Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02070 and HSERO_RS02075 overlap by 11 nucleotides HSERO_RS02075 and HSERO_RS02080 are separated by 70 nucleotides HSERO_RS02080 and HSERO_RS02085 overlap by 4 nucleotides
HSERO_RS02070: HSERO_RS02070 - deoxyribodipyrimidine photolyase, at 452,261 to 453,463
_RS02070
HSERO_RS02075: HSERO_RS02075 - hypothetical protein, at 453,453 to 453,866
_RS02075
HSERO_RS02080: HSERO_RS02080 - Holliday junction resolvase, at 453,937 to 454,479
_RS02080
HSERO_RS02085: HSERO_RS02085 - membrane protein, at 454,476 to 455,417
_RS02085
Position (kb) 453
454 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 452.608 kb on - strand, within HSERO_RS02070 at 452.774 kb on + strand, within HSERO_RS02070 at 452.869 kb on - strand, within HSERO_RS02070 at 452.973 kb on + strand, within HSERO_RS02070 at 453.009 kb on + strand, within HSERO_RS02070 at 453.251 kb on + strand, within HSERO_RS02070 at 453.328 kb on - strand, within HSERO_RS02070 at 453.366 kb on - strand at 453.443 kb on + strand at 453.606 kb on + strand, within HSERO_RS02075 at 453.702 kb on + strand, within HSERO_RS02075 at 453.755 kb on + strand, within HSERO_RS02075 at 453.814 kb on + strand, within HSERO_RS02075 at 453.875 kb on - strand at 454.001 kb on + strand, within HSERO_RS02080 at 454.373 kb on - strand, within HSERO_RS02080
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 452,608 - HSERO_RS02070 0.29 -1.1 452,774 + HSERO_RS02070 0.43 -0.0 452,869 - HSERO_RS02070 0.51 -1.8 452,973 + HSERO_RS02070 0.59 +1.0 453,009 + HSERO_RS02070 0.62 +0.7 453,251 + HSERO_RS02070 0.82 +2.1 453,328 - HSERO_RS02070 0.89 -0.2 453,366 - +0.9 453,443 + +2.0 453,606 + HSERO_RS02075 0.37 +0.4 453,702 + HSERO_RS02075 0.60 -0.6 453,755 + HSERO_RS02075 0.73 -1.3 453,814 + HSERO_RS02075 0.87 +0.4 453,875 - -4.2 454,001 + HSERO_RS02080 0.12 -2.0 454,373 - HSERO_RS02080 0.80 -1.6
Or see this region's nucleotide sequence