Experiment: CDM1 without Isoleucine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14565 and BBR_RS14570 overlap by 4 nucleotides BBR_RS14570 and BBR_RS14575 are separated by 14 nucleotides BBR_RS14575 and BBR_RS20835 are separated by 174 nucleotides
BBR_RS14565: BBR_RS14565 - DNA-processing protein DprA, at 1,040,667 to 1,042,286
_RS14565
BBR_RS14570: BBR_RS14570 - ATP-binding protein, at 1,042,283 to 1,043,818
_RS14570
BBR_RS14575: BBR_RS14575 - YraN family protein, at 1,043,833 to 1,044,249
_RS14575
BBR_RS20835: BBR_RS20835 - hypothetical protein, at 1,044,424 to 1,044,884
_RS20835
Position (kb)
1042
1043
1044 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1041.288 kb on + strand, within BBR_RS14565 at 1041.288 kb on + strand, within BBR_RS14565 at 1041.298 kb on + strand, within BBR_RS14565 at 1041.334 kb on + strand, within BBR_RS14565 at 1041.334 kb on + strand, within BBR_RS14565 at 1041.336 kb on + strand, within BBR_RS14565 at 1041.344 kb on + strand, within BBR_RS14565 at 1041.380 kb on + strand, within BBR_RS14565 at 1041.454 kb on + strand, within BBR_RS14565 at 1041.464 kb on + strand, within BBR_RS14565 at 1041.568 kb on + strand, within BBR_RS14565 at 1041.629 kb on - strand, within BBR_RS14565 at 1041.649 kb on + strand, within BBR_RS14565 at 1041.720 kb on + strand, within BBR_RS14565 at 1041.720 kb on + strand, within BBR_RS14565 at 1041.720 kb on + strand, within BBR_RS14565 at 1041.752 kb on + strand, within BBR_RS14565 at 1041.753 kb on + strand, within BBR_RS14565 at 1041.753 kb on + strand, within BBR_RS14565 at 1041.753 kb on + strand, within BBR_RS14565 at 1041.753 kb on + strand, within BBR_RS14565 at 1041.805 kb on + strand, within BBR_RS14565 at 1041.805 kb on + strand, within BBR_RS14565 at 1041.918 kb on + strand, within BBR_RS14565 at 1041.983 kb on - strand, within BBR_RS14565 at 1042.011 kb on - strand, within BBR_RS14565 at 1042.016 kb on - strand, within BBR_RS14565 at 1042.016 kb on - strand, within BBR_RS14565 at 1042.053 kb on - strand, within BBR_RS14565 at 1042.057 kb on + strand, within BBR_RS14565 at 1042.083 kb on - strand, within BBR_RS14565 at 1042.083 kb on - strand, within BBR_RS14565 at 1042.093 kb on - strand, within BBR_RS14565 at 1042.101 kb on - strand, within BBR_RS14565 at 1042.102 kb on - strand, within BBR_RS14565 at 1042.137 kb on + strand at 1042.155 kb on + strand at 1042.155 kb on + strand at 1042.155 kb on + strand at 1042.155 kb on + strand at 1042.155 kb on + strand at 1042.163 kb on - strand at 1042.163 kb on - strand at 1042.163 kb on - strand at 1042.163 kb on - strand at 1042.163 kb on - strand at 1042.235 kb on + strand at 1042.281 kb on + strand at 1042.289 kb on - strand at 1042.311 kb on + strand at 1042.364 kb on + strand at 1042.402 kb on + strand at 1042.436 kb on - strand at 1042.455 kb on - strand, within BBR_RS14570 at 1042.460 kb on - strand, within BBR_RS14570 at 1042.531 kb on - strand, within BBR_RS14570 at 1042.531 kb on - strand, within BBR_RS14570 at 1042.602 kb on + strand, within BBR_RS14570 at 1042.703 kb on + strand, within BBR_RS14570 at 1042.805 kb on + strand, within BBR_RS14570 at 1042.889 kb on - strand, within BBR_RS14570 at 1042.889 kb on - strand, within BBR_RS14570 at 1043.017 kb on - strand, within BBR_RS14570 at 1043.025 kb on + strand, within BBR_RS14570 at 1043.033 kb on - strand, within BBR_RS14570 at 1043.062 kb on + strand, within BBR_RS14570 at 1043.099 kb on + strand, within BBR_RS14570 at 1043.139 kb on + strand, within BBR_RS14570 at 1043.141 kb on - strand, within BBR_RS14570 at 1043.148 kb on + strand, within BBR_RS14570 at 1043.221 kb on - strand, within BBR_RS14570 at 1043.313 kb on + strand, within BBR_RS14570 at 1043.426 kb on + strand, within BBR_RS14570 at 1043.473 kb on - strand, within BBR_RS14570 at 1043.498 kb on + strand, within BBR_RS14570 at 1043.503 kb on + strand, within BBR_RS14570 at 1043.506 kb on - strand, within BBR_RS14570 at 1043.511 kb on - strand, within BBR_RS14570 at 1043.522 kb on + strand, within BBR_RS14570 at 1043.534 kb on + strand, within BBR_RS14570 at 1043.534 kb on + strand, within BBR_RS14570 at 1043.542 kb on - strand, within BBR_RS14570 at 1043.542 kb on - strand, within BBR_RS14570 at 1043.542 kb on - strand, within BBR_RS14570 at 1043.575 kb on + strand, within BBR_RS14570 at 1043.622 kb on + strand, within BBR_RS14570 at 1043.630 kb on + strand, within BBR_RS14570 at 1043.630 kb on + strand, within BBR_RS14570 at 1043.686 kb on + strand at 1043.700 kb on - strand at 1043.708 kb on - strand at 1043.708 kb on - strand at 1043.718 kb on - strand at 1043.734 kb on + strand at 1043.734 kb on + strand at 1043.734 kb on + strand at 1043.734 kb on + strand at 1043.744 kb on + strand at 1043.778 kb on + strand at 1043.783 kb on + strand at 1043.792 kb on + strand at 1043.807 kb on + strand at 1043.815 kb on - strand at 1043.818 kb on + strand at 1043.826 kb on - strand at 1043.826 kb on - strand at 1043.826 kb on - strand at 1043.863 kb on + strand at 1043.863 kb on + strand at 1043.865 kb on + strand at 1043.885 kb on + strand, within BBR_RS14575 at 1043.927 kb on - strand, within BBR_RS14575 at 1044.018 kb on - strand, within BBR_RS14575 at 1044.019 kb on + strand, within BBR_RS14575 at 1044.051 kb on - strand, within BBR_RS14575 at 1044.139 kb on + strand, within BBR_RS14575 at 1044.149 kb on + strand, within BBR_RS14575 at 1044.149 kb on + strand, within BBR_RS14575 at 1044.217 kb on + strand at 1044.257 kb on + strand at 1044.308 kb on - strand at 1044.327 kb on + strand at 1044.327 kb on + strand at 1044.327 kb on + strand at 1044.335 kb on - strand at 1044.370 kb on - strand at 1044.464 kb on + strand at 1044.471 kb on + strand, within BBR_RS20835 at 1044.501 kb on - strand, within BBR_RS20835 at 1044.510 kb on + strand, within BBR_RS20835 at 1044.529 kb on + strand, within BBR_RS20835 at 1044.529 kb on + strand, within BBR_RS20835 at 1044.535 kb on - strand, within BBR_RS20835 at 1044.631 kb on + strand, within BBR_RS20835 at 1044.631 kb on + strand, within BBR_RS20835 at 1044.758 kb on + strand, within BBR_RS20835 at 1044.815 kb on + strand, within BBR_RS20835 at 1044.815 kb on + strand, within BBR_RS20835
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Isoleucine remove 1,041,288 + BBR_RS14565 0.38 -0.5 1,041,288 + BBR_RS14565 0.38 -0.1 1,041,298 + BBR_RS14565 0.39 -0.6 1,041,334 + BBR_RS14565 0.41 -0.1 1,041,334 + BBR_RS14565 0.41 -0.2 1,041,336 + BBR_RS14565 0.41 -1.0 1,041,344 + BBR_RS14565 0.42 -2.2 1,041,380 + BBR_RS14565 0.44 -0.4 1,041,454 + BBR_RS14565 0.49 +0.7 1,041,464 + BBR_RS14565 0.49 +0.1 1,041,568 + BBR_RS14565 0.56 +0.3 1,041,629 - BBR_RS14565 0.59 -0.9 1,041,649 + BBR_RS14565 0.61 +1.0 1,041,720 + BBR_RS14565 0.65 +0.6 1,041,720 + BBR_RS14565 0.65 +0.4 1,041,720 + BBR_RS14565 0.65 +1.2 1,041,752 + BBR_RS14565 0.67 +0.2 1,041,753 + BBR_RS14565 0.67 -0.8 1,041,753 + BBR_RS14565 0.67 -0.9 1,041,753 + BBR_RS14565 0.67 +0.3 1,041,753 + BBR_RS14565 0.67 +0.6 1,041,805 + BBR_RS14565 0.70 +0.4 1,041,805 + BBR_RS14565 0.70 -0.9 1,041,918 + BBR_RS14565 0.77 +0.5 1,041,983 - BBR_RS14565 0.81 -1.5 1,042,011 - BBR_RS14565 0.83 +1.1 1,042,016 - BBR_RS14565 0.83 -1.0 1,042,016 - BBR_RS14565 0.83 +2.7 1,042,053 - BBR_RS14565 0.86 -1.8 1,042,057 + BBR_RS14565 0.86 -0.1 1,042,083 - BBR_RS14565 0.87 -1.8 1,042,083 - BBR_RS14565 0.87 -0.0 1,042,093 - BBR_RS14565 0.88 -1.0 1,042,101 - BBR_RS14565 0.89 +0.5 1,042,102 - BBR_RS14565 0.89 -0.2 1,042,137 + -1.3 1,042,155 + +1.1 1,042,155 + -0.0 1,042,155 + -2.4 1,042,155 + -2.9 1,042,155 + +0.4 1,042,163 - -2.2 1,042,163 - -1.3 1,042,163 - -2.1 1,042,163 - -0.4 1,042,163 - -0.1 1,042,235 + -0.5 1,042,281 + -0.7 1,042,289 - -0.3 1,042,311 + -1.4 1,042,364 + -0.3 1,042,402 + +0.2 1,042,436 - -0.5 1,042,455 - BBR_RS14570 0.11 -0.9 1,042,460 - BBR_RS14570 0.12 -1.7 1,042,531 - BBR_RS14570 0.16 -1.0 1,042,531 - BBR_RS14570 0.16 -0.0 1,042,602 + BBR_RS14570 0.21 -0.5 1,042,703 + BBR_RS14570 0.27 +0.6 1,042,805 + BBR_RS14570 0.34 +1.0 1,042,889 - BBR_RS14570 0.39 -0.9 1,042,889 - BBR_RS14570 0.39 -0.1 1,043,017 - BBR_RS14570 0.48 -0.6 1,043,025 + BBR_RS14570 0.48 -1.1 1,043,033 - BBR_RS14570 0.49 -1.3 1,043,062 + BBR_RS14570 0.51 -0.6 1,043,099 + BBR_RS14570 0.53 -0.7 1,043,139 + BBR_RS14570 0.56 -0.0 1,043,141 - BBR_RS14570 0.56 +1.0 1,043,148 + BBR_RS14570 0.56 -2.8 1,043,221 - BBR_RS14570 0.61 +0.8 1,043,313 + BBR_RS14570 0.67 -2.0 1,043,426 + BBR_RS14570 0.74 -0.1 1,043,473 - BBR_RS14570 0.77 -0.4 1,043,498 + BBR_RS14570 0.79 -0.0 1,043,503 + BBR_RS14570 0.79 -1.0 1,043,506 - BBR_RS14570 0.80 -1.5 1,043,511 - BBR_RS14570 0.80 -1.5 1,043,522 + BBR_RS14570 0.81 -1.4 1,043,534 + BBR_RS14570 0.81 -1.4 1,043,534 + BBR_RS14570 0.81 +0.4 1,043,542 - BBR_RS14570 0.82 -0.4 1,043,542 - BBR_RS14570 0.82 -2.0 1,043,542 - BBR_RS14570 0.82 -1.7 1,043,575 + BBR_RS14570 0.84 -0.3 1,043,622 + BBR_RS14570 0.87 -0.4 1,043,630 + BBR_RS14570 0.88 +1.7 1,043,630 + BBR_RS14570 0.88 -0.7 1,043,686 + -1.5 1,043,700 - +1.0 1,043,708 - +0.7 1,043,708 - -0.9 1,043,718 - -0.0 1,043,734 + -1.7 1,043,734 + -0.4 1,043,734 + -0.1 1,043,734 + -0.7 1,043,744 + -1.3 1,043,778 + +1.0 1,043,783 + -1.1 1,043,792 + -1.9 1,043,807 + -0.6 1,043,815 - -1.4 1,043,818 + -1.0 1,043,826 - +0.3 1,043,826 - -2.2 1,043,826 - +0.1 1,043,863 + -1.1 1,043,863 + -1.8 1,043,865 + -1.8 1,043,885 + BBR_RS14575 0.12 +0.1 1,043,927 - BBR_RS14575 0.23 -1.2 1,044,018 - BBR_RS14575 0.44 -2.0 1,044,019 + BBR_RS14575 0.45 -1.2 1,044,051 - BBR_RS14575 0.52 -1.3 1,044,139 + BBR_RS14575 0.73 -1.0 1,044,149 + BBR_RS14575 0.76 -3.1 1,044,149 + BBR_RS14575 0.76 -0.4 1,044,217 + -0.0 1,044,257 + -0.1 1,044,308 - -0.1 1,044,327 + -0.3 1,044,327 + +0.8 1,044,327 + +0.3 1,044,335 - +0.8 1,044,370 - -2.7 1,044,464 + -3.6 1,044,471 + BBR_RS20835 0.10 +0.4 1,044,501 - BBR_RS20835 0.17 -2.9 1,044,510 + BBR_RS20835 0.19 +1.0 1,044,529 + BBR_RS20835 0.23 -0.9 1,044,529 + BBR_RS20835 0.23 +1.4 1,044,535 - BBR_RS20835 0.24 -0.4 1,044,631 + BBR_RS20835 0.45 +0.3 1,044,631 + BBR_RS20835 0.45 +0.9 1,044,758 + BBR_RS20835 0.72 -1.9 1,044,815 + BBR_RS20835 0.85 -0.2 1,044,815 + BBR_RS20835 0.85 -0.2
Or see this region's nucleotide sequence