Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02340 and HSERO_RS02345 are separated by 114 nucleotides HSERO_RS02345 and HSERO_RS02350 overlap by 71 nucleotides HSERO_RS02350 and HSERO_RS02355 are separated by 226 nucleotides
HSERO_RS02340: HSERO_RS02340 - dihydroorotase, at 510,166 to 511,452
_RS02340
HSERO_RS02345: HSERO_RS02345 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 511,567 to 512,367
_RS02345
HSERO_RS02350: HSERO_RS02350 - diadenosine tetraphosphatase, at 512,297 to 513,121
_RS02350
HSERO_RS02355: HSERO_RS02355 - phosphoglucomutase, at 513,348 to 514,724
_RS02355
Position (kb) 511
512
513 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 510.567 kb on + strand, within HSERO_RS02340 at 510.576 kb on - strand, within HSERO_RS02340 at 510.576 kb on - strand, within HSERO_RS02340 at 510.645 kb on - strand, within HSERO_RS02340 at 510.726 kb on - strand, within HSERO_RS02340 at 510.800 kb on + strand, within HSERO_RS02340 at 510.808 kb on - strand, within HSERO_RS02340 at 510.900 kb on + strand, within HSERO_RS02340 at 510.905 kb on + strand, within HSERO_RS02340 at 510.908 kb on + strand, within HSERO_RS02340 at 510.955 kb on - strand, within HSERO_RS02340 at 511.076 kb on + strand, within HSERO_RS02340 at 511.081 kb on + strand, within HSERO_RS02340 at 511.084 kb on - strand, within HSERO_RS02340 at 511.129 kb on + strand, within HSERO_RS02340 at 511.216 kb on - strand, within HSERO_RS02340 at 511.218 kb on + strand, within HSERO_RS02340 at 511.229 kb on + strand, within HSERO_RS02340 at 511.272 kb on - strand, within HSERO_RS02340 at 511.347 kb on - strand at 511.605 kb on + strand at 511.646 kb on + strand at 511.740 kb on - strand, within HSERO_RS02345 at 511.925 kb on - strand, within HSERO_RS02345 at 511.996 kb on + strand, within HSERO_RS02345 at 512.062 kb on + strand, within HSERO_RS02345 at 512.070 kb on - strand, within HSERO_RS02345 at 512.070 kb on - strand, within HSERO_RS02345 at 512.075 kb on - strand, within HSERO_RS02345 at 512.077 kb on - strand, within HSERO_RS02345 at 512.173 kb on + strand, within HSERO_RS02345 at 512.208 kb on + strand, within HSERO_RS02345 at 512.216 kb on - strand, within HSERO_RS02345 at 512.361 kb on + strand at 512.369 kb on - strand at 512.445 kb on + strand, within HSERO_RS02350 at 512.485 kb on + strand, within HSERO_RS02350 at 512.506 kb on - strand, within HSERO_RS02350 at 512.585 kb on + strand, within HSERO_RS02350 at 512.842 kb on - strand, within HSERO_RS02350 at 512.914 kb on + strand, within HSERO_RS02350 at 512.914 kb on + strand, within HSERO_RS02350 at 512.922 kb on - strand, within HSERO_RS02350 at 513.070 kb on + strand at 513.189 kb on + strand at 513.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 510,567 + HSERO_RS02340 0.31 -1.4 510,576 - HSERO_RS02340 0.32 -1.6 510,576 - HSERO_RS02340 0.32 -0.1 510,645 - HSERO_RS02340 0.37 -0.6 510,726 - HSERO_RS02340 0.44 -1.7 510,800 + HSERO_RS02340 0.49 -1.4 510,808 - HSERO_RS02340 0.50 -0.3 510,900 + HSERO_RS02340 0.57 +0.6 510,905 + HSERO_RS02340 0.57 -1.9 510,908 + HSERO_RS02340 0.58 -2.0 510,955 - HSERO_RS02340 0.61 -0.2 511,076 + HSERO_RS02340 0.71 -3.9 511,081 + HSERO_RS02340 0.71 -1.3 511,084 - HSERO_RS02340 0.71 -2.0 511,129 + HSERO_RS02340 0.75 +0.8 511,216 - HSERO_RS02340 0.82 -0.0 511,218 + HSERO_RS02340 0.82 -1.8 511,229 + HSERO_RS02340 0.83 +0.0 511,272 - HSERO_RS02340 0.86 +2.7 511,347 - -1.2 511,605 + -0.6 511,646 + +1.1 511,740 - HSERO_RS02345 0.22 +0.6 511,925 - HSERO_RS02345 0.45 -0.8 511,996 + HSERO_RS02345 0.54 +0.0 512,062 + HSERO_RS02345 0.62 +0.8 512,070 - HSERO_RS02345 0.63 +1.7 512,070 - HSERO_RS02345 0.63 +0.6 512,075 - HSERO_RS02345 0.63 +0.7 512,077 - HSERO_RS02345 0.64 +1.0 512,173 + HSERO_RS02345 0.76 +2.1 512,208 + HSERO_RS02345 0.80 +0.1 512,216 - HSERO_RS02345 0.81 -0.1 512,361 + +0.6 512,369 - +1.7 512,445 + HSERO_RS02350 0.18 +0.0 512,485 + HSERO_RS02350 0.23 -2.4 512,506 - HSERO_RS02350 0.25 +0.3 512,585 + HSERO_RS02350 0.35 -1.7 512,842 - HSERO_RS02350 0.66 +1.0 512,914 + HSERO_RS02350 0.75 +1.3 512,914 + HSERO_RS02350 0.75 +2.1 512,922 - HSERO_RS02350 0.76 -1.3 513,070 + +1.1 513,189 + +0.9 513,364 - +0.7
Or see this region's nucleotide sequence