Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22015 and HSERO_RS22020 are separated by 86 nucleotides HSERO_RS22020 and HSERO_RS22025 are separated by 19 nucleotides HSERO_RS22025 and HSERO_RS22030 are separated by 477 nucleotides
HSERO_RS22015: HSERO_RS22015 - DNA-binding protein, at 5,033,820 to 5,034,212
_RS22015
HSERO_RS22020: HSERO_RS22020 - membrane protein, at 5,034,299 to 5,034,904
_RS22020
HSERO_RS22025: HSERO_RS22025 - aromatic amino acid aminotransferase, at 5,034,924 to 5,036,114
_RS22025
HSERO_RS22030: HSERO_RS22030 - LysR family transcriptional regulator, at 5,036,592 to 5,037,515
_RS22030
Position (kb)
5034
5035
5036
5037 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5033.980 kb on + strand, within HSERO_RS22015 at 5034.099 kb on - strand, within HSERO_RS22015 at 5034.151 kb on - strand, within HSERO_RS22015 at 5034.167 kb on + strand, within HSERO_RS22015 at 5034.195 kb on + strand at 5034.236 kb on + strand at 5034.337 kb on - strand at 5034.385 kb on - strand, within HSERO_RS22020 at 5034.460 kb on + strand, within HSERO_RS22020 at 5034.509 kb on + strand, within HSERO_RS22020 at 5034.573 kb on + strand, within HSERO_RS22020 at 5034.578 kb on - strand, within HSERO_RS22020 at 5034.810 kb on + strand, within HSERO_RS22020 at 5035.064 kb on + strand, within HSERO_RS22025 at 5035.272 kb on - strand, within HSERO_RS22025 at 5035.441 kb on + strand, within HSERO_RS22025 at 5035.606 kb on + strand, within HSERO_RS22025 at 5035.662 kb on - strand, within HSERO_RS22025 at 5036.103 kb on - strand at 5036.214 kb on + strand at 5036.222 kb on - strand at 5036.245 kb on + strand at 5036.389 kb on + strand at 5036.399 kb on - strand at 5036.461 kb on + strand at 5036.507 kb on + strand at 5036.512 kb on + strand at 5036.605 kb on - strand at 5036.614 kb on + strand at 5036.622 kb on - strand at 5036.661 kb on + strand at 5036.661 kb on + strand at 5036.705 kb on + strand, within HSERO_RS22030 at 5036.752 kb on + strand, within HSERO_RS22030 at 5036.805 kb on + strand, within HSERO_RS22030 at 5036.820 kb on - strand, within HSERO_RS22030 at 5036.834 kb on + strand, within HSERO_RS22030 at 5036.871 kb on + strand, within HSERO_RS22030 at 5037.036 kb on - strand, within HSERO_RS22030 at 5037.036 kb on - strand, within HSERO_RS22030
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 5,033,980 + HSERO_RS22015 0.41 +0.7 5,034,099 - HSERO_RS22015 0.71 +0.4 5,034,151 - HSERO_RS22015 0.84 +0.8 5,034,167 + HSERO_RS22015 0.88 -2.0 5,034,195 + -1.2 5,034,236 + +0.6 5,034,337 - +1.0 5,034,385 - HSERO_RS22020 0.14 -0.7 5,034,460 + HSERO_RS22020 0.27 +0.8 5,034,509 + HSERO_RS22020 0.35 +0.8 5,034,573 + HSERO_RS22020 0.45 -0.6 5,034,578 - HSERO_RS22020 0.46 -2.9 5,034,810 + HSERO_RS22020 0.84 -1.8 5,035,064 + HSERO_RS22025 0.12 -0.2 5,035,272 - HSERO_RS22025 0.29 -1.5 5,035,441 + HSERO_RS22025 0.43 -0.5 5,035,606 + HSERO_RS22025 0.57 -1.3 5,035,662 - HSERO_RS22025 0.62 -0.6 5,036,103 - -0.4 5,036,214 + +0.5 5,036,222 - -3.1 5,036,245 + -1.5 5,036,389 + -0.5 5,036,399 - -1.7 5,036,461 + -1.4 5,036,507 + -0.1 5,036,512 + -0.6 5,036,605 - +0.7 5,036,614 + -0.1 5,036,622 - -1.1 5,036,661 + +1.8 5,036,661 + -0.3 5,036,705 + HSERO_RS22030 0.12 +0.4 5,036,752 + HSERO_RS22030 0.17 +1.1 5,036,805 + HSERO_RS22030 0.23 +0.2 5,036,820 - HSERO_RS22030 0.25 +0.1 5,036,834 + HSERO_RS22030 0.26 -0.2 5,036,871 + HSERO_RS22030 0.30 +0.7 5,037,036 - HSERO_RS22030 0.48 -2.5 5,037,036 - HSERO_RS22030 0.48 +1.4
Or see this region's nucleotide sequence