Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01830 and HSERO_RS01835 are separated by 86 nucleotides HSERO_RS01835 and HSERO_RS01840 are separated by 356 nucleotides HSERO_RS01840 and HSERO_RS01845 overlap by 2 nucleotides HSERO_RS01845 and HSERO_RS01850 are separated by 381 nucleotides
HSERO_RS01830: HSERO_RS01830 - prolyl-tRNA synthetase, at 402,729 to 404,456
_RS01830
HSERO_RS01835: HSERO_RS01835 - hypothetical protein, at 404,543 to 404,956
_RS01835
HSERO_RS01840: HSERO_RS01840 - hypothetical protein, at 405,313 to 405,543
_RS01840
HSERO_RS01845: HSERO_RS01845 - hypothetical protein, at 405,542 to 405,844
_RS01845
HSERO_RS01850: HSERO_RS01850 - signal recognition particle protein, at 406,226 to 407,596
_RS01850
Position (kb) 405
406 Strain fitness (log2 ratio) -2
-1
0
1
2 at 404.746 kb on + strand, within HSERO_RS01835 at 404.882 kb on - strand, within HSERO_RS01835 at 405.068 kb on - strand at 405.106 kb on - strand at 405.228 kb on - strand at 405.316 kb on + strand at 405.324 kb on - strand at 405.713 kb on + strand, within HSERO_RS01845 at 405.735 kb on - strand, within HSERO_RS01845 at 406.089 kb on - strand at 406.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 404,746 + HSERO_RS01835 0.49 -1.5 404,882 - HSERO_RS01835 0.82 -0.1 405,068 - -2.3 405,106 - +0.7 405,228 - +1.8 405,316 + +1.2 405,324 - -1.7 405,713 + HSERO_RS01845 0.56 -0.1 405,735 - HSERO_RS01845 0.64 -1.6 406,089 - +0.2 406,153 + +0.9
Or see this region's nucleotide sequence