Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12010 and HSERO_RS12015 are separated by 227 nucleotides HSERO_RS12015 and HSERO_RS12020 are separated by 38 nucleotides HSERO_RS12020 and HSERO_RS12025 are separated by 1 nucleotides HSERO_RS12025 and HSERO_RS12030 are separated by 54 nucleotides
HSERO_RS12010: HSERO_RS12010 - hemerythrin, at 2,731,393 to 2,731,800
_RS12010
HSERO_RS12015: HSERO_RS12015 - molecular chaperone DnaK, at 2,732,028 to 2,732,360
_RS12015
HSERO_RS12020: HSERO_RS12020 - poly-beta-hydroxybutyrate polymerase, at 2,732,399 to 2,734,129
_RS12020
HSERO_RS12025: HSERO_RS12025 - enoyl-ACP reductase, at 2,734,131 to 2,734,898
_RS12025
HSERO_RS12030: HSERO_RS12030 - phosphate acetyl/butaryl transferase, at 2,734,953 to 2,736,407
_RS12030
Position (kb)
2732
2733
2734
2735 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2731.627 kb on + strand, within HSERO_RS12010 at 2731.676 kb on - strand, within HSERO_RS12010 at 2731.818 kb on + strand at 2731.841 kb on - strand at 2731.841 kb on - strand at 2731.841 kb on - strand at 2731.925 kb on - strand at 2732.044 kb on - strand at 2732.068 kb on - strand, within HSERO_RS12015 at 2732.457 kb on - strand at 2732.480 kb on - strand at 2732.501 kb on - strand at 2732.537 kb on - strand at 2732.563 kb on + strand at 2732.630 kb on - strand, within HSERO_RS12020 at 2732.711 kb on + strand, within HSERO_RS12020 at 2732.856 kb on + strand at 2732.856 kb on + strand, within HSERO_RS12020 at 2732.977 kb on + strand, within HSERO_RS12020 at 2733.063 kb on + strand, within HSERO_RS12020 at 2733.118 kb on + strand, within HSERO_RS12020 at 2733.177 kb on + strand, within HSERO_RS12020 at 2733.325 kb on - strand, within HSERO_RS12020 at 2733.560 kb on - strand, within HSERO_RS12020 at 2733.677 kb on - strand, within HSERO_RS12020 at 2734.036 kb on + strand at 2734.090 kb on - strand at 2734.097 kb on - strand at 2734.184 kb on + strand at 2734.194 kb on + strand at 2734.202 kb on - strand at 2734.271 kb on - strand, within HSERO_RS12025 at 2734.411 kb on + strand, within HSERO_RS12025 at 2734.423 kb on + strand, within HSERO_RS12025 at 2734.613 kb on + strand, within HSERO_RS12025 at 2734.952 kb on - strand at 2735.083 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 2,731,627 + HSERO_RS12010 0.57 -0.8 2,731,676 - HSERO_RS12010 0.69 -0.6 2,731,818 + -0.0 2,731,841 - +0.8 2,731,841 - +1.3 2,731,841 - +0.1 2,731,925 - +0.0 2,732,044 - +1.9 2,732,068 - HSERO_RS12015 0.12 -0.3 2,732,457 - -0.5 2,732,480 - +0.8 2,732,501 - -0.3 2,732,537 - +0.4 2,732,563 + -0.3 2,732,630 - HSERO_RS12020 0.13 -0.7 2,732,711 + HSERO_RS12020 0.18 -0.4 2,732,856 + +1.6 2,732,856 + HSERO_RS12020 0.26 -0.6 2,732,977 + HSERO_RS12020 0.33 +0.6 2,733,063 + HSERO_RS12020 0.38 +0.5 2,733,118 + HSERO_RS12020 0.42 +0.0 2,733,177 + HSERO_RS12020 0.45 +1.2 2,733,325 - HSERO_RS12020 0.53 +0.8 2,733,560 - HSERO_RS12020 0.67 +1.8 2,733,677 - HSERO_RS12020 0.74 +1.9 2,734,036 + +0.6 2,734,090 - +0.6 2,734,097 - -1.0 2,734,184 + +1.9 2,734,194 + -2.3 2,734,202 - +0.4 2,734,271 - HSERO_RS12025 0.18 +0.6 2,734,411 + HSERO_RS12025 0.36 +0.6 2,734,423 + HSERO_RS12025 0.38 +0.9 2,734,613 + HSERO_RS12025 0.63 -0.5 2,734,952 - -0.7 2,735,083 + -0.6
Or see this region's nucleotide sequence