Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19130 and HSERO_RS19135 overlap by 4 nucleotides HSERO_RS19135 and HSERO_RS19140 are separated by 330 nucleotides HSERO_RS19140 and HSERO_RS19145 are separated by 215 nucleotides
HSERO_RS19130: HSERO_RS19130 - geranyl transferase, at 4,390,150 to 4,391,097
_RS19130
HSERO_RS19135: HSERO_RS19135 - exodeoxyribonuclease, at 4,391,094 to 4,391,321
_RS19135
HSERO_RS19140: HSERO_RS19140 - Rieske (2Fe-2S) protein, at 4,391,652 to 4,392,776
_RS19140
HSERO_RS19145: HSERO_RS19145 - drug/metabolite transporter (DMT) superfamily permease, at 4,392,992 to 4,393,909
_RS19145
Position (kb)
4391
4392
4393 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4391.139 kb on - strand, within HSERO_RS19135 at 4391.370 kb on - strand at 4391.417 kb on + strand at 4391.483 kb on - strand at 4391.553 kb on + strand at 4391.563 kb on - strand at 4391.565 kb on + strand at 4391.700 kb on + strand at 4391.732 kb on - strand at 4392.035 kb on + strand, within HSERO_RS19140 at 4392.251 kb on - strand, within HSERO_RS19140 at 4392.310 kb on + strand, within HSERO_RS19140 at 4393.011 kb on - strand at 4393.192 kb on - strand, within HSERO_RS19145 at 4393.230 kb on - strand at 4393.372 kb on - strand, within HSERO_RS19145 at 4393.475 kb on - strand, within HSERO_RS19145 at 4393.481 kb on - strand, within HSERO_RS19145 at 4393.561 kb on + strand, within HSERO_RS19145 at 4393.702 kb on + strand, within HSERO_RS19145
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 4,391,139 - HSERO_RS19135 0.20 -0.3 4,391,370 - -0.6 4,391,417 + +1.4 4,391,483 - -0.4 4,391,553 + +0.5 4,391,563 - -0.0 4,391,565 + +1.1 4,391,700 + +2.4 4,391,732 - -0.3 4,392,035 + HSERO_RS19140 0.34 +1.7 4,392,251 - HSERO_RS19140 0.53 -2.2 4,392,310 + HSERO_RS19140 0.58 +0.3 4,393,011 - +1.0 4,393,192 - HSERO_RS19145 0.22 -0.4 4,393,230 - -1.0 4,393,372 - HSERO_RS19145 0.41 +0.1 4,393,475 - HSERO_RS19145 0.53 -2.5 4,393,481 - HSERO_RS19145 0.53 -0.6 4,393,561 + HSERO_RS19145 0.62 -0.3 4,393,702 + HSERO_RS19145 0.77 -0.4
Or see this region's nucleotide sequence