Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03540 and HSERO_RS03545 are separated by 197 nucleotides HSERO_RS03545 and HSERO_RS03550 are separated by 201 nucleotides HSERO_RS03550 and HSERO_RS03555 are separated by 104 nucleotides
HSERO_RS03540: HSERO_RS03540 - dimethyladenosine transferase, at 771,352 to 772,134
_RS03540
HSERO_RS03545: HSERO_RS03545 - hypothetical protein, at 772,332 to 772,709
_RS03545
HSERO_RS03550: HSERO_RS03550 - glyoxalase I, at 772,911 to 773,315
_RS03550
HSERO_RS03555: HSERO_RS03555 - chloride channel protein, at 773,420 to 774,703
_RS03555
Position (kb) 772
773
774 Strain fitness (log2 ratio) -2
-1
0
1 at 771.951 kb on + strand, within HSERO_RS03540 at 772.250 kb on + strand at 772.304 kb on - strand at 772.397 kb on - strand, within HSERO_RS03545 at 772.413 kb on + strand, within HSERO_RS03545 at 772.557 kb on + strand, within HSERO_RS03545 at 772.656 kb on - strand, within HSERO_RS03545 at 772.746 kb on - strand at 772.773 kb on - strand at 772.793 kb on + strand at 772.794 kb on + strand at 772.802 kb on - strand at 772.980 kb on - strand, within HSERO_RS03550 at 772.992 kb on - strand, within HSERO_RS03550 at 773.318 kb on - strand at 773.835 kb on + strand, within HSERO_RS03555 at 773.977 kb on - strand, within HSERO_RS03555 at 774.175 kb on + strand, within HSERO_RS03555
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 771,951 + HSERO_RS03540 0.77 +0.4 772,250 + -2.5 772,304 - -0.4 772,397 - HSERO_RS03545 0.17 +0.7 772,413 + HSERO_RS03545 0.21 +0.3 772,557 + HSERO_RS03545 0.60 -1.1 772,656 - HSERO_RS03545 0.86 -1.6 772,746 - -0.8 772,773 - -0.6 772,793 + -2.0 772,794 + -0.3 772,802 - -1.2 772,980 - HSERO_RS03550 0.17 +0.4 772,992 - HSERO_RS03550 0.20 -1.3 773,318 - -0.4 773,835 + HSERO_RS03555 0.32 -0.6 773,977 - HSERO_RS03555 0.43 -0.1 774,175 + HSERO_RS03555 0.59 -1.7
Or see this region's nucleotide sequence