Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22520 and HSERO_RS22525 are separated by 235 nucleotides HSERO_RS22525 and HSERO_RS22530 are separated by 97 nucleotides HSERO_RS22530 and HSERO_RS22535 are separated by 319 nucleotides HSERO_RS22535 and HSERO_RS22540 are separated by 22 nucleotides
HSERO_RS22520: HSERO_RS22520 - hypothetical protein, at 5,166,437 to 5,167,012
_RS22520
HSERO_RS22525: HSERO_RS22525 - glyoxalase, at 5,167,248 to 5,167,625
_RS22525
HSERO_RS22530: HSERO_RS22530 - membrane protein, at 5,167,723 to 5,168,427
_RS22530
HSERO_RS22535: HSERO_RS22535 - LuxR family transcriptional regulator, at 5,168,747 to 5,169,367
_RS22535
HSERO_RS22540: HSERO_RS22540 - membrane protein, at 5,169,390 to 5,170,625
_RS22540
Position (kb) 5167
5168
5169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5166.798 kb on + strand, within HSERO_RS22520 at 5166.830 kb on - strand, within HSERO_RS22520 at 5166.832 kb on - strand, within HSERO_RS22520 at 5166.956 kb on - strand at 5166.970 kb on - strand at 5167.041 kb on + strand at 5167.046 kb on + strand at 5167.046 kb on + strand at 5167.049 kb on - strand at 5167.195 kb on + strand at 5167.407 kb on + strand, within HSERO_RS22525 at 5167.448 kb on - strand, within HSERO_RS22525 at 5167.657 kb on - strand at 5167.831 kb on + strand, within HSERO_RS22530 at 5167.872 kb on - strand, within HSERO_RS22530 at 5167.958 kb on - strand, within HSERO_RS22530 at 5168.097 kb on - strand, within HSERO_RS22530 at 5168.183 kb on - strand, within HSERO_RS22530 at 5168.264 kb on - strand, within HSERO_RS22530 at 5168.273 kb on + strand, within HSERO_RS22530 at 5168.331 kb on + strand at 5168.449 kb on - strand at 5168.456 kb on + strand at 5168.576 kb on + strand at 5168.584 kb on - strand at 5168.584 kb on - strand at 5168.622 kb on + strand at 5168.622 kb on + strand at 5168.630 kb on - strand at 5168.630 kb on - strand at 5168.630 kb on - strand at 5168.630 kb on - strand at 5168.785 kb on + strand at 5168.839 kb on - strand, within HSERO_RS22535 at 5168.846 kb on - strand, within HSERO_RS22535 at 5168.927 kb on - strand, within HSERO_RS22535 at 5169.010 kb on - strand, within HSERO_RS22535 at 5169.298 kb on + strand, within HSERO_RS22535 at 5169.306 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 5,166,798 + HSERO_RS22520 0.63 -0.8 5,166,830 - HSERO_RS22520 0.68 -3.6 5,166,832 - HSERO_RS22520 0.69 -1.1 5,166,956 - -1.3 5,166,970 - -0.6 5,167,041 + +0.6 5,167,046 + -0.5 5,167,046 + +0.7 5,167,049 - -0.3 5,167,195 + -0.1 5,167,407 + HSERO_RS22525 0.42 -0.4 5,167,448 - HSERO_RS22525 0.53 +1.3 5,167,657 - -0.9 5,167,831 + HSERO_RS22530 0.15 -2.0 5,167,872 - HSERO_RS22530 0.21 +0.7 5,167,958 - HSERO_RS22530 0.33 -0.4 5,168,097 - HSERO_RS22530 0.53 -2.2 5,168,183 - HSERO_RS22530 0.65 +2.0 5,168,264 - HSERO_RS22530 0.77 +0.3 5,168,273 + HSERO_RS22530 0.78 +1.4 5,168,331 + +0.8 5,168,449 - +1.6 5,168,456 + +0.0 5,168,576 + -0.0 5,168,584 - -0.1 5,168,584 - +0.8 5,168,622 + +0.1 5,168,622 + +0.3 5,168,630 - -0.6 5,168,630 - +1.4 5,168,630 - -0.2 5,168,630 - +0.1 5,168,785 + +0.4 5,168,839 - HSERO_RS22535 0.15 -0.2 5,168,846 - HSERO_RS22535 0.16 -0.6 5,168,927 - HSERO_RS22535 0.29 -0.2 5,169,010 - HSERO_RS22535 0.42 -1.5 5,169,298 + HSERO_RS22535 0.89 -0.1 5,169,306 - +0.9
Or see this region's nucleotide sequence