Experiment: Sisomicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01495 and HSERO_RS01500 are separated by 116 nucleotides HSERO_RS01500 and HSERO_RS01505 overlap by 4 nucleotides HSERO_RS01505 and HSERO_RS01510 are separated by 55 nucleotides
HSERO_RS01495: HSERO_RS01495 - Fe-S osidoreductase, at 323,802 to 325,328
_RS01495
HSERO_RS01500: HSERO_RS01500 - transketolase, at 325,445 to 326,263
_RS01500
HSERO_RS01505: HSERO_RS01505 - transketolase, at 326,260 to 327,225
_RS01505
HSERO_RS01510: HSERO_RS01510 - membrane protein, at 327,281 to 328,219
_RS01510
Position (kb) 326
327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 325.348 kb on - strand at 325.388 kb on + strand at 325.803 kb on + strand, within HSERO_RS01500 at 325.811 kb on - strand, within HSERO_RS01500 at 325.816 kb on - strand, within HSERO_RS01500 at 325.877 kb on - strand, within HSERO_RS01500 at 326.145 kb on - strand, within HSERO_RS01500 at 326.145 kb on - strand, within HSERO_RS01500 at 326.225 kb on - strand at 326.330 kb on + strand at 326.476 kb on + strand, within HSERO_RS01505 at 326.814 kb on + strand, within HSERO_RS01505 at 326.899 kb on - strand, within HSERO_RS01505 at 326.996 kb on - strand, within HSERO_RS01505 at 327.181 kb on + strand at 327.271 kb on + strand at 327.332 kb on - strand at 327.714 kb on + strand, within HSERO_RS01510 at 327.724 kb on + strand, within HSERO_RS01510 at 327.840 kb on + strand, within HSERO_RS01510 at 327.856 kb on - strand, within HSERO_RS01510 at 327.916 kb on + strand, within HSERO_RS01510 at 327.946 kb on + strand, within HSERO_RS01510 at 328.145 kb on + strand at 328.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.125 mg/ml remove 325,348 - -0.9 325,388 + -0.3 325,803 + HSERO_RS01500 0.44 -1.2 325,811 - HSERO_RS01500 0.45 -0.3 325,816 - HSERO_RS01500 0.45 +1.6 325,877 - HSERO_RS01500 0.53 +0.8 326,145 - HSERO_RS01500 0.85 +0.7 326,145 - HSERO_RS01500 0.85 +1.4 326,225 - -0.6 326,330 + +1.1 326,476 + HSERO_RS01505 0.22 +1.6 326,814 + HSERO_RS01505 0.57 +2.1 326,899 - HSERO_RS01505 0.66 +1.3 326,996 - HSERO_RS01505 0.76 +0.6 327,181 + +1.1 327,271 + -1.7 327,332 - -0.2 327,714 + HSERO_RS01510 0.46 +0.1 327,724 + HSERO_RS01510 0.47 +0.7 327,840 + HSERO_RS01510 0.60 -1.5 327,856 - HSERO_RS01510 0.61 +0.3 327,916 + HSERO_RS01510 0.68 -2.9 327,946 + HSERO_RS01510 0.71 -0.6 328,145 + +0.6 328,145 + +0.2
Or see this region's nucleotide sequence