Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05240 and HSERO_RS05245 are separated by 7 nucleotides HSERO_RS05245 and HSERO_RS05250 are separated by 158 nucleotides HSERO_RS05250 and HSERO_RS05255 are separated by 37 nucleotides
HSERO_RS05240: HSERO_RS05240 - short-chain dehydrogenase, at 1,160,755 to 1,161,531
_RS05240
HSERO_RS05245: HSERO_RS05245 - hypothetical protein, at 1,161,539 to 1,161,901
_RS05245
HSERO_RS05250: HSERO_RS05250 - ABC transporter for L-fucose, ATPase component (from data) , at 1,162,060 to 1,163,622
_RS05250
HSERO_RS05255: HSERO_RS05255 - ABC transporter for L-fucose, permease component (from data) , at 1,163,660 to 1,164,703
_RS05255
Position (kb)
1162
1163
1164 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1161.115 kb on + strand, within HSERO_RS05240 at 1161.234 kb on + strand, within HSERO_RS05240 at 1161.245 kb on + strand, within HSERO_RS05240 at 1161.411 kb on - strand, within HSERO_RS05240 at 1161.719 kb on + strand, within HSERO_RS05245 at 1161.782 kb on + strand, within HSERO_RS05245 at 1161.850 kb on - strand, within HSERO_RS05245 at 1161.976 kb on + strand at 1162.054 kb on - strand at 1162.445 kb on - strand, within HSERO_RS05250 at 1162.586 kb on + strand, within HSERO_RS05250 at 1162.613 kb on - strand, within HSERO_RS05250 at 1162.694 kb on - strand, within HSERO_RS05250 at 1162.698 kb on + strand, within HSERO_RS05250 at 1162.715 kb on + strand, within HSERO_RS05250 at 1162.889 kb on + strand, within HSERO_RS05250 at 1162.960 kb on + strand, within HSERO_RS05250 at 1163.049 kb on - strand, within HSERO_RS05250 at 1163.171 kb on + strand, within HSERO_RS05250 at 1163.174 kb on - strand, within HSERO_RS05250 at 1163.459 kb on - strand, within HSERO_RS05250 at 1163.459 kb on - strand, within HSERO_RS05250 at 1163.474 kb on + strand at 1163.721 kb on + strand at 1163.729 kb on - strand at 1163.776 kb on - strand, within HSERO_RS05255 at 1163.834 kb on + strand, within HSERO_RS05255 at 1163.944 kb on - strand, within HSERO_RS05255 at 1164.084 kb on - strand, within HSERO_RS05255 at 1164.121 kb on + strand at 1164.129 kb on - strand, within HSERO_RS05255 at 1164.213 kb on + strand, within HSERO_RS05255 at 1164.334 kb on - strand, within HSERO_RS05255 at 1164.336 kb on + strand, within HSERO_RS05255 at 1164.582 kb on - strand, within HSERO_RS05255
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 1,161,115 + HSERO_RS05240 0.46 +0.1 1,161,234 + HSERO_RS05240 0.62 +0.9 1,161,245 + HSERO_RS05240 0.63 -0.5 1,161,411 - HSERO_RS05240 0.84 +0.9 1,161,719 + HSERO_RS05245 0.50 -0.1 1,161,782 + HSERO_RS05245 0.67 +0.2 1,161,850 - HSERO_RS05245 0.86 -0.2 1,161,976 + +0.0 1,162,054 - -0.6 1,162,445 - HSERO_RS05250 0.25 -1.4 1,162,586 + HSERO_RS05250 0.34 -0.2 1,162,613 - HSERO_RS05250 0.35 -0.8 1,162,694 - HSERO_RS05250 0.41 +0.4 1,162,698 + HSERO_RS05250 0.41 -1.5 1,162,715 + HSERO_RS05250 0.42 +1.6 1,162,889 + HSERO_RS05250 0.53 +0.8 1,162,960 + HSERO_RS05250 0.58 -0.2 1,163,049 - HSERO_RS05250 0.63 -0.1 1,163,171 + HSERO_RS05250 0.71 -0.8 1,163,174 - HSERO_RS05250 0.71 +0.0 1,163,459 - HSERO_RS05250 0.90 +0.0 1,163,459 - HSERO_RS05250 0.90 -3.7 1,163,474 + +0.2 1,163,721 + -1.2 1,163,729 - +1.4 1,163,776 - HSERO_RS05255 0.11 -2.3 1,163,834 + HSERO_RS05255 0.17 +1.3 1,163,944 - HSERO_RS05255 0.27 +0.0 1,164,084 - HSERO_RS05255 0.41 +0.1 1,164,121 + +0.0 1,164,129 - HSERO_RS05255 0.45 -0.1 1,164,213 + HSERO_RS05255 0.53 -2.6 1,164,334 - HSERO_RS05255 0.65 -1.6 1,164,336 + HSERO_RS05255 0.65 -0.7 1,164,582 - HSERO_RS05255 0.88 +1.0
Or see this region's nucleotide sequence