Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22955 and HSERO_RS22960 are separated by 122 nucleotides HSERO_RS22960 and HSERO_RS22965 are separated by 125 nucleotides HSERO_RS22965 and HSERO_RS22970 are separated by 15 nucleotides
HSERO_RS22955: HSERO_RS22955 - D-amino acid dehydrogenase, at 5,267,393 to 5,268,679
_RS22955
HSERO_RS22960: HSERO_RS22960 - glyoxalase, at 5,268,802 to 5,269,188
_RS22960
HSERO_RS22965: HSERO_RS22965 - activator of HSP90 ATPase, at 5,269,314 to 5,269,769
_RS22965
HSERO_RS22970: HSERO_RS22970 - membrane protein, at 5,269,785 to 5,271,455
_RS22970
Position (kb) 5269
5270 Strain fitness (log2 ratio) -2
-1
0
1 at 5268.632 kb on + strand at 5268.759 kb on - strand at 5268.791 kb on - strand at 5269.057 kb on + strand, within HSERO_RS22960 at 5269.186 kb on - strand at 5269.319 kb on - strand at 5269.319 kb on - strand at 5269.319 kb on - strand at 5269.330 kb on - strand at 5269.355 kb on - strand at 5269.559 kb on + strand, within HSERO_RS22965 at 5269.780 kb on - strand at 5269.810 kb on + strand at 5269.942 kb on - strand at 5269.969 kb on - strand, within HSERO_RS22970 at 5270.006 kb on - strand, within HSERO_RS22970 at 5270.055 kb on + strand, within HSERO_RS22970 at 5270.314 kb on - strand, within HSERO_RS22970 at 5270.648 kb on + strand, within HSERO_RS22970 at 5270.682 kb on + strand, within HSERO_RS22970 at 5270.703 kb on - strand, within HSERO_RS22970
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 5,268,632 + +0.3 5,268,759 - -1.9 5,268,791 - -1.1 5,269,057 + HSERO_RS22960 0.66 -0.2 5,269,186 - +0.3 5,269,319 - -1.4 5,269,319 - +0.1 5,269,319 - +0.3 5,269,330 - +0.3 5,269,355 - -0.4 5,269,559 + HSERO_RS22965 0.54 +0.6 5,269,780 - +0.6 5,269,810 + +0.7 5,269,942 - -2.6 5,269,969 - HSERO_RS22970 0.11 -0.1 5,270,006 - HSERO_RS22970 0.13 -2.7 5,270,055 + HSERO_RS22970 0.16 +0.9 5,270,314 - HSERO_RS22970 0.32 -0.8 5,270,648 + HSERO_RS22970 0.52 -0.2 5,270,682 + HSERO_RS22970 0.54 +0.9 5,270,703 - HSERO_RS22970 0.55 +0.6
Or see this region's nucleotide sequence