Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22000 and HSERO_RS22005 are separated by 17 nucleotides HSERO_RS22005 and HSERO_RS22010 are separated by 126 nucleotides HSERO_RS22010 and HSERO_RS22015 are separated by 17 nucleotides HSERO_RS22015 and HSERO_RS22020 are separated by 86 nucleotides
HSERO_RS22000: HSERO_RS22000 - short-chain dehydrogenase, at 5,032,202 to 5,032,969
_RS22000
HSERO_RS22005: HSERO_RS22005 - diguanylate cyclase, at 5,032,987 to 5,033,382
_RS22005
HSERO_RS22010: HSERO_RS22010 - toxin RelE, at 5,033,509 to 5,033,802
_RS22010
HSERO_RS22015: HSERO_RS22015 - DNA-binding protein, at 5,033,820 to 5,034,212
_RS22015
HSERO_RS22020: HSERO_RS22020 - membrane protein, at 5,034,299 to 5,034,904
_RS22020
Position (kb) 5033
5034 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5032.665 kb on + strand, within HSERO_RS22000 at 5032.673 kb on - strand, within HSERO_RS22000 at 5032.685 kb on + strand, within HSERO_RS22000 at 5032.828 kb on - strand, within HSERO_RS22000 at 5032.841 kb on + strand at 5032.849 kb on - strand, within HSERO_RS22000 at 5033.466 kb on + strand at 5033.575 kb on - strand, within HSERO_RS22010 at 5033.603 kb on - strand, within HSERO_RS22010 at 5033.693 kb on - strand, within HSERO_RS22010 at 5033.980 kb on + strand, within HSERO_RS22015 at 5034.099 kb on - strand, within HSERO_RS22015 at 5034.151 kb on - strand, within HSERO_RS22015 at 5034.167 kb on + strand, within HSERO_RS22015 at 5034.195 kb on + strand at 5034.236 kb on + strand at 5034.337 kb on - strand at 5034.385 kb on - strand, within HSERO_RS22020 at 5034.460 kb on + strand, within HSERO_RS22020 at 5034.509 kb on + strand, within HSERO_RS22020 at 5034.573 kb on + strand, within HSERO_RS22020 at 5034.578 kb on - strand, within HSERO_RS22020
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 5,032,665 + HSERO_RS22000 0.60 +0.1 5,032,673 - HSERO_RS22000 0.61 -2.5 5,032,685 + HSERO_RS22000 0.63 -1.0 5,032,828 - HSERO_RS22000 0.82 -2.2 5,032,841 + +2.8 5,032,849 - HSERO_RS22000 0.84 -1.1 5,033,466 + -0.7 5,033,575 - HSERO_RS22010 0.22 +0.9 5,033,603 - HSERO_RS22010 0.32 +0.0 5,033,693 - HSERO_RS22010 0.63 +0.3 5,033,980 + HSERO_RS22015 0.41 +0.7 5,034,099 - HSERO_RS22015 0.71 +0.4 5,034,151 - HSERO_RS22015 0.84 +0.8 5,034,167 + HSERO_RS22015 0.88 -2.0 5,034,195 + -1.2 5,034,236 + +0.6 5,034,337 - +1.0 5,034,385 - HSERO_RS22020 0.14 -0.7 5,034,460 + HSERO_RS22020 0.27 +0.8 5,034,509 + HSERO_RS22020 0.35 +0.8 5,034,573 + HSERO_RS22020 0.45 -0.6 5,034,578 - HSERO_RS22020 0.46 -2.9
Or see this region's nucleotide sequence