Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02050 and HSERO_RS02055 are separated by 178 nucleotides HSERO_RS02055 and HSERO_RS02060 are separated by 97 nucleotides HSERO_RS02060 and HSERO_RS02065 are separated by 111 nucleotides
HSERO_RS02050: HSERO_RS02050 - tRNA-dihydrouridine synthase B, at 447,600 to 448,622
_RS02050
HSERO_RS02055: HSERO_RS02055 - Fis family transcriptional regulator, at 448,801 to 449,034
_RS02055
HSERO_RS02060: HSERO_RS02060 - purine biosynthesis protein purH, at 449,132 to 450,703
_RS02060
HSERO_RS02065: HSERO_RS02065 - hypothetical protein, at 450,815 to 451,753
_RS02065
Position (kb) 449
450
451 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 448.370 kb on + strand, within HSERO_RS02050 at 448.647 kb on + strand at 448.647 kb on + strand at 448.662 kb on + strand at 448.670 kb on - strand at 448.727 kb on - strand at 448.770 kb on - strand at 448.770 kb on - strand at 448.780 kb on - strand at 449.066 kb on - strand at 450.792 kb on + strand at 450.821 kb on - strand at 450.821 kb on - strand at 451.274 kb on - strand, within HSERO_RS02065 at 451.338 kb on - strand, within HSERO_RS02065 at 451.474 kb on + strand, within HSERO_RS02065 at 451.650 kb on - strand, within HSERO_RS02065
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 448,370 + HSERO_RS02050 0.75 +1.1 448,647 + -1.0 448,647 + +0.5 448,662 + -1.2 448,670 - -3.3 448,727 - -3.0 448,770 - +0.2 448,770 - -0.2 448,780 - -2.3 449,066 - -0.6 450,792 + +0.3 450,821 - -1.4 450,821 - -0.8 451,274 - HSERO_RS02065 0.49 +0.5 451,338 - HSERO_RS02065 0.56 +1.6 451,474 + HSERO_RS02065 0.70 +0.7 451,650 - HSERO_RS02065 0.89 +1.7
Or see this region's nucleotide sequence