 280.602 kb on + strand at 280.641 kb on - strand at 280.645 kb on + strand at 280.645 kb on + strand at 280.647 kb on + strand at 280.647 kb on + strand at 280.647 kb on + strand at 280.655 kb on - strand at 280.669 kb on - strand at 280.669 kb on - strand at 280.674 kb on + strand at 280.682 kb on - strand at 280.688 kb on + strand at 280.756 kb on + strand at 280.764 kb on - strand at 280.764 kb on - strand at 280.764 kb on - strand at 280.774 kb on - strand at 280.777 kb on + strand at 280.791 kb on + strand at 280.791 kb on - strand at 280.799 kb on + strand at 280.801 kb on + strand at 280.807 kb on - strand at 280.807 kb on - strand at 280.839 kb on - strand at 280.841 kb on + strand at 280.847 kb on + strand, within BBR_RS11305 at 280.866 kb on + strand, within BBR_RS11305 at 280.887 kb on + strand, within BBR_RS11305 at 280.888 kb on + strand, within BBR_RS11305 at 280.890 kb on + strand, within BBR_RS11305 at 280.890 kb on + strand, within BBR_RS11305 at 280.896 kb on - strand, within BBR_RS11305 at 280.896 kb on - strand, within BBR_RS11305 at 280.904 kb on + strand, within BBR_RS11305
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Phenylalanine remove 278,602 + BBR_RS11290 0.22 +0.7 278,602 + BBR_RS11290 0.22 -2.2 278,602 + BBR_RS11290 0.22 -0.6 278,610 - BBR_RS11290 0.23 +1.3 278,616 + BBR_RS11290 0.24 +3.4 278,616 + BBR_RS11290 0.24 +1.4 278,622 - BBR_RS11290 0.25 -2.0 278,634 + BBR_RS11290 0.26 +2.4 278,644 + BBR_RS11290 0.27 -1.7 278,644 + BBR_RS11290 0.27 -2.9 278,656 + BBR_RS11290 0.29 -0.2 278,661 + BBR_RS11290 0.29 -1.4 278,661 + BBR_RS11290 0.29 -1.4 278,661 + BBR_RS11290 0.29 +1.6 278,661 + BBR_RS11290 0.29 -2.2 278,664 - BBR_RS11290 0.30 +1.2 278,664 - BBR_RS11290 0.30 +3.0 278,669 - BBR_RS11290 0.30 +3.6 278,669 - BBR_RS11290 0.30 +3.5 278,669 - BBR_RS11290 0.30 +1.9 278,669 - BBR_RS11290 0.30 -2.6 278,669 - BBR_RS11290 0.30 -1.7 278,669 - BBR_RS11290 0.30 +0.0 278,672 + BBR_RS11290 0.31 -2.8 278,672 + BBR_RS11290 0.31 +1.8 278,680 - BBR_RS11290 0.32 -2.2 278,697 + BBR_RS11290 0.34 +0.3 278,716 + BBR_RS11290 0.36 -1.9 278,733 + BBR_RS11290 0.38 +2.6 278,733 + BBR_RS11290 0.38 +1.4 278,734 - BBR_RS11290 0.38 -0.3 278,745 + BBR_RS11290 0.39 -2.1 278,753 - BBR_RS11290 0.40 -2.8 278,755 + BBR_RS11290 0.41 -0.1 278,755 - BBR_RS11290 0.41 -1.4 278,770 + BBR_RS11290 0.42 -1.2 278,772 - BBR_RS11290 0.43 -3.2 278,780 + BBR_RS11290 0.43 +0.4 278,780 + BBR_RS11290 0.43 +1.1 278,780 + BBR_RS11290 0.43 +2.6 278,780 + BBR_RS11290 0.43 +0.6 278,780 + BBR_RS11290 0.43 +2.4 278,791 + BBR_RS11290 0.45 -1.2 278,791 + BBR_RS11290 0.45 -2.9 278,791 + BBR_RS11290 0.45 -2.0 278,791 + BBR_RS11290 0.45 -0.9 278,791 + BBR_RS11290 0.45 +1.7 278,799 - BBR_RS11290 0.46 +2.6 278,807 + BBR_RS11290 0.47 +1.9 278,807 + BBR_RS11290 0.47 -1.1 278,809 + BBR_RS11290 0.47 +0.2 278,815 - BBR_RS11290 0.48 -0.7 278,815 - BBR_RS11290 0.48 -1.9 278,821 + BBR_RS11290 0.48 +1.9 278,821 + BBR_RS11290 0.48 -3.9 278,824 - BBR_RS11290 0.49 -1.3 278,828 - BBR_RS11290 0.49 +0.4 278,829 - BBR_RS11290 0.49 -0.7 278,829 - BBR_RS11290 0.49 +0.8 278,835 + BBR_RS11290 0.50 -2.1 278,835 + BBR_RS11290 0.50 -1.4 278,838 - BBR_RS11290 0.50 +0.2 278,871 + BBR_RS11290 0.54 -0.7 278,874 - BBR_RS11290 0.55 -0.6 278,898 - BBR_RS11290 0.57 +2.3 278,908 - BBR_RS11290 0.59 -2.3 278,936 + BBR_RS11290 0.62 -0.2 278,936 + BBR_RS11290 0.62 -0.5 278,953 - BBR_RS11290 0.64 +2.0 279,010 + BBR_RS11290 0.71 -1.9 279,010 + BBR_RS11290 0.71 +1.6 279,053 + BBR_RS11290 0.76 -1.6 279,058 + BBR_RS11290 0.76 -1.8 279,058 + BBR_RS11290 0.76 -1.3 279,058 + BBR_RS11290 0.76 +1.0 279,066 - BBR_RS11290 0.77 -0.2 279,109 - BBR_RS11290 0.82 +0.1 279,122 + BBR_RS11290 0.84 -1.4 279,124 - BBR_RS11290 0.84 +0.0 279,127 + BBR_RS11290 0.84 +2.8 279,140 - BBR_RS11290 0.86 +2.8 279,175 + +2.2 279,175 + -1.9 279,175 + +3.0 279,176 + -1.3 279,191 + -2.1 279,199 - -2.2 279,201 + -1.8 279,204 - -3.3 279,206 - +1.5 279,247 + -1.1 279,255 - +1.3 279,255 - -2.0 279,320 + -1.7 279,325 + +0.4 279,330 + -1.7 279,333 + -3.1 279,333 + -0.0 279,333 + -2.7 279,333 + +0.1 279,338 + +0.1 279,341 - -2.0 279,341 - +1.6 279,361 + +0.2 279,369 - -1.2 279,398 + +0.5 279,398 - +1.0 279,401 + +1.8 279,401 + -1.1 279,401 - -0.6 279,403 - +0.9 279,406 - -2.0 279,406 - -1.3 279,406 - +1.8 279,428 - -1.8 279,457 + +0.1 279,464 + +0.3 279,480 + +1.5 279,488 - -2.6 279,493 - +1.2 279,495 - +2.1 279,498 + +0.3 279,524 - +1.2 279,531 + -2.5 279,546 + -0.7 279,546 + -0.3 279,546 + -2.0 279,554 - +2.2 279,559 - +2.2 279,574 - +2.2 279,585 - -0.0 279,590 + -2.0 279,603 + +1.1 279,603 + -2.2 279,609 + -0.6 279,609 + +2.2 279,609 + -1.2 279,610 - +2.5 279,611 - -2.4 279,611 - -2.8 279,611 - +1.6 279,624 + -1.2 279,632 - BBR_RS11295 0.12 -1.3 279,632 - BBR_RS11295 0.12 -1.7 279,635 - BBR_RS11295 0.13 +2.1 279,651 + BBR_RS11295 0.18 +1.0 279,659 + BBR_RS11295 0.20 -0.1 279,659 - BBR_RS11295 0.20 +0.1 279,667 - BBR_RS11295 0.22 +0.3 279,672 - BBR_RS11295 0.24 -1.4 279,710 - BBR_RS11295 0.35 -1.6 279,716 - BBR_RS11295 0.37 +2.8 279,722 + BBR_RS11295 0.39 -1.2 279,730 - BBR_RS11295 0.42 -0.3 279,732 - BBR_RS11295 0.42 -0.9 279,754 + BBR_RS11295 0.49 +2.0 279,754 + BBR_RS11295 0.49 +0.6 279,754 + BBR_RS11295 0.49 -1.6 279,754 + BBR_RS11295 0.49 -0.1 279,754 + BBR_RS11295 0.49 -1.3 279,754 + BBR_RS11295 0.49 -1.3 279,762 - BBR_RS11295 0.51 +0.2 279,790 - BBR_RS11295 0.60 -0.8 279,792 - BBR_RS11295 0.60 +1.1 279,810 - BBR_RS11295 0.66 +0.7 279,819 + BBR_RS11295 0.68 -1.2 279,922 + +0.2 279,924 + +0.3 279,981 + +0.9 279,982 - +2.2 280,004 + +0.1 280,053 - -0.9 280,103 + -0.4 280,106 + -2.2 280,106 + +3.9 280,106 - -1.4 280,111 - +1.1 280,117 + -2.2 280,124 - +2.5 280,128 + +0.4 280,131 + -1.6 280,180 - BBR_RS11300 0.13 +1.5 280,182 - BBR_RS11300 0.13 -2.0 280,182 - BBR_RS11300 0.13 -1.5 280,196 + BBR_RS11300 0.16 -1.2 280,196 + BBR_RS11300 0.16 +2.2 280,206 - BBR_RS11300 0.19 +1.8 280,208 + BBR_RS11300 0.19 +1.0 280,216 - BBR_RS11300 0.21 +0.4 280,216 - BBR_RS11300 0.21 +1.1 280,221 - BBR_RS11300 0.22 -2.1 280,235 - BBR_RS11300 0.25 +3.2 280,243 - BBR_RS11300 0.27 -1.1 280,254 + BBR_RS11300 0.29 +2.4 280,283 - BBR_RS11300 0.35 -0.3 280,285 + BBR_RS11300 0.36 +1.2 280,303 - BBR_RS11300 0.40 +4.0 280,345 + BBR_RS11300 0.49 -2.4 280,345 + BBR_RS11300 0.49 -3.0 280,361 - BBR_RS11300 0.52 -1.6 280,361 - BBR_RS11300 0.52 +2.9 280,383 + BBR_RS11300 0.57 +2.2 280,432 - BBR_RS11300 0.68 -1.3 280,432 - BBR_RS11300 0.68 +1.5 280,432 - BBR_RS11300 0.68 -1.0 280,439 - BBR_RS11300 0.69 -0.3 280,454 + BBR_RS11300 0.72 -1.0 280,456 + BBR_RS11300 0.73 -0.0 280,456 + BBR_RS11300 0.73 -2.3 280,459 + BBR_RS11300 0.73 +1.2 280,462 - BBR_RS11300 0.74 +2.6 280,464 - BBR_RS11300 0.74 -1.8 280,471 + BBR_RS11300 0.76 -1.3 280,498 - BBR_RS11300 0.82 -1.4 280,498 - BBR_RS11300 0.82 -0.4 280,508 - BBR_RS11300 0.84 +1.7 280,519 - BBR_RS11300 0.86 +2.5 280,536 + -2.3 280,536 + +1.7 280,539 - +1.4 280,541 + +0.3 280,546 + +1.5 280,592 + +1.1 280,602 + +1.7 280,641 - -1.1 280,645 + +0.6 280,645 + +0.6 280,647 + +0.3 280,647 + -2.8 280,647 + -0.7 280,655 - +0.6 280,669 - +0.6 280,669 - -1.4 280,674 + +2.0 280,682 - +0.9 280,688 + +2.5 280,756 + -1.9 280,764 - -0.1 280,764 - -1.6 280,764 - -1.9 280,774 - +1.8 280,777 + -2.9 280,791 + -0.2 280,791 - -0.2 280,799 + -2.4 280,801 + -3.4 280,807 - +1.7 280,807 - +1.3 280,839 - +0.7 280,841 + +1.2 280,847 + BBR_RS11305 0.10 -0.8 280,866 + BBR_RS11305 0.11 +1.4 280,887 + BBR_RS11305 0.13 -1.7 280,888 + BBR_RS11305 0.13 +3.5 280,890 + BBR_RS11305 0.13 -1.3 280,890 + BBR_RS11305 0.13 -1.4 280,896 - BBR_RS11305 0.13 -1.1 280,896 - BBR_RS11305 0.13 -2.7 280,904 + BBR_RS11305 0.14 -0.2
Or see this region's nucleotide sequence