Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02120 and HSERO_RS02125 are separated by 5 nucleotides HSERO_RS02125 and HSERO_RS02130 are separated by 38 nucleotides HSERO_RS02130 and HSERO_RS02135 are separated by 2 nucleotides HSERO_RS02135 and HSERO_RS02140 are separated by 1 nucleotides
HSERO_RS02120: HSERO_RS02120 - phosphomethylpyrimidine synthase ThiC, at 462,763 to 464,676
_RS02120
HSERO_RS02125: HSERO_RS02125 - thiamine biosynthesis protein ThiS, at 464,682 to 464,882
_RS02125
HSERO_RS02130: HSERO_RS02130 - thiazole synthase, at 464,921 to 465,730
_RS02130
HSERO_RS02135: HSERO_RS02135 - phosphomethylpyrimidine kinase, at 465,733 to 466,599
_RS02135
HSERO_RS02140: HSERO_RS02140 - thiamine-phosphate synthase, at 466,601 to 467,233
_RS02140
Position (kb) 464
465
466 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 463.945 kb on - strand, within HSERO_RS02120 at 464.053 kb on + strand, within HSERO_RS02120 at 464.114 kb on - strand, within HSERO_RS02120 at 464.116 kb on - strand, within HSERO_RS02120 at 464.161 kb on - strand, within HSERO_RS02120 at 464.250 kb on + strand, within HSERO_RS02120 at 464.280 kb on - strand, within HSERO_RS02120 at 464.281 kb on - strand, within HSERO_RS02120 at 464.291 kb on - strand, within HSERO_RS02120 at 464.389 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.417 kb on + strand, within HSERO_RS02120 at 464.503 kb on + strand at 464.508 kb on + strand at 464.532 kb on + strand at 464.552 kb on + strand at 464.560 kb on - strand at 464.622 kb on + strand at 464.627 kb on + strand at 464.739 kb on - strand, within HSERO_RS02125 at 464.741 kb on + strand, within HSERO_RS02125 at 464.749 kb on - strand, within HSERO_RS02125 at 464.752 kb on + strand, within HSERO_RS02125 at 464.834 kb on + strand, within HSERO_RS02125 at 464.834 kb on + strand, within HSERO_RS02125 at 464.849 kb on + strand, within HSERO_RS02125 at 464.959 kb on + strand at 464.960 kb on + strand at 464.968 kb on - strand at 465.016 kb on + strand, within HSERO_RS02130 at 465.163 kb on + strand, within HSERO_RS02130 at 465.255 kb on - strand, within HSERO_RS02130 at 465.286 kb on + strand, within HSERO_RS02130 at 465.311 kb on - strand, within HSERO_RS02130 at 465.393 kb on + strand, within HSERO_RS02130 at 465.448 kb on - strand, within HSERO_RS02130 at 465.584 kb on + strand, within HSERO_RS02130 at 465.821 kb on - strand, within HSERO_RS02135 at 465.869 kb on - strand, within HSERO_RS02135 at 465.885 kb on - strand, within HSERO_RS02135 at 465.895 kb on - strand, within HSERO_RS02135 at 465.925 kb on - strand, within HSERO_RS02135 at 465.953 kb on - strand, within HSERO_RS02135 at 466.006 kb on - strand, within HSERO_RS02135 at 466.197 kb on + strand, within HSERO_RS02135 at 466.263 kb on + strand, within HSERO_RS02135 at 466.474 kb on - strand, within HSERO_RS02135 at 466.485 kb on - strand at 466.487 kb on + strand, within HSERO_RS02135 at 466.567 kb on + strand at 466.682 kb on + strand, within HSERO_RS02140
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 463,945 - HSERO_RS02120 0.62 +0.5 464,053 + HSERO_RS02120 0.67 -0.5 464,114 - HSERO_RS02120 0.71 -0.2 464,116 - HSERO_RS02120 0.71 -0.9 464,161 - HSERO_RS02120 0.73 +0.2 464,250 + HSERO_RS02120 0.78 +0.4 464,280 - HSERO_RS02120 0.79 -0.2 464,281 - HSERO_RS02120 0.79 +0.1 464,291 - HSERO_RS02120 0.80 -1.9 464,389 - HSERO_RS02120 0.85 +0.2 464,399 - HSERO_RS02120 0.85 +1.0 464,399 - HSERO_RS02120 0.85 +1.3 464,399 - HSERO_RS02120 0.85 +0.7 464,417 + HSERO_RS02120 0.86 -3.3 464,503 + +0.7 464,508 + +0.7 464,532 + -0.3 464,552 + +0.1 464,560 - -0.5 464,622 + +0.8 464,627 + +0.3 464,739 - HSERO_RS02125 0.28 -2.6 464,741 + HSERO_RS02125 0.29 +0.1 464,749 - HSERO_RS02125 0.33 -0.4 464,752 + HSERO_RS02125 0.35 +0.6 464,834 + HSERO_RS02125 0.76 -5.0 464,834 + HSERO_RS02125 0.76 -3.1 464,849 + HSERO_RS02125 0.83 +0.3 464,959 + +0.9 464,960 + -0.2 464,968 - -0.3 465,016 + HSERO_RS02130 0.12 -1.0 465,163 + HSERO_RS02130 0.30 +0.7 465,255 - HSERO_RS02130 0.41 +1.0 465,286 + HSERO_RS02130 0.45 +1.4 465,311 - HSERO_RS02130 0.48 -0.1 465,393 + HSERO_RS02130 0.58 +0.5 465,448 - HSERO_RS02130 0.65 -1.0 465,584 + HSERO_RS02130 0.82 +1.7 465,821 - HSERO_RS02135 0.10 +1.6 465,869 - HSERO_RS02135 0.16 +0.9 465,885 - HSERO_RS02135 0.18 +1.8 465,895 - HSERO_RS02135 0.19 -0.1 465,925 - HSERO_RS02135 0.22 +0.8 465,953 - HSERO_RS02135 0.25 +0.3 466,006 - HSERO_RS02135 0.31 +1.2 466,197 + HSERO_RS02135 0.54 +0.9 466,263 + HSERO_RS02135 0.61 +0.6 466,474 - HSERO_RS02135 0.85 +1.0 466,485 - -0.3 466,487 + HSERO_RS02135 0.87 +0.1 466,567 + +0.3 466,682 + HSERO_RS02140 0.13 +0.9
Or see this region's nucleotide sequence