Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22250 and HSERO_RS22255 are separated by 80 nucleotides HSERO_RS22255 and HSERO_RS22260 overlap by 4 nucleotides HSERO_RS22260 and HSERO_RS22265 are separated by 37 nucleotides
HSERO_RS22250: HSERO_RS22250 - membrane protein, at 5,089,022 to 5,090,317
_RS22250
HSERO_RS22255: HSERO_RS22255 - 3-phosphoglycerate dehydrogenase, at 5,090,398 to 5,091,324
_RS22255
HSERO_RS22260: HSERO_RS22260 - diguanylate cyclase, at 5,091,321 to 5,092,010
_RS22260
HSERO_RS22265: HSERO_RS22265 - gluconolactonase, at 5,092,048 to 5,092,968
_RS22265
Position (kb) 5090
5091
5092 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5089.564 kb on - strand, within HSERO_RS22250 at 5089.662 kb on - strand, within HSERO_RS22250 at 5089.680 kb on - strand, within HSERO_RS22250 at 5089.680 kb on - strand, within HSERO_RS22250 at 5090.090 kb on + strand, within HSERO_RS22250 at 5090.239 kb on - strand at 5090.318 kb on - strand at 5090.398 kb on - strand at 5090.422 kb on - strand at 5090.638 kb on - strand, within HSERO_RS22255 at 5090.734 kb on - strand, within HSERO_RS22255 at 5090.773 kb on + strand, within HSERO_RS22255 at 5090.796 kb on + strand, within HSERO_RS22255 at 5090.803 kb on + strand, within HSERO_RS22255 at 5090.804 kb on - strand, within HSERO_RS22255 at 5090.804 kb on - strand, within HSERO_RS22255 at 5090.895 kb on + strand, within HSERO_RS22255 at 5090.942 kb on + strand, within HSERO_RS22255 at 5090.946 kb on + strand, within HSERO_RS22255 at 5090.977 kb on - strand, within HSERO_RS22255 at 5091.055 kb on - strand, within HSERO_RS22255 at 5091.172 kb on - strand, within HSERO_RS22255 at 5091.238 kb on - strand at 5091.702 kb on - strand, within HSERO_RS22260 at 5091.702 kb on - strand, within HSERO_RS22260 at 5092.013 kb on - strand at 5092.076 kb on + strand at 5092.084 kb on - strand at 5092.098 kb on + strand at 5092.171 kb on + strand, within HSERO_RS22265 at 5092.254 kb on - strand, within HSERO_RS22265
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 5,089,564 - HSERO_RS22250 0.42 +0.8 5,089,662 - HSERO_RS22250 0.49 -0.1 5,089,680 - HSERO_RS22250 0.51 -1.5 5,089,680 - HSERO_RS22250 0.51 -0.4 5,090,090 + HSERO_RS22250 0.82 +0.4 5,090,239 - -0.6 5,090,318 - -0.2 5,090,398 - -1.0 5,090,422 - -0.2 5,090,638 - HSERO_RS22255 0.26 +0.3 5,090,734 - HSERO_RS22255 0.36 +0.0 5,090,773 + HSERO_RS22255 0.40 +0.9 5,090,796 + HSERO_RS22255 0.43 -0.3 5,090,803 + HSERO_RS22255 0.44 -0.2 5,090,804 - HSERO_RS22255 0.44 +0.4 5,090,804 - HSERO_RS22255 0.44 +1.0 5,090,895 + HSERO_RS22255 0.54 -0.9 5,090,942 + HSERO_RS22255 0.59 -0.4 5,090,946 + HSERO_RS22255 0.59 +0.9 5,090,977 - HSERO_RS22255 0.62 -0.2 5,091,055 - HSERO_RS22255 0.71 -2.6 5,091,172 - HSERO_RS22255 0.83 +0.7 5,091,238 - +0.9 5,091,702 - HSERO_RS22260 0.55 -1.1 5,091,702 - HSERO_RS22260 0.55 +1.0 5,092,013 - +3.1 5,092,076 + -1.5 5,092,084 - +0.1 5,092,098 + +0.2 5,092,171 + HSERO_RS22265 0.13 +0.2 5,092,254 - HSERO_RS22265 0.22 -2.3
Or see this region's nucleotide sequence