Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01985 and HSERO_RS01990 are separated by 88 nucleotides HSERO_RS01990 and HSERO_RS01995 are separated by 26 nucleotides HSERO_RS01995 and HSERO_RS02000 are separated by 220 nucleotides
HSERO_RS01985: HSERO_RS01985 - energy transducer TonB, at 435,269 to 436,150
_RS01985
HSERO_RS01990: HSERO_RS01990 - shikimate 5-dehydrogenase, at 436,239 to 437,054
_RS01990
HSERO_RS01995: HSERO_RS01995 - peptidoglycan transglycosylase, at 437,081 to 437,788
_RS01995
HSERO_RS02000: HSERO_RS02000 - magnesium transporter, at 438,009 to 438,974
_RS02000
Position (kb) 437
438 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 436.226 kb on + strand at 436.449 kb on - strand, within HSERO_RS01990 at 436.484 kb on - strand, within HSERO_RS01990 at 436.551 kb on - strand at 436.570 kb on - strand, within HSERO_RS01990 at 436.853 kb on - strand, within HSERO_RS01990 at 436.875 kb on - strand, within HSERO_RS01990 at 437.112 kb on - strand at 437.117 kb on + strand at 437.117 kb on + strand at 437.132 kb on - strand at 437.167 kb on + strand, within HSERO_RS01995 at 437.167 kb on + strand, within HSERO_RS01995 at 437.175 kb on - strand, within HSERO_RS01995 at 437.651 kb on - strand, within HSERO_RS01995 at 437.817 kb on - strand at 438.073 kb on + strand at 438.100 kb on - strand at 438.105 kb on - strand at 438.425 kb on + strand, within HSERO_RS02000 at 438.540 kb on - strand, within HSERO_RS02000 at 438.548 kb on - strand, within HSERO_RS02000 at 438.646 kb on + strand, within HSERO_RS02000 at 438.646 kb on + strand, within HSERO_RS02000 at 438.656 kb on + strand, within HSERO_RS02000
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 436,226 + -0.8 436,449 - HSERO_RS01990 0.26 -0.7 436,484 - HSERO_RS01990 0.30 +0.0 436,551 - -0.3 436,570 - HSERO_RS01990 0.41 -0.7 436,853 - HSERO_RS01990 0.75 -1.0 436,875 - HSERO_RS01990 0.78 +0.6 437,112 - +0.7 437,117 + +0.4 437,117 + +0.5 437,132 - +1.7 437,167 + HSERO_RS01995 0.12 +0.2 437,167 + HSERO_RS01995 0.12 -0.9 437,175 - HSERO_RS01995 0.13 -2.0 437,651 - HSERO_RS01995 0.81 -0.3 437,817 - -0.6 438,073 + -2.9 438,100 - -0.3 438,105 - +0.3 438,425 + HSERO_RS02000 0.43 -0.8 438,540 - HSERO_RS02000 0.55 -2.0 438,548 - HSERO_RS02000 0.56 -0.6 438,646 + HSERO_RS02000 0.66 -0.7 438,646 + HSERO_RS02000 0.66 -0.9 438,656 + HSERO_RS02000 0.67 +1.1
Or see this region's nucleotide sequence