Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08645 and MMJJ_RS08650 are separated by 50 nucleotides MMJJ_RS08650 and MMJJ_RS08655 overlap by 4 nucleotides MMJJ_RS08655 and MMJJ_RS08660 are separated by 156 nucleotides
MMJJ_RS08645: MMJJ_RS08645 - aspartate carbamoyltransferase regulatory subunit, at 1,591,484 to 1,591,930
_RS08645
MMJJ_RS08650: MMJJ_RS08650 - TfuA-like protein, at 1,591,981 to 1,592,625
_RS08650
MMJJ_RS08655: MMJJ_RS08655 - phospholipase D-like domain-containing protein, at 1,592,622 to 1,593,197
_RS08655
MMJJ_RS08660: MMJJ_RS08660 - acetylornithine transaminase, at 1,593,354 to 1,594,541
_RS08660
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1591.683 kb on + strand, within MMJJ_RS08645 at 1591.953 kb on - strand at 1591.997 kb on - strand at 1591.997 kb on - strand at 1591.997 kb on - strand at 1591.997 kb on - strand at 1591.998 kb on + strand at 1591.998 kb on + strand at 1591.998 kb on + strand at 1591.998 kb on + strand at 1592.003 kb on + strand at 1592.041 kb on - strand at 1592.041 kb on - strand at 1592.041 kb on - strand at 1592.043 kb on - strand at 1592.043 kb on - strand at 1592.044 kb on + strand at 1592.130 kb on - strand, within MMJJ_RS08650 at 1592.131 kb on + strand, within MMJJ_RS08650 at 1592.131 kb on + strand, within MMJJ_RS08650 at 1592.131 kb on + strand, within MMJJ_RS08650 at 1592.141 kb on - strand, within MMJJ_RS08650 at 1592.141 kb on - strand, within MMJJ_RS08650 at 1592.142 kb on + strand, within MMJJ_RS08650 at 1592.142 kb on + strand, within MMJJ_RS08650 at 1592.142 kb on + strand, within MMJJ_RS08650 at 1592.143 kb on - strand, within MMJJ_RS08650 at 1592.143 kb on - strand, within MMJJ_RS08650 at 1592.143 kb on - strand, within MMJJ_RS08650 at 1592.144 kb on + strand, within MMJJ_RS08650 at 1592.161 kb on + strand, within MMJJ_RS08650 at 1592.211 kb on - strand, within MMJJ_RS08650 at 1592.257 kb on - strand, within MMJJ_RS08650 at 1592.263 kb on + strand, within MMJJ_RS08650 at 1592.263 kb on + strand, within MMJJ_RS08650 at 1592.274 kb on - strand, within MMJJ_RS08650 at 1592.275 kb on + strand, within MMJJ_RS08650 at 1592.275 kb on + strand, within MMJJ_RS08650 at 1592.302 kb on - strand, within MMJJ_RS08650 at 1592.303 kb on + strand, within MMJJ_RS08650 at 1592.317 kb on - strand, within MMJJ_RS08650 at 1592.322 kb on - strand, within MMJJ_RS08650 at 1592.356 kb on + strand, within MMJJ_RS08650 at 1592.375 kb on + strand, within MMJJ_RS08650 at 1592.401 kb on - strand, within MMJJ_RS08650 at 1592.401 kb on - strand, within MMJJ_RS08650 at 1592.406 kb on - strand, within MMJJ_RS08650 at 1592.407 kb on + strand, within MMJJ_RS08650 at 1592.407 kb on + strand, within MMJJ_RS08650 at 1592.407 kb on + strand, within MMJJ_RS08650 at 1592.409 kb on - strand, within MMJJ_RS08650 at 1592.410 kb on + strand, within MMJJ_RS08650 at 1592.412 kb on - strand, within MMJJ_RS08650 at 1592.454 kb on - strand, within MMJJ_RS08650 at 1592.454 kb on - strand, within MMJJ_RS08650 at 1592.455 kb on + strand, within MMJJ_RS08650 at 1592.455 kb on + strand, within MMJJ_RS08650 at 1592.491 kb on + strand, within MMJJ_RS08650 at 1592.491 kb on + strand, within MMJJ_RS08650 at 1592.495 kb on - strand, within MMJJ_RS08650 at 1592.496 kb on + strand, within MMJJ_RS08650 at 1592.545 kb on - strand, within MMJJ_RS08650 at 1592.545 kb on - strand, within MMJJ_RS08650 at 1592.546 kb on + strand, within MMJJ_RS08650 at 1592.546 kb on + strand, within MMJJ_RS08650 at 1592.579 kb on + strand at 1592.595 kb on - strand at 1592.595 kb on - strand at 1592.613 kb on - strand at 1592.685 kb on + strand, within MMJJ_RS08655 at 1592.685 kb on + strand, within MMJJ_RS08655 at 1592.685 kb on + strand, within MMJJ_RS08655 at 1592.708 kb on - strand, within MMJJ_RS08655 at 1592.759 kb on - strand, within MMJJ_RS08655 at 1592.760 kb on + strand, within MMJJ_RS08655 at 1592.770 kb on + strand, within MMJJ_RS08655 at 1592.770 kb on + strand, within MMJJ_RS08655 at 1592.775 kb on - strand, within MMJJ_RS08655 at 1592.780 kb on - strand, within MMJJ_RS08655 at 1592.781 kb on + strand, within MMJJ_RS08655 at 1592.781 kb on + strand, within MMJJ_RS08655 at 1592.783 kb on - strand, within MMJJ_RS08655 at 1592.784 kb on + strand, within MMJJ_RS08655 at 1592.796 kb on + strand, within MMJJ_RS08655 at 1592.820 kb on + strand, within MMJJ_RS08655 at 1592.852 kb on - strand, within MMJJ_RS08655 at 1592.880 kb on - strand, within MMJJ_RS08655 at 1592.928 kb on - strand, within MMJJ_RS08655 at 1592.929 kb on + strand, within MMJJ_RS08655 at 1592.929 kb on + strand, within MMJJ_RS08655 at 1592.949 kb on - strand, within MMJJ_RS08655 at 1592.949 kb on - strand, within MMJJ_RS08655 at 1592.949 kb on - strand, within MMJJ_RS08655 at 1592.949 kb on - strand, within MMJJ_RS08655 at 1592.950 kb on + strand, within MMJJ_RS08655 at 1592.950 kb on + strand, within MMJJ_RS08655 at 1592.954 kb on + strand, within MMJJ_RS08655 at 1592.970 kb on + strand, within MMJJ_RS08655 at 1592.975 kb on - strand, within MMJJ_RS08655 at 1592.988 kb on + strand, within MMJJ_RS08655 at 1592.992 kb on - strand, within MMJJ_RS08655 at 1592.992 kb on - strand, within MMJJ_RS08655 at 1592.992 kb on - strand, within MMJJ_RS08655 at 1592.993 kb on + strand, within MMJJ_RS08655 at 1593.007 kb on + strand, within MMJJ_RS08655 at 1593.007 kb on + strand, within MMJJ_RS08655 at 1593.013 kb on - strand, within MMJJ_RS08655 at 1593.013 kb on - strand, within MMJJ_RS08655 at 1593.013 kb on - strand, within MMJJ_RS08655 at 1593.014 kb on + strand, within MMJJ_RS08655 at 1593.014 kb on + strand, within MMJJ_RS08655 at 1593.014 kb on + strand, within MMJJ_RS08655 at 1593.022 kb on - strand, within MMJJ_RS08655 at 1593.022 kb on - strand, within MMJJ_RS08655 at 1593.089 kb on - strand, within MMJJ_RS08655 at 1593.115 kb on + strand, within MMJJ_RS08655 at 1593.129 kb on - strand, within MMJJ_RS08655 at 1593.153 kb on - strand at 1593.155 kb on - strand at 1593.155 kb on - strand at 1593.174 kb on - strand at 1593.174 kb on - strand at 1593.174 kb on - strand at 1593.175 kb on + strand at 1593.176 kb on - strand at 1593.176 kb on - strand at 1593.201 kb on + strand at 1593.201 kb on + strand at 1593.201 kb on + strand at 1593.210 kb on + strand at 1593.210 kb on + strand at 1593.221 kb on - strand at 1593.221 kb on - strand at 1593.252 kb on - strand at 1593.269 kb on - strand at 1593.269 kb on - strand at 1593.270 kb on + strand at 1593.270 kb on + strand at 1593.290 kb on - strand at 1593.291 kb on + strand at 1593.291 kb on + strand at 1593.291 kb on + strand at 1593.313 kb on + strand at 1593.317 kb on + strand at 1593.594 kb on - strand, within MMJJ_RS08660 at 1593.619 kb on - strand at 1593.785 kb on - strand, within MMJJ_RS08660 at 1593.970 kb on + strand, within MMJJ_RS08660 at 1593.991 kb on - strand, within MMJJ_RS08660 at 1593.992 kb on + strand, within MMJJ_RS08660 at 1594.061 kb on + strand, within MMJJ_RS08660 at 1594.099 kb on - strand, within MMJJ_RS08660
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,591,683 + MMJJ_RS08645 0.45 -1.2 1,591,953 - -3.0 1,591,997 - +1.0 1,591,997 - -0.1 1,591,997 - +0.5 1,591,997 - -1.2 1,591,998 + -0.6 1,591,998 + -1.2 1,591,998 + +1.4 1,591,998 + -0.3 1,592,003 + -0.6 1,592,041 - -0.8 1,592,041 - -1.9 1,592,041 - -2.3 1,592,043 - -0.6 1,592,043 - -0.8 1,592,044 + -0.8 1,592,130 - MMJJ_RS08650 0.23 -0.6 1,592,131 + MMJJ_RS08650 0.23 -2.8 1,592,131 + MMJJ_RS08650 0.23 -1.8 1,592,131 + MMJJ_RS08650 0.23 -1.9 1,592,141 - MMJJ_RS08650 0.25 -0.0 1,592,141 - MMJJ_RS08650 0.25 -3.8 1,592,142 + MMJJ_RS08650 0.25 -1.0 1,592,142 + MMJJ_RS08650 0.25 -1.9 1,592,142 + MMJJ_RS08650 0.25 -1.6 1,592,143 - MMJJ_RS08650 0.25 -1.7 1,592,143 - MMJJ_RS08650 0.25 -1.1 1,592,143 - MMJJ_RS08650 0.25 -2.6 1,592,144 + MMJJ_RS08650 0.25 -1.1 1,592,161 + MMJJ_RS08650 0.28 -4.2 1,592,211 - MMJJ_RS08650 0.36 -1.6 1,592,257 - MMJJ_RS08650 0.43 -1.2 1,592,263 + MMJJ_RS08650 0.44 -0.3 1,592,263 + MMJJ_RS08650 0.44 -2.6 1,592,274 - MMJJ_RS08650 0.45 -1.0 1,592,275 + MMJJ_RS08650 0.46 -1.2 1,592,275 + MMJJ_RS08650 0.46 -2.6 1,592,302 - MMJJ_RS08650 0.50 -1.1 1,592,303 + MMJJ_RS08650 0.50 -1.5 1,592,317 - MMJJ_RS08650 0.52 -1.5 1,592,322 - MMJJ_RS08650 0.53 -1.6 1,592,356 + MMJJ_RS08650 0.58 -1.3 1,592,375 + MMJJ_RS08650 0.61 -1.3 1,592,401 - MMJJ_RS08650 0.65 -1.1 1,592,401 - MMJJ_RS08650 0.65 -0.9 1,592,406 - MMJJ_RS08650 0.66 -1.2 1,592,407 + MMJJ_RS08650 0.66 -0.2 1,592,407 + MMJJ_RS08650 0.66 -1.6 1,592,407 + MMJJ_RS08650 0.66 -0.8 1,592,409 - MMJJ_RS08650 0.66 -0.7 1,592,410 + MMJJ_RS08650 0.67 -1.7 1,592,412 - MMJJ_RS08650 0.67 -1.2 1,592,454 - MMJJ_RS08650 0.73 -0.7 1,592,454 - MMJJ_RS08650 0.73 -1.3 1,592,455 + MMJJ_RS08650 0.73 -2.8 1,592,455 + MMJJ_RS08650 0.73 -0.6 1,592,491 + MMJJ_RS08650 0.79 -1.8 1,592,491 + MMJJ_RS08650 0.79 -1.2 1,592,495 - MMJJ_RS08650 0.80 -0.5 1,592,496 + MMJJ_RS08650 0.80 -0.2 1,592,545 - MMJJ_RS08650 0.87 -1.7 1,592,545 - MMJJ_RS08650 0.87 -2.9 1,592,546 + MMJJ_RS08650 0.88 -0.5 1,592,546 + MMJJ_RS08650 0.88 -1.7 1,592,579 + -1.3 1,592,595 - -1.5 1,592,595 - -0.7 1,592,613 - -1.9 1,592,685 + MMJJ_RS08655 0.11 -4.1 1,592,685 + MMJJ_RS08655 0.11 -4.3 1,592,685 + MMJJ_RS08655 0.11 -3.7 1,592,708 - MMJJ_RS08655 0.15 -4.3 1,592,759 - MMJJ_RS08655 0.24 -2.3 1,592,760 + MMJJ_RS08655 0.24 -3.4 1,592,770 + MMJJ_RS08655 0.26 -3.3 1,592,770 + MMJJ_RS08655 0.26 -4.0 1,592,775 - MMJJ_RS08655 0.27 -5.0 1,592,780 - MMJJ_RS08655 0.27 -4.9 1,592,781 + MMJJ_RS08655 0.28 -6.0 1,592,781 + MMJJ_RS08655 0.28 -3.8 1,592,783 - MMJJ_RS08655 0.28 -4.7 1,592,784 + MMJJ_RS08655 0.28 -4.2 1,592,796 + MMJJ_RS08655 0.30 -3.6 1,592,820 + MMJJ_RS08655 0.34 -4.3 1,592,852 - MMJJ_RS08655 0.40 -0.8 1,592,880 - MMJJ_RS08655 0.45 -3.8 1,592,928 - MMJJ_RS08655 0.53 -4.5 1,592,929 + MMJJ_RS08655 0.53 -2.8 1,592,929 + MMJJ_RS08655 0.53 -4.3 1,592,949 - MMJJ_RS08655 0.57 -5.4 1,592,949 - MMJJ_RS08655 0.57 -4.0 1,592,949 - MMJJ_RS08655 0.57 -4.4 1,592,949 - MMJJ_RS08655 0.57 -2.9 1,592,950 + MMJJ_RS08655 0.57 -4.3 1,592,950 + MMJJ_RS08655 0.57 -1.8 1,592,954 + MMJJ_RS08655 0.58 -4.2 1,592,970 + MMJJ_RS08655 0.60 -3.6 1,592,975 - MMJJ_RS08655 0.61 -0.7 1,592,988 + MMJJ_RS08655 0.64 -4.3 1,592,992 - MMJJ_RS08655 0.64 -5.1 1,592,992 - MMJJ_RS08655 0.64 -4.2 1,592,992 - MMJJ_RS08655 0.64 -4.8 1,592,993 + MMJJ_RS08655 0.64 -4.6 1,593,007 + MMJJ_RS08655 0.67 -3.7 1,593,007 + MMJJ_RS08655 0.67 -3.8 1,593,013 - MMJJ_RS08655 0.68 -1.2 1,593,013 - MMJJ_RS08655 0.68 -2.3 1,593,013 - MMJJ_RS08655 0.68 -2.5 1,593,014 + MMJJ_RS08655 0.68 -3.3 1,593,014 + MMJJ_RS08655 0.68 -3.4 1,593,014 + MMJJ_RS08655 0.68 -2.2 1,593,022 - MMJJ_RS08655 0.69 -4.8 1,593,022 - MMJJ_RS08655 0.69 -4.2 1,593,089 - MMJJ_RS08655 0.81 -4.6 1,593,115 + MMJJ_RS08655 0.86 -3.5 1,593,129 - MMJJ_RS08655 0.88 -4.5 1,593,153 - -0.6 1,593,155 - -3.8 1,593,155 - -4.3 1,593,174 - -2.7 1,593,174 - -0.9 1,593,174 - -2.1 1,593,175 + -5.1 1,593,176 - -2.7 1,593,176 - -1.6 1,593,201 + -2.1 1,593,201 + -3.3 1,593,201 + +0.6 1,593,210 + -4.4 1,593,210 + -3.1 1,593,221 - -0.5 1,593,221 - -2.8 1,593,252 - -2.5 1,593,269 - -1.6 1,593,269 - +0.0 1,593,270 + -0.6 1,593,270 + -4.3 1,593,290 - -2.9 1,593,291 + -3.0 1,593,291 + -1.9 1,593,291 + -0.7 1,593,313 + -1.4 1,593,317 + -1.2 1,593,594 - MMJJ_RS08660 0.20 -2.5 1,593,619 - -1.2 1,593,785 - MMJJ_RS08660 0.36 -2.2 1,593,970 + MMJJ_RS08660 0.52 -2.3 1,593,991 - MMJJ_RS08660 0.54 -1.2 1,593,992 + MMJJ_RS08660 0.54 -0.6 1,594,061 + MMJJ_RS08660 0.60 -1.6 1,594,099 - MMJJ_RS08660 0.63 -1.4
Or see this region's nucleotide sequence