Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08360 and MMJJ_RS08365 are separated by 37 nucleotides MMJJ_RS08365 and MMJJ_RS08370 are separated by 35 nucleotides MMJJ_RS08370 and MMJJ_RS08375 are separated by 18 nucleotides MMJJ_RS08375 and MMJJ_RS08380 are separated by 22 nucleotides
MMJJ_RS08360: MMJJ_RS08360 - FprA family A-type flavoprotein, at 1,541,583 to 1,542,746
_RS08360
MMJJ_RS08365: MMJJ_RS08365 - DsrE family protein, at 1,542,784 to 1,543,134
_RS08365
MMJJ_RS08370: MMJJ_RS08370 - hypothetical protein, at 1,543,170 to 1,543,358
_RS08370
MMJJ_RS08375: MMJJ_RS08375 - ferritin, at 1,543,377 to 1,543,892
_RS08375
MMJJ_RS08380: MMJJ_RS08380 - hypothetical protein, at 1,543,915 to 1,544,181
_RS08380
Position (kb) 1542
1543
1544 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1541.819 kb on + strand, within MMJJ_RS08360 at 1541.825 kb on - strand, within MMJJ_RS08360 at 1541.826 kb on + strand, within MMJJ_RS08360 at 1541.832 kb on + strand, within MMJJ_RS08360 at 1541.857 kb on - strand, within MMJJ_RS08360 at 1541.858 kb on + strand, within MMJJ_RS08360 at 1541.883 kb on - strand, within MMJJ_RS08360 at 1541.888 kb on - strand, within MMJJ_RS08360 at 1541.889 kb on + strand, within MMJJ_RS08360 at 1541.892 kb on - strand, within MMJJ_RS08360 at 1541.893 kb on + strand, within MMJJ_RS08360 at 1541.903 kb on - strand, within MMJJ_RS08360 at 1541.903 kb on - strand, within MMJJ_RS08360 at 1541.957 kb on - strand, within MMJJ_RS08360 at 1541.986 kb on - strand, within MMJJ_RS08360 at 1541.987 kb on + strand, within MMJJ_RS08360 at 1541.987 kb on + strand, within MMJJ_RS08360 at 1541.999 kb on + strand, within MMJJ_RS08360 at 1542.001 kb on + strand, within MMJJ_RS08360 at 1542.018 kb on + strand, within MMJJ_RS08360 at 1542.047 kb on + strand, within MMJJ_RS08360 at 1542.102 kb on - strand, within MMJJ_RS08360 at 1542.103 kb on + strand, within MMJJ_RS08360 at 1542.103 kb on + strand, within MMJJ_RS08360 at 1542.149 kb on - strand, within MMJJ_RS08360 at 1542.171 kb on - strand, within MMJJ_RS08360 at 1542.172 kb on + strand, within MMJJ_RS08360 at 1542.243 kb on - strand, within MMJJ_RS08360 at 1542.277 kb on + strand, within MMJJ_RS08360 at 1542.282 kb on + strand, within MMJJ_RS08360 at 1542.296 kb on + strand, within MMJJ_RS08360 at 1542.363 kb on + strand, within MMJJ_RS08360 at 1542.390 kb on - strand, within MMJJ_RS08360 at 1542.391 kb on + strand, within MMJJ_RS08360 at 1542.392 kb on - strand, within MMJJ_RS08360 at 1542.392 kb on - strand, within MMJJ_RS08360 at 1542.392 kb on - strand, within MMJJ_RS08360 at 1542.393 kb on + strand, within MMJJ_RS08360 at 1542.394 kb on - strand, within MMJJ_RS08360 at 1542.394 kb on - strand, within MMJJ_RS08360 at 1542.420 kb on - strand, within MMJJ_RS08360 at 1542.421 kb on + strand, within MMJJ_RS08360 at 1542.421 kb on + strand, within MMJJ_RS08360 at 1542.522 kb on - strand, within MMJJ_RS08360 at 1542.532 kb on - strand, within MMJJ_RS08360 at 1542.533 kb on + strand, within MMJJ_RS08360 at 1542.533 kb on + strand, within MMJJ_RS08360 at 1542.533 kb on + strand, within MMJJ_RS08360 at 1542.605 kb on - strand, within MMJJ_RS08360 at 1542.606 kb on + strand, within MMJJ_RS08360 at 1542.606 kb on + strand, within MMJJ_RS08360 at 1542.610 kb on + strand, within MMJJ_RS08360 at 1542.612 kb on - strand, within MMJJ_RS08360 at 1542.612 kb on - strand, within MMJJ_RS08360 at 1542.612 kb on - strand, within MMJJ_RS08360 at 1542.635 kb on - strand at 1542.636 kb on + strand at 1542.640 kb on - strand at 1542.674 kb on + strand at 1542.718 kb on - strand at 1542.733 kb on + strand at 1542.737 kb on - strand at 1542.751 kb on - strand at 1542.758 kb on + strand at 1542.780 kb on - strand at 1542.781 kb on + strand at 1542.781 kb on + strand at 1542.781 kb on + strand at 1542.785 kb on - strand at 1542.877 kb on + strand, within MMJJ_RS08365 at 1542.877 kb on + strand, within MMJJ_RS08365 at 1542.877 kb on + strand, within MMJJ_RS08365 at 1542.879 kb on - strand, within MMJJ_RS08365 at 1542.879 kb on - strand, within MMJJ_RS08365 at 1542.879 kb on - strand, within MMJJ_RS08365 at 1542.880 kb on + strand, within MMJJ_RS08365 at 1542.880 kb on + strand, within MMJJ_RS08365 at 1542.884 kb on + strand, within MMJJ_RS08365 at 1542.897 kb on - strand at 1542.900 kb on - strand, within MMJJ_RS08365 at 1543.022 kb on + strand, within MMJJ_RS08365 at 1543.073 kb on + strand, within MMJJ_RS08365 at 1543.085 kb on - strand, within MMJJ_RS08365 at 1543.102 kb on + strand at 1543.107 kb on + strand at 1543.110 kb on - strand at 1543.110 kb on - strand at 1543.110 kb on - strand at 1543.111 kb on + strand at 1543.111 kb on + strand at 1543.111 kb on + strand at 1543.159 kb on - strand at 1543.160 kb on + strand at 1543.171 kb on - strand at 1543.171 kb on - strand at 1543.173 kb on - strand at 1543.241 kb on - strand, within MMJJ_RS08370 at 1543.241 kb on - strand, within MMJJ_RS08370 at 1543.252 kb on + strand, within MMJJ_RS08370 at 1543.252 kb on + strand, within MMJJ_RS08370 at 1543.323 kb on - strand, within MMJJ_RS08370 at 1543.323 kb on - strand, within MMJJ_RS08370 at 1543.324 kb on + strand, within MMJJ_RS08370 at 1543.324 kb on + strand, within MMJJ_RS08370 at 1543.324 kb on + strand, within MMJJ_RS08370 at 1543.327 kb on - strand, within MMJJ_RS08370 at 1543.353 kb on - strand at 1543.353 kb on - strand at 1543.358 kb on - strand at 1543.358 kb on - strand at 1543.359 kb on + strand at 1543.359 kb on + strand at 1543.359 kb on + strand at 1543.378 kb on - strand at 1543.379 kb on + strand at 1543.385 kb on - strand at 1543.447 kb on - strand, within MMJJ_RS08375 at 1543.448 kb on + strand, within MMJJ_RS08375 at 1543.452 kb on - strand, within MMJJ_RS08375 at 1543.452 kb on - strand, within MMJJ_RS08375 at 1543.453 kb on + strand, within MMJJ_RS08375 at 1543.453 kb on + strand, within MMJJ_RS08375 at 1543.453 kb on + strand, within MMJJ_RS08375 at 1543.453 kb on + strand, within MMJJ_RS08375 at 1543.453 kb on + strand, within MMJJ_RS08375 at 1543.467 kb on - strand, within MMJJ_RS08375 at 1543.524 kb on - strand, within MMJJ_RS08375 at 1543.556 kb on + strand, within MMJJ_RS08375 at 1543.564 kb on + strand, within MMJJ_RS08375 at 1543.609 kb on - strand, within MMJJ_RS08375 at 1543.610 kb on + strand, within MMJJ_RS08375 at 1543.610 kb on + strand, within MMJJ_RS08375 at 1543.623 kb on + strand, within MMJJ_RS08375 at 1543.644 kb on - strand, within MMJJ_RS08375 at 1543.644 kb on - strand, within MMJJ_RS08375 at 1543.644 kb on - strand, within MMJJ_RS08375 at 1543.645 kb on + strand, within MMJJ_RS08375 at 1543.645 kb on + strand, within MMJJ_RS08375 at 1543.646 kb on - strand, within MMJJ_RS08375 at 1543.647 kb on + strand, within MMJJ_RS08375 at 1543.650 kb on + strand, within MMJJ_RS08375 at 1543.713 kb on - strand, within MMJJ_RS08375 at 1543.713 kb on - strand, within MMJJ_RS08375 at 1543.713 kb on - strand, within MMJJ_RS08375 at 1543.714 kb on + strand, within MMJJ_RS08375 at 1543.714 kb on + strand, within MMJJ_RS08375 at 1543.714 kb on + strand, within MMJJ_RS08375 at 1543.717 kb on - strand, within MMJJ_RS08375 at 1543.717 kb on - strand, within MMJJ_RS08375 at 1543.718 kb on + strand, within MMJJ_RS08375 at 1543.807 kb on - strand, within MMJJ_RS08375 at 1543.808 kb on + strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.828 kb on + strand, within MMJJ_RS08375 at 1543.828 kb on + strand, within MMJJ_RS08375 at 1543.828 kb on + strand, within MMJJ_RS08375 at 1543.831 kb on - strand, within MMJJ_RS08375 at 1543.831 kb on - strand, within MMJJ_RS08375 at 1543.831 kb on - strand, within MMJJ_RS08375 at 1543.831 kb on - strand, within MMJJ_RS08375 at 1543.832 kb on + strand, within MMJJ_RS08375 at 1543.840 kb on - strand, within MMJJ_RS08375 at 1543.841 kb on + strand at 1543.866 kb on - strand at 1543.867 kb on + strand at 1543.878 kb on - strand at 1543.879 kb on + strand at 1543.879 kb on + strand at 1543.879 kb on + strand at 1543.879 kb on + strand at 1543.883 kb on - strand at 1543.892 kb on - strand at 1543.909 kb on - strand at 1543.909 kb on - strand at 1543.910 kb on + strand at 1543.920 kb on - strand at 1543.921 kb on + strand at 1543.921 kb on + strand at 1543.936 kb on + strand at 1543.990 kb on - strand, within MMJJ_RS08380 at 1544.018 kb on + strand at 1544.018 kb on + strand, within MMJJ_RS08380 at 1544.018 kb on + strand, within MMJJ_RS08380 at 1544.090 kb on + strand, within MMJJ_RS08380
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,541,819 + MMJJ_RS08360 0.20 +0.3 1,541,825 - MMJJ_RS08360 0.21 -0.0 1,541,826 + MMJJ_RS08360 0.21 +1.9 1,541,832 + MMJJ_RS08360 0.21 +0.8 1,541,857 - MMJJ_RS08360 0.24 +0.8 1,541,858 + MMJJ_RS08360 0.24 +2.1 1,541,883 - MMJJ_RS08360 0.26 -1.5 1,541,888 - MMJJ_RS08360 0.26 -0.8 1,541,889 + MMJJ_RS08360 0.26 -0.7 1,541,892 - MMJJ_RS08360 0.27 -1.9 1,541,893 + MMJJ_RS08360 0.27 +1.7 1,541,903 - MMJJ_RS08360 0.27 +1.2 1,541,903 - MMJJ_RS08360 0.27 -0.4 1,541,957 - MMJJ_RS08360 0.32 +1.2 1,541,986 - MMJJ_RS08360 0.35 +0.8 1,541,987 + MMJJ_RS08360 0.35 +0.3 1,541,987 + MMJJ_RS08360 0.35 -0.6 1,541,999 + MMJJ_RS08360 0.36 +1.0 1,542,001 + MMJJ_RS08360 0.36 -0.0 1,542,018 + MMJJ_RS08360 0.37 +0.1 1,542,047 + MMJJ_RS08360 0.40 +0.5 1,542,102 - MMJJ_RS08360 0.45 +0.6 1,542,103 + MMJJ_RS08360 0.45 +0.4 1,542,103 + MMJJ_RS08360 0.45 +0.1 1,542,149 - MMJJ_RS08360 0.49 +1.4 1,542,171 - MMJJ_RS08360 0.51 +0.7 1,542,172 + MMJJ_RS08360 0.51 -0.1 1,542,243 - MMJJ_RS08360 0.57 +0.2 1,542,277 + MMJJ_RS08360 0.60 +1.5 1,542,282 + MMJJ_RS08360 0.60 +0.1 1,542,296 + MMJJ_RS08360 0.61 -0.2 1,542,363 + MMJJ_RS08360 0.67 +2.0 1,542,390 - MMJJ_RS08360 0.69 +0.2 1,542,391 + MMJJ_RS08360 0.69 -0.5 1,542,392 - MMJJ_RS08360 0.70 +1.3 1,542,392 - MMJJ_RS08360 0.70 +0.3 1,542,392 - MMJJ_RS08360 0.70 +0.7 1,542,393 + MMJJ_RS08360 0.70 +0.8 1,542,394 - MMJJ_RS08360 0.70 +0.3 1,542,394 - MMJJ_RS08360 0.70 +1.1 1,542,420 - MMJJ_RS08360 0.72 +1.4 1,542,421 + MMJJ_RS08360 0.72 +0.0 1,542,421 + MMJJ_RS08360 0.72 +2.7 1,542,522 - MMJJ_RS08360 0.81 -1.9 1,542,532 - MMJJ_RS08360 0.82 +2.4 1,542,533 + MMJJ_RS08360 0.82 +1.5 1,542,533 + MMJJ_RS08360 0.82 +0.4 1,542,533 + MMJJ_RS08360 0.82 +0.6 1,542,605 - MMJJ_RS08360 0.88 -0.6 1,542,606 + MMJJ_RS08360 0.88 +0.4 1,542,606 + MMJJ_RS08360 0.88 -0.4 1,542,610 + MMJJ_RS08360 0.88 +1.1 1,542,612 - MMJJ_RS08360 0.88 +0.5 1,542,612 - MMJJ_RS08360 0.88 +1.0 1,542,612 - MMJJ_RS08360 0.88 -0.4 1,542,635 - +0.8 1,542,636 + +1.5 1,542,640 - -1.0 1,542,674 + -0.5 1,542,718 - -1.2 1,542,733 + +2.5 1,542,737 - +0.2 1,542,751 - -0.1 1,542,758 + +1.0 1,542,780 - +1.0 1,542,781 + +2.2 1,542,781 + -1.2 1,542,781 + +0.6 1,542,785 - +0.7 1,542,877 + MMJJ_RS08365 0.26 +1.1 1,542,877 + MMJJ_RS08365 0.26 +2.3 1,542,877 + MMJJ_RS08365 0.26 +1.6 1,542,879 - MMJJ_RS08365 0.27 -0.7 1,542,879 - MMJJ_RS08365 0.27 -0.6 1,542,879 - MMJJ_RS08365 0.27 +0.6 1,542,880 + MMJJ_RS08365 0.27 +0.1 1,542,880 + MMJJ_RS08365 0.27 +0.2 1,542,884 + MMJJ_RS08365 0.28 -0.3 1,542,897 - -0.2 1,542,900 - MMJJ_RS08365 0.33 +0.2 1,543,022 + MMJJ_RS08365 0.68 +2.2 1,543,073 + MMJJ_RS08365 0.82 +1.3 1,543,085 - MMJJ_RS08365 0.86 +1.4 1,543,102 + -0.0 1,543,107 + +2.8 1,543,110 - -1.6 1,543,110 - -0.3 1,543,110 - -0.2 1,543,111 + +2.6 1,543,111 + +2.6 1,543,111 + +0.1 1,543,159 - -0.4 1,543,160 + +2.6 1,543,171 - +0.9 1,543,171 - +2.5 1,543,173 - -0.2 1,543,241 - MMJJ_RS08370 0.38 +1.1 1,543,241 - MMJJ_RS08370 0.38 -0.8 1,543,252 + MMJJ_RS08370 0.43 +1.7 1,543,252 + MMJJ_RS08370 0.43 +0.3 1,543,323 - MMJJ_RS08370 0.81 +0.4 1,543,323 - MMJJ_RS08370 0.81 +0.7 1,543,324 + MMJJ_RS08370 0.81 +0.4 1,543,324 + MMJJ_RS08370 0.81 +0.7 1,543,324 + MMJJ_RS08370 0.81 +2.3 1,543,327 - MMJJ_RS08370 0.83 +1.9 1,543,353 - -0.5 1,543,353 - -0.1 1,543,358 - +1.1 1,543,358 - -0.4 1,543,359 + +2.8 1,543,359 + -0.9 1,543,359 + -1.2 1,543,378 - -0.9 1,543,379 + +0.1 1,543,385 - -0.0 1,543,447 - MMJJ_RS08375 0.14 -1.0 1,543,448 + MMJJ_RS08375 0.14 -0.9 1,543,452 - MMJJ_RS08375 0.15 +0.1 1,543,452 - MMJJ_RS08375 0.15 -0.5 1,543,453 + MMJJ_RS08375 0.15 +1.3 1,543,453 + MMJJ_RS08375 0.15 -0.1 1,543,453 + MMJJ_RS08375 0.15 +0.7 1,543,453 + MMJJ_RS08375 0.15 -0.0 1,543,453 + MMJJ_RS08375 0.15 +0.6 1,543,467 - MMJJ_RS08375 0.17 +1.0 1,543,524 - MMJJ_RS08375 0.28 +1.1 1,543,556 + MMJJ_RS08375 0.35 +1.2 1,543,564 + MMJJ_RS08375 0.36 -1.2 1,543,609 - MMJJ_RS08375 0.45 +0.0 1,543,610 + MMJJ_RS08375 0.45 +0.9 1,543,610 + MMJJ_RS08375 0.45 +0.1 1,543,623 + MMJJ_RS08375 0.48 -0.4 1,543,644 - MMJJ_RS08375 0.52 -0.0 1,543,644 - MMJJ_RS08375 0.52 +1.4 1,543,644 - MMJJ_RS08375 0.52 +0.5 1,543,645 + MMJJ_RS08375 0.52 +0.2 1,543,645 + MMJJ_RS08375 0.52 +0.3 1,543,646 - MMJJ_RS08375 0.52 +1.3 1,543,647 + MMJJ_RS08375 0.52 +2.0 1,543,650 + MMJJ_RS08375 0.53 +0.4 1,543,713 - MMJJ_RS08375 0.65 -0.2 1,543,713 - MMJJ_RS08375 0.65 -0.9 1,543,713 - MMJJ_RS08375 0.65 -0.6 1,543,714 + MMJJ_RS08375 0.65 +0.0 1,543,714 + MMJJ_RS08375 0.65 +1.3 1,543,714 + MMJJ_RS08375 0.65 -0.2 1,543,717 - MMJJ_RS08375 0.66 +0.1 1,543,717 - MMJJ_RS08375 0.66 -0.2 1,543,718 + MMJJ_RS08375 0.66 +0.8 1,543,807 - MMJJ_RS08375 0.83 -0.9 1,543,808 + MMJJ_RS08375 0.84 +1.3 1,543,827 - MMJJ_RS08375 0.87 -0.5 1,543,827 - MMJJ_RS08375 0.87 -0.1 1,543,827 - MMJJ_RS08375 0.87 -0.4 1,543,827 - MMJJ_RS08375 0.87 +1.4 1,543,827 - MMJJ_RS08375 0.87 -2.1 1,543,827 - MMJJ_RS08375 0.87 +0.6 1,543,827 - MMJJ_RS08375 0.87 -0.6 1,543,828 + MMJJ_RS08375 0.87 +0.8 1,543,828 + MMJJ_RS08375 0.87 +1.6 1,543,828 + MMJJ_RS08375 0.87 -1.0 1,543,831 - MMJJ_RS08375 0.88 -0.1 1,543,831 - MMJJ_RS08375 0.88 +0.0 1,543,831 - MMJJ_RS08375 0.88 -0.9 1,543,831 - MMJJ_RS08375 0.88 -0.2 1,543,832 + MMJJ_RS08375 0.88 -0.4 1,543,840 - MMJJ_RS08375 0.90 -2.6 1,543,841 + +0.9 1,543,866 - +0.6 1,543,867 + +0.8 1,543,878 - -1.2 1,543,879 + +0.1 1,543,879 + +1.8 1,543,879 + -0.7 1,543,879 + -0.8 1,543,883 - +1.9 1,543,892 - -0.4 1,543,909 - -0.5 1,543,909 - -0.2 1,543,910 + +0.6 1,543,920 - -0.1 1,543,921 + -0.7 1,543,921 + -0.1 1,543,936 + +1.4 1,543,990 - MMJJ_RS08380 0.28 +0.4 1,544,018 + -0.6 1,544,018 + MMJJ_RS08380 0.39 +0.0 1,544,018 + MMJJ_RS08380 0.39 +0.9 1,544,090 + MMJJ_RS08380 0.66 +0.1
Or see this region's nucleotide sequence