Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19405 and HSERO_RS19410 are separated by 218 nucleotides HSERO_RS19410 and HSERO_RS19415 are separated by 82 nucleotides HSERO_RS19415 and HSERO_RS19420 are separated by 186 nucleotides HSERO_RS19420 and HSERO_RS19425 are separated by 209 nucleotides
HSERO_RS19405: HSERO_RS19405 - enoyl-CoA hydratase, at 4,448,791 to 4,449,567
_RS19405
HSERO_RS19410: HSERO_RS19410 - universal stress protein, at 4,449,786 to 4,450,223
_RS19410
HSERO_RS19415: HSERO_RS19415 - membrane protein, at 4,450,306 to 4,451,538
_RS19415
HSERO_RS19420: HSERO_RS19420 - cobalt transporter, at 4,451,725 to 4,452,099
_RS19420
HSERO_RS19425: HSERO_RS19425 - ribulose-phosphate 3-epimerase, at 4,452,309 to 4,452,974
_RS19425
Position (kb) 4450
4451
4452 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4449.334 kb on - strand, within HSERO_RS19405 at 4449.334 kb on - strand, within HSERO_RS19405 at 4449.426 kb on + strand, within HSERO_RS19405 at 4449.562 kb on + strand at 4449.570 kb on - strand at 4449.575 kb on + strand at 4449.738 kb on - strand at 4449.788 kb on + strand at 4449.825 kb on + strand at 4449.849 kb on + strand, within HSERO_RS19410 at 4449.889 kb on + strand, within HSERO_RS19410 at 4449.905 kb on + strand, within HSERO_RS19410 at 4449.906 kb on + strand, within HSERO_RS19410 at 4449.926 kb on + strand at 4450.103 kb on + strand, within HSERO_RS19410 at 4450.171 kb on - strand, within HSERO_RS19410 at 4450.220 kb on - strand at 4450.222 kb on + strand at 4450.240 kb on - strand at 4450.313 kb on + strand at 4450.421 kb on + strand at 4450.650 kb on + strand at 4450.673 kb on - strand, within HSERO_RS19415 at 4450.729 kb on - strand, within HSERO_RS19415 at 4450.745 kb on - strand, within HSERO_RS19415 at 4450.781 kb on + strand at 4450.796 kb on + strand, within HSERO_RS19415 at 4450.796 kb on + strand, within HSERO_RS19415 at 4450.841 kb on - strand, within HSERO_RS19415 at 4450.922 kb on + strand, within HSERO_RS19415 at 4451.061 kb on - strand, within HSERO_RS19415 at 4451.061 kb on - strand, within HSERO_RS19415 at 4451.165 kb on + strand, within HSERO_RS19415 at 4451.308 kb on - strand, within HSERO_RS19415 at 4451.324 kb on + strand, within HSERO_RS19415 at 4451.532 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 4,449,334 - HSERO_RS19405 0.70 -0.4 4,449,334 - HSERO_RS19405 0.70 +0.1 4,449,426 + HSERO_RS19405 0.82 -0.6 4,449,562 + +0.2 4,449,570 - +0.8 4,449,575 + +1.2 4,449,738 - +0.4 4,449,788 + +0.6 4,449,825 + -0.9 4,449,849 + HSERO_RS19410 0.14 -0.9 4,449,889 + HSERO_RS19410 0.24 -0.6 4,449,905 + HSERO_RS19410 0.27 +0.5 4,449,906 + HSERO_RS19410 0.27 -0.2 4,449,926 + +1.6 4,450,103 + HSERO_RS19410 0.72 -3.0 4,450,171 - HSERO_RS19410 0.88 -0.6 4,450,220 - +0.0 4,450,222 + +0.4 4,450,240 - +0.1 4,450,313 + -0.8 4,450,421 + -2.1 4,450,650 + -0.4 4,450,673 - HSERO_RS19415 0.30 +0.8 4,450,729 - HSERO_RS19415 0.34 -2.1 4,450,745 - HSERO_RS19415 0.36 +0.2 4,450,781 + -0.4 4,450,796 + HSERO_RS19415 0.40 -0.1 4,450,796 + HSERO_RS19415 0.40 -2.6 4,450,841 - HSERO_RS19415 0.43 -1.3 4,450,922 + HSERO_RS19415 0.50 -0.3 4,451,061 - HSERO_RS19415 0.61 -1.1 4,451,061 - HSERO_RS19415 0.61 +0.1 4,451,165 + HSERO_RS19415 0.70 -1.8 4,451,308 - HSERO_RS19415 0.81 -1.0 4,451,324 + HSERO_RS19415 0.83 -1.2 4,451,532 + +3.2
Or see this region's nucleotide sequence