Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03335 and HSERO_RS03340 overlap by 4 nucleotides HSERO_RS03340 and HSERO_RS03345 are separated by 122 nucleotides HSERO_RS03345 and HSERO_RS03350 are separated by 227 nucleotides
HSERO_RS03335: HSERO_RS03335 - branched-chain amino acid ABC transporter permease, at 731,951 to 732,736
_RS03335
HSERO_RS03340: HSERO_RS03340 - branched-chain amino acid transporter, at 732,733 to 733,056
_RS03340
HSERO_RS03345: HSERO_RS03345 - phosphoglycerate kinase, at 733,179 to 734,369
_RS03345
HSERO_RS03350: HSERO_RS03350 - hypothetical protein, at 734,597 to 736,030
_RS03350
Position (kb) 733
734
735 Strain fitness (log2 ratio) -2
-1
0
1 at 732.208 kb on + strand, within HSERO_RS03335 at 732.299 kb on + strand, within HSERO_RS03335 at 732.324 kb on + strand, within HSERO_RS03335 at 732.349 kb on + strand, within HSERO_RS03335 at 732.554 kb on + strand, within HSERO_RS03335 at 732.580 kb on + strand, within HSERO_RS03335 at 732.580 kb on + strand, within HSERO_RS03335 at 732.593 kb on - strand, within HSERO_RS03335 at 732.593 kb on - strand, within HSERO_RS03335 at 732.636 kb on - strand, within HSERO_RS03335 at 732.683 kb on - strand at 732.776 kb on + strand, within HSERO_RS03340 at 732.789 kb on - strand, within HSERO_RS03340 at 732.863 kb on - strand, within HSERO_RS03340 at 732.893 kb on + strand, within HSERO_RS03340 at 732.911 kb on - strand, within HSERO_RS03340 at 732.934 kb on - strand, within HSERO_RS03340 at 733.036 kb on + strand at 733.124 kb on + strand at 733.124 kb on + strand at 733.124 kb on + strand at 733.132 kb on - strand at 734.553 kb on + strand at 734.851 kb on + strand, within HSERO_RS03350 at 734.984 kb on + strand, within HSERO_RS03350 at 735.100 kb on + strand, within HSERO_RS03350 at 735.142 kb on + strand, within HSERO_RS03350 at 735.318 kb on + strand, within HSERO_RS03350
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 732,208 + HSERO_RS03335 0.33 -2.0 732,299 + HSERO_RS03335 0.44 -1.8 732,324 + HSERO_RS03335 0.47 -2.6 732,349 + HSERO_RS03335 0.51 -0.3 732,554 + HSERO_RS03335 0.77 -1.5 732,580 + HSERO_RS03335 0.80 -1.1 732,580 + HSERO_RS03335 0.80 -0.2 732,593 - HSERO_RS03335 0.82 -0.1 732,593 - HSERO_RS03335 0.82 +0.2 732,636 - HSERO_RS03335 0.87 +0.3 732,683 - -1.7 732,776 + HSERO_RS03340 0.13 +0.6 732,789 - HSERO_RS03340 0.17 +0.8 732,863 - HSERO_RS03340 0.40 -0.0 732,893 + HSERO_RS03340 0.49 -2.6 732,911 - HSERO_RS03340 0.55 +0.8 732,934 - HSERO_RS03340 0.62 +0.3 733,036 + +0.6 733,124 + -0.4 733,124 + -1.3 733,124 + -0.8 733,132 - -1.2 734,553 + +1.3 734,851 + HSERO_RS03350 0.18 -2.1 734,984 + HSERO_RS03350 0.27 -0.7 735,100 + HSERO_RS03350 0.35 +0.5 735,142 + HSERO_RS03350 0.38 -0.7 735,318 + HSERO_RS03350 0.50 -1.6
Or see this region's nucleotide sequence