Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19415 and HSERO_RS19420 are separated by 186 nucleotides HSERO_RS19420 and HSERO_RS19425 are separated by 209 nucleotides HSERO_RS19425 and HSERO_RS19430 are separated by 44 nucleotides
HSERO_RS19415: HSERO_RS19415 - membrane protein, at 4,450,306 to 4,451,538
_RS19415
HSERO_RS19420: HSERO_RS19420 - cobalt transporter, at 4,451,725 to 4,452,099
_RS19420
HSERO_RS19425: HSERO_RS19425 - ribulose-phosphate 3-epimerase, at 4,452,309 to 4,452,974
_RS19425
HSERO_RS19430: HSERO_RS19430 - phosphoglycolate phosphatase, at 4,453,019 to 4,453,699
_RS19430
Position (kb) 4452
4453 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4451.324 kb on + strand, within HSERO_RS19415 at 4451.532 kb on + strand at 4452.995 kb on - strand at 4452.995 kb on - strand at 4453.308 kb on + strand, within HSERO_RS19430 at 4453.316 kb on - strand, within HSERO_RS19430 at 4453.326 kb on - strand, within HSERO_RS19430 at 4453.363 kb on - strand, within HSERO_RS19430 at 4453.393 kb on - strand, within HSERO_RS19430 at 4453.408 kb on + strand at 4453.410 kb on + strand, within HSERO_RS19430 at 4453.418 kb on - strand, within HSERO_RS19430 at 4453.497 kb on - strand, within HSERO_RS19430 at 4453.625 kb on + strand, within HSERO_RS19430 at 4453.650 kb on + strand at 4453.650 kb on + strand at 4453.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 4,451,324 + HSERO_RS19415 0.83 -1.2 4,451,532 + +3.2 4,452,995 - +1.0 4,452,995 - +1.0 4,453,308 + HSERO_RS19430 0.42 +0.4 4,453,316 - HSERO_RS19430 0.44 -0.1 4,453,326 - HSERO_RS19430 0.45 +1.4 4,453,363 - HSERO_RS19430 0.51 -2.6 4,453,393 - HSERO_RS19430 0.55 +1.2 4,453,408 + -1.0 4,453,410 + HSERO_RS19430 0.57 +0.2 4,453,418 - HSERO_RS19430 0.59 +0.6 4,453,497 - HSERO_RS19430 0.70 +0.2 4,453,625 + HSERO_RS19430 0.89 -0.3 4,453,650 + +1.5 4,453,650 + +1.2 4,453,805 - +0.3
Or see this region's nucleotide sequence