Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS15385 and HSERO_RS15390 are separated by 77 nucleotides HSERO_RS15390 and HSERO_RS15395 overlap by 4 nucleotides HSERO_RS15395 and HSERO_RS15400 are separated by 75 nucleotides
HSERO_RS15385: HSERO_RS15385 - hypothetical protein, at 3,521,230 to 3,522,204
_RS15385
HSERO_RS15390: HSERO_RS15390 - 3-hydroxyisobutyrate dehydrogenase, at 3,522,282 to 3,523,175
_RS15390
HSERO_RS15395: HSERO_RS15395 - methylmalonate-semialdehyde dehydrogenase, at 3,523,172 to 3,524,692
_RS15395
HSERO_RS15400: HSERO_RS15400 - ABC transporter permease, at 3,524,768 to 3,526,000
_RS15400
Position (kb)
3523
3524
3525 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3522.279 kb on + strand at 3522.505 kb on + strand, within HSERO_RS15390 at 3522.619 kb on + strand, within HSERO_RS15390 at 3522.642 kb on - strand, within HSERO_RS15390 at 3523.069 kb on + strand at 3523.123 kb on - strand at 3523.192 kb on - strand at 3523.493 kb on - strand, within HSERO_RS15395 at 3523.709 kb on - strand, within HSERO_RS15395 at 3523.769 kb on - strand, within HSERO_RS15395 at 3523.798 kb on - strand, within HSERO_RS15395 at 3524.064 kb on - strand, within HSERO_RS15395 at 3524.183 kb on + strand, within HSERO_RS15395 at 3524.258 kb on - strand, within HSERO_RS15395 at 3524.356 kb on + strand, within HSERO_RS15395 at 3524.499 kb on + strand, within HSERO_RS15395 at 3524.511 kb on + strand, within HSERO_RS15395 at 3524.587 kb on + strand at 3524.603 kb on + strand at 3524.706 kb on + strand at 3524.713 kb on + strand at 3524.721 kb on - strand at 3524.859 kb on + strand at 3524.877 kb on + strand at 3524.972 kb on + strand, within HSERO_RS15400 at 3524.978 kb on - strand, within HSERO_RS15400 at 3525.089 kb on - strand, within HSERO_RS15400 at 3525.089 kb on - strand, within HSERO_RS15400 at 3525.161 kb on - strand, within HSERO_RS15400 at 3525.429 kb on + strand, within HSERO_RS15400
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 3,522,279 + +0.6 3,522,505 + HSERO_RS15390 0.25 -1.3 3,522,619 + HSERO_RS15390 0.38 +0.6 3,522,642 - HSERO_RS15390 0.40 +0.9 3,523,069 + -0.4 3,523,123 - +0.2 3,523,192 - -0.6 3,523,493 - HSERO_RS15395 0.21 +0.8 3,523,709 - HSERO_RS15395 0.35 -0.7 3,523,769 - HSERO_RS15395 0.39 +0.9 3,523,798 - HSERO_RS15395 0.41 -0.0 3,524,064 - HSERO_RS15395 0.59 +2.7 3,524,183 + HSERO_RS15395 0.66 -1.1 3,524,258 - HSERO_RS15395 0.71 +3.0 3,524,356 + HSERO_RS15395 0.78 -0.7 3,524,499 + HSERO_RS15395 0.87 +0.1 3,524,511 + HSERO_RS15395 0.88 -0.6 3,524,587 + -0.7 3,524,603 + -1.9 3,524,706 + -1.3 3,524,713 + -1.9 3,524,721 - +0.0 3,524,859 + -0.0 3,524,877 + +1.5 3,524,972 + HSERO_RS15400 0.17 -1.0 3,524,978 - HSERO_RS15400 0.17 +0.3 3,525,089 - HSERO_RS15400 0.26 +1.8 3,525,089 - HSERO_RS15400 0.26 +0.1 3,525,161 - HSERO_RS15400 0.32 -1.1 3,525,429 + HSERO_RS15400 0.54 +1.1
Or see this region's nucleotide sequence