Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03330 and HSERO_RS03335 are separated by 162 nucleotides HSERO_RS03335 and HSERO_RS03340 overlap by 4 nucleotides HSERO_RS03340 and HSERO_RS03345 are separated by 122 nucleotides
HSERO_RS03330: HSERO_RS03330 - branched-chain amino acid aminotransferase, at 730,868 to 731,788
_RS03330
HSERO_RS03335: HSERO_RS03335 - branched-chain amino acid ABC transporter permease, at 731,951 to 732,736
_RS03335
HSERO_RS03340: HSERO_RS03340 - branched-chain amino acid transporter, at 732,733 to 733,056
_RS03340
HSERO_RS03345: HSERO_RS03345 - phosphoglycerate kinase, at 733,179 to 734,369
_RS03345
Position (kb) 731
732
733 Strain fitness (log2 ratio) -2
-1
0
1 at 731.160 kb on + strand, within HSERO_RS03330 at 731.168 kb on - strand, within HSERO_RS03330 at 731.266 kb on - strand, within HSERO_RS03330 at 731.478 kb on + strand, within HSERO_RS03330 at 731.688 kb on + strand, within HSERO_RS03330 at 731.688 kb on - strand, within HSERO_RS03330 at 731.696 kb on - strand, within HSERO_RS03330 at 731.713 kb on - strand at 731.784 kb on - strand at 731.786 kb on - strand at 731.823 kb on - strand at 731.823 kb on - strand at 731.933 kb on - strand at 731.959 kb on + strand at 731.979 kb on + strand at 732.067 kb on - strand, within HSERO_RS03335 at 732.082 kb on + strand, within HSERO_RS03335 at 732.082 kb on + strand, within HSERO_RS03335 at 732.082 kb on + strand, within HSERO_RS03335 at 732.090 kb on - strand, within HSERO_RS03335 at 732.090 kb on - strand, within HSERO_RS03335 at 732.090 kb on - strand, within HSERO_RS03335 at 732.107 kb on + strand, within HSERO_RS03335 at 732.163 kb on - strand, within HSERO_RS03335 at 732.208 kb on + strand, within HSERO_RS03335 at 732.299 kb on + strand, within HSERO_RS03335 at 732.324 kb on + strand, within HSERO_RS03335 at 732.349 kb on + strand, within HSERO_RS03335 at 732.554 kb on + strand, within HSERO_RS03335 at 732.580 kb on + strand, within HSERO_RS03335 at 732.580 kb on + strand, within HSERO_RS03335 at 732.593 kb on - strand, within HSERO_RS03335 at 732.593 kb on - strand, within HSERO_RS03335 at 732.636 kb on - strand, within HSERO_RS03335 at 732.683 kb on - strand at 732.776 kb on + strand, within HSERO_RS03340 at 732.789 kb on - strand, within HSERO_RS03340 at 732.863 kb on - strand, within HSERO_RS03340 at 732.893 kb on + strand, within HSERO_RS03340 at 732.911 kb on - strand, within HSERO_RS03340 at 732.934 kb on - strand, within HSERO_RS03340 at 733.036 kb on + strand at 733.124 kb on + strand at 733.124 kb on + strand at 733.124 kb on + strand at 733.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 731,160 + HSERO_RS03330 0.32 -0.5 731,168 - HSERO_RS03330 0.33 +0.7 731,266 - HSERO_RS03330 0.43 -1.0 731,478 + HSERO_RS03330 0.66 +0.8 731,688 + HSERO_RS03330 0.89 +0.6 731,688 - HSERO_RS03330 0.89 +0.9 731,696 - HSERO_RS03330 0.90 -0.0 731,713 - -0.0 731,784 - +1.6 731,786 - -0.1 731,823 - +0.8 731,823 - +0.2 731,933 - -0.4 731,959 + +0.5 731,979 + +0.0 732,067 - HSERO_RS03335 0.15 +0.8 732,082 + HSERO_RS03335 0.17 -0.5 732,082 + HSERO_RS03335 0.17 +0.1 732,082 + HSERO_RS03335 0.17 -0.3 732,090 - HSERO_RS03335 0.18 -0.3 732,090 - HSERO_RS03335 0.18 +0.7 732,090 - HSERO_RS03335 0.18 +0.7 732,107 + HSERO_RS03335 0.20 -1.7 732,163 - HSERO_RS03335 0.27 -2.2 732,208 + HSERO_RS03335 0.33 -2.0 732,299 + HSERO_RS03335 0.44 -1.8 732,324 + HSERO_RS03335 0.47 -2.6 732,349 + HSERO_RS03335 0.51 -0.3 732,554 + HSERO_RS03335 0.77 -1.5 732,580 + HSERO_RS03335 0.80 -1.1 732,580 + HSERO_RS03335 0.80 -0.2 732,593 - HSERO_RS03335 0.82 -0.1 732,593 - HSERO_RS03335 0.82 +0.2 732,636 - HSERO_RS03335 0.87 +0.3 732,683 - -1.7 732,776 + HSERO_RS03340 0.13 +0.6 732,789 - HSERO_RS03340 0.17 +0.8 732,863 - HSERO_RS03340 0.40 -0.0 732,893 + HSERO_RS03340 0.49 -2.6 732,911 - HSERO_RS03340 0.55 +0.8 732,934 - HSERO_RS03340 0.62 +0.3 733,036 + +0.6 733,124 + -0.4 733,124 + -1.3 733,124 + -0.8 733,132 - -1.2
Or see this region's nucleotide sequence