Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS09290 and HSERO_RS09295 are separated by 110 nucleotides HSERO_RS09295 and HSERO_RS09300 are separated by 124 nucleotides HSERO_RS09300 and HSERO_RS09305 are separated by 55 nucleotides HSERO_RS09305 and HSERO_RS09310 are separated by 30 nucleotides
HSERO_RS09290: HSERO_RS09290 - ribonuclease R, at 2,111,696 to 2,114,215
_RS09290
HSERO_RS09295: HSERO_RS09295 - 23S rRNA (guanosine-2'-O-)-methyltransferase, at 2,114,326 to 2,115,063
_RS09295
HSERO_RS09300: HSERO_RS09300 - serine O-acetyltransferase, at 2,115,188 to 2,115,940
_RS09300
HSERO_RS09305: HSERO_RS09305 - UDP-2,3-diacylglucosamine hydrolase, at 2,115,996 to 2,116,787
_RS09305
HSERO_RS09310: HSERO_RS09310 - cyclophilin, at 2,116,818 to 2,117,306
_RS09310
Position (kb)
2115
2116 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2114.521 kb on + strand, within HSERO_RS09295 at 2114.535 kb on - strand, within HSERO_RS09295 at 2114.683 kb on + strand, within HSERO_RS09295 at 2114.730 kb on - strand, within HSERO_RS09295 at 2114.813 kb on + strand, within HSERO_RS09295 at 2114.978 kb on + strand, within HSERO_RS09295 at 2115.196 kb on - strand at 2115.207 kb on - strand at 2115.242 kb on + strand at 2115.316 kb on - strand at 2115.395 kb on - strand, within HSERO_RS09300 at 2115.405 kb on + strand, within HSERO_RS09300 at 2115.443 kb on - strand at 2115.650 kb on - strand, within HSERO_RS09300 at 2115.676 kb on - strand, within HSERO_RS09300 at 2115.714 kb on - strand, within HSERO_RS09300 at 2115.982 kb on + strand at 2115.990 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 2,114,521 + HSERO_RS09295 0.26 -1.5 2,114,535 - HSERO_RS09295 0.28 -1.4 2,114,683 + HSERO_RS09295 0.48 -2.5 2,114,730 - HSERO_RS09295 0.55 -0.8 2,114,813 + HSERO_RS09295 0.66 +1.8 2,114,978 + HSERO_RS09295 0.88 -0.8 2,115,196 - -0.8 2,115,207 - +0.3 2,115,242 + +1.6 2,115,316 - -0.7 2,115,395 - HSERO_RS09300 0.27 +0.1 2,115,405 + HSERO_RS09300 0.29 +0.1 2,115,443 - -0.0 2,115,650 - HSERO_RS09300 0.61 -0.0 2,115,676 - HSERO_RS09300 0.65 +0.5 2,115,714 - HSERO_RS09300 0.70 +0.1 2,115,982 + -1.1 2,115,990 - -0.0
Or see this region's nucleotide sequence