Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02105 and MMJJ_RS02110 are separated by 273 nucleotides MMJJ_RS02110 and MMJJ_RS02115 are separated by 11 nucleotides MMJJ_RS02115 and MMJJ_RS02120 are separated by 61 nucleotides MMJJ_RS02120 and MMJJ_RS02125 are separated by 118 nucleotides
MMJJ_RS02105: MMJJ_RS02105 - right-handed parallel beta-helix repeat-containing protein, at 400,125 to 403,277
_RS02105
MMJJ_RS02110: MMJJ_RS02110 - carboxymuconolactone decarboxylase family protein, at 403,551 to 403,985
_RS02110
MMJJ_RS02115: MMJJ_RS02115 - class I SAM-dependent methyltransferase family protein, at 403,997 to 404,758
_RS02115
MMJJ_RS02120: MMJJ_RS02120 - DJ-1/PfpI family protein, at 404,820 to 405,335
_RS02120
MMJJ_RS02125: MMJJ_RS02125 - hypothetical protein, at 405,454 to 405,750
_RS02125
Position (kb) 403
404
405 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 403.003 kb on - strand at 403.005 kb on - strand at 403.006 kb on + strand at 403.006 kb on + strand at 403.011 kb on + strand at 403.019 kb on - strand at 403.019 kb on - strand at 403.020 kb on + strand at 403.029 kb on + strand at 403.050 kb on - strand at 403.068 kb on + strand at 403.101 kb on - strand at 403.101 kb on - strand at 403.122 kb on - strand at 403.153 kb on + strand at 403.157 kb on - strand at 403.159 kb on - strand at 403.159 kb on - strand at 403.160 kb on + strand at 403.161 kb on - strand at 403.162 kb on + strand at 403.176 kb on - strand at 403.195 kb on - strand at 403.199 kb on - strand at 403.205 kb on - strand at 403.206 kb on + strand at 403.210 kb on + strand at 403.210 kb on + strand at 403.210 kb on + strand at 403.210 kb on + strand at 403.210 kb on + strand at 403.210 kb on + strand at 403.211 kb on - strand at 403.222 kb on + strand at 403.254 kb on + strand at 403.299 kb on - strand at 403.299 kb on - strand at 403.300 kb on + strand at 403.300 kb on + strand at 403.301 kb on - strand at 403.301 kb on - strand at 403.357 kb on - strand at 403.360 kb on + strand at 403.362 kb on + strand at 403.373 kb on - strand at 403.437 kb on + strand at 403.453 kb on + strand at 403.464 kb on - strand at 403.464 kb on - strand at 403.474 kb on + strand at 403.474 kb on + strand at 403.478 kb on - strand at 403.478 kb on - strand at 403.479 kb on + strand at 403.484 kb on + strand at 403.518 kb on - strand at 403.523 kb on - strand at 403.530 kb on + strand at 403.538 kb on - strand at 403.538 kb on - strand at 403.538 kb on - strand at 403.538 kb on - strand at 403.539 kb on + strand at 403.539 kb on + strand at 403.539 kb on + strand at 403.545 kb on - strand at 403.545 kb on - strand at 403.546 kb on + strand at 403.548 kb on + strand at 403.550 kb on - strand at 403.551 kb on + strand at 403.551 kb on + strand at 403.551 kb on + strand at 403.595 kb on + strand, within MMJJ_RS02110 at 403.710 kb on - strand, within MMJJ_RS02110 at 403.710 kb on - strand, within MMJJ_RS02110 at 403.711 kb on + strand, within MMJJ_RS02110 at 403.719 kb on + strand, within MMJJ_RS02110 at 403.748 kb on - strand, within MMJJ_RS02110 at 403.809 kb on + strand, within MMJJ_RS02110 at 403.823 kb on + strand, within MMJJ_RS02110 at 403.871 kb on - strand, within MMJJ_RS02110 at 403.872 kb on + strand, within MMJJ_RS02110 at 403.920 kb on + strand, within MMJJ_RS02110 at 403.928 kb on + strand, within MMJJ_RS02110 at 403.950 kb on + strand at 404.024 kb on - strand at 404.024 kb on - strand at 404.050 kb on + strand at 404.066 kb on + strand at 404.072 kb on + strand at 404.072 kb on + strand at 404.074 kb on + strand, within MMJJ_RS02115 at 404.191 kb on - strand, within MMJJ_RS02115 at 404.324 kb on - strand, within MMJJ_RS02115 at 404.379 kb on - strand, within MMJJ_RS02115 at 404.380 kb on + strand, within MMJJ_RS02115 at 404.444 kb on + strand, within MMJJ_RS02115 at 404.450 kb on - strand, within MMJJ_RS02115 at 404.451 kb on + strand, within MMJJ_RS02115 at 404.477 kb on + strand, within MMJJ_RS02115 at 404.480 kb on - strand, within MMJJ_RS02115 at 404.520 kb on + strand, within MMJJ_RS02115 at 404.528 kb on - strand, within MMJJ_RS02115 at 404.529 kb on + strand, within MMJJ_RS02115 at 404.626 kb on - strand, within MMJJ_RS02115 at 404.627 kb on + strand, within MMJJ_RS02115 at 404.627 kb on + strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.628 kb on - strand, within MMJJ_RS02115 at 404.629 kb on + strand, within MMJJ_RS02115 at 404.629 kb on + strand, within MMJJ_RS02115 at 404.629 kb on + strand, within MMJJ_RS02115 at 404.632 kb on - strand, within MMJJ_RS02115 at 404.633 kb on + strand, within MMJJ_RS02115 at 404.633 kb on + strand, within MMJJ_RS02115 at 404.633 kb on + strand, within MMJJ_RS02115 at 404.633 kb on + strand, within MMJJ_RS02115 at 404.636 kb on + strand, within MMJJ_RS02115 at 404.643 kb on + strand, within MMJJ_RS02115 at 404.647 kb on - strand, within MMJJ_RS02115 at 404.647 kb on - strand, within MMJJ_RS02115 at 404.665 kb on - strand, within MMJJ_RS02115 at 404.769 kb on - strand at 404.775 kb on + strand at 404.777 kb on + strand at 404.785 kb on + strand at 404.817 kb on - strand at 404.818 kb on + strand at 404.818 kb on + strand at 404.821 kb on - strand at 404.889 kb on - strand, within MMJJ_RS02120 at 405.062 kb on - strand, within MMJJ_RS02120 at 405.107 kb on + strand, within MMJJ_RS02120 at 405.134 kb on - strand, within MMJJ_RS02120 at 405.138 kb on - strand, within MMJJ_RS02120 at 405.138 kb on - strand, within MMJJ_RS02120 at 405.138 kb on - strand, within MMJJ_RS02120 at 405.139 kb on + strand, within MMJJ_RS02120 at 405.139 kb on + strand, within MMJJ_RS02120 at 405.325 kb on + strand at 405.361 kb on + strand at 405.361 kb on + strand at 405.364 kb on - strand at 405.364 kb on - strand at 405.365 kb on + strand at 405.365 kb on + strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.372 kb on - strand at 405.372 kb on - strand at 405.373 kb on + strand at 405.373 kb on + strand at 405.374 kb on - strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.379 kb on - strand at 405.385 kb on + strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.426 kb on - strand at 405.427 kb on + strand at 405.442 kb on + strand at 405.513 kb on - strand, within MMJJ_RS02125 at 405.513 kb on - strand, within MMJJ_RS02125 at 405.513 kb on - strand, within MMJJ_RS02125 at 405.514 kb on + strand, within MMJJ_RS02125 at 405.583 kb on - strand, within MMJJ_RS02125 at 405.594 kb on - strand, within MMJJ_RS02125 at 405.749 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 403,003 - -0.1 403,005 - +1.8 403,006 + -0.6 403,006 + +0.3 403,011 + -0.7 403,019 - -1.0 403,019 - +0.8 403,020 + +0.1 403,029 + +2.5 403,050 - +2.3 403,068 + +0.4 403,101 - -0.6 403,101 - +0.8 403,122 - +0.3 403,153 + +0.6 403,157 - +0.1 403,159 - +0.3 403,159 - -0.3 403,160 + -1.3 403,161 - +1.2 403,162 + +0.1 403,176 - +0.4 403,195 - +0.7 403,199 - -0.9 403,205 - +0.6 403,206 + -0.4 403,210 + +0.2 403,210 + -0.8 403,210 + -0.8 403,210 + +1.1 403,210 + -1.0 403,210 + -0.8 403,211 - -0.9 403,222 + -0.9 403,254 + +0.5 403,299 - +0.3 403,299 - +1.7 403,300 + -0.4 403,300 + +0.8 403,301 - -1.0 403,301 - -0.3 403,357 - -0.3 403,360 + -0.2 403,362 + -0.6 403,373 - -0.3 403,437 + -0.8 403,453 + +0.5 403,464 - +0.7 403,464 - +0.1 403,474 + +0.9 403,474 + -1.0 403,478 - -2.1 403,478 - -0.8 403,479 + +1.3 403,484 + +0.5 403,518 - -0.4 403,523 - -0.6 403,530 + -0.5 403,538 - +0.5 403,538 - -1.7 403,538 - +0.0 403,538 - +0.5 403,539 + +0.8 403,539 + +0.5 403,539 + +1.7 403,545 - +0.0 403,545 - +1.0 403,546 + +0.3 403,548 + +0.4 403,550 - -0.6 403,551 + +0.4 403,551 + +0.3 403,551 + -0.2 403,595 + MMJJ_RS02110 0.10 -0.3 403,710 - MMJJ_RS02110 0.37 +1.0 403,710 - MMJJ_RS02110 0.37 +0.6 403,711 + MMJJ_RS02110 0.37 +1.3 403,719 + MMJJ_RS02110 0.39 -0.7 403,748 - MMJJ_RS02110 0.45 +0.2 403,809 + MMJJ_RS02110 0.59 +0.1 403,823 + MMJJ_RS02110 0.63 +0.0 403,871 - MMJJ_RS02110 0.74 -0.1 403,872 + MMJJ_RS02110 0.74 -0.8 403,920 + MMJJ_RS02110 0.85 -1.8 403,928 + MMJJ_RS02110 0.87 -0.7 403,950 + +0.5 404,024 - -0.4 404,024 - -0.8 404,050 + +1.1 404,066 + -0.7 404,072 + -0.7 404,072 + -0.6 404,074 + MMJJ_RS02115 0.10 -0.7 404,191 - MMJJ_RS02115 0.25 +1.7 404,324 - MMJJ_RS02115 0.43 -1.5 404,379 - MMJJ_RS02115 0.50 -0.6 404,380 + MMJJ_RS02115 0.50 +0.7 404,444 + MMJJ_RS02115 0.59 +0.6 404,450 - MMJJ_RS02115 0.59 +0.9 404,451 + MMJJ_RS02115 0.60 +0.7 404,477 + MMJJ_RS02115 0.63 +2.0 404,480 - MMJJ_RS02115 0.63 +2.0 404,520 + MMJJ_RS02115 0.69 -1.8 404,528 - MMJJ_RS02115 0.70 +0.9 404,529 + MMJJ_RS02115 0.70 -0.1 404,626 - MMJJ_RS02115 0.83 +0.9 404,627 + MMJJ_RS02115 0.83 +2.1 404,627 + MMJJ_RS02115 0.83 +1.0 404,628 - MMJJ_RS02115 0.83 +0.2 404,628 - MMJJ_RS02115 0.83 +0.5 404,628 - MMJJ_RS02115 0.83 -0.5 404,628 - MMJJ_RS02115 0.83 +1.3 404,628 - MMJJ_RS02115 0.83 -0.7 404,628 - MMJJ_RS02115 0.83 -1.7 404,628 - MMJJ_RS02115 0.83 +2.0 404,628 - MMJJ_RS02115 0.83 -0.7 404,628 - MMJJ_RS02115 0.83 +1.5 404,628 - MMJJ_RS02115 0.83 +0.4 404,629 + MMJJ_RS02115 0.83 +0.3 404,629 + MMJJ_RS02115 0.83 +1.6 404,629 + MMJJ_RS02115 0.83 +1.8 404,632 - MMJJ_RS02115 0.83 +0.2 404,633 + MMJJ_RS02115 0.83 -0.4 404,633 + MMJJ_RS02115 0.83 -0.0 404,633 + MMJJ_RS02115 0.83 +1.5 404,633 + MMJJ_RS02115 0.83 +1.1 404,636 + MMJJ_RS02115 0.84 -0.8 404,643 + MMJJ_RS02115 0.85 -0.5 404,647 - MMJJ_RS02115 0.85 -1.0 404,647 - MMJJ_RS02115 0.85 +0.3 404,665 - MMJJ_RS02115 0.88 -0.3 404,769 - +1.9 404,775 + +1.3 404,777 + +0.5 404,785 + +1.1 404,817 - -0.8 404,818 + -0.7 404,818 + +3.0 404,821 - +0.6 404,889 - MMJJ_RS02120 0.13 +0.6 405,062 - MMJJ_RS02120 0.47 +1.7 405,107 + MMJJ_RS02120 0.56 +0.1 405,134 - MMJJ_RS02120 0.61 +0.3 405,138 - MMJJ_RS02120 0.62 +0.5 405,138 - MMJJ_RS02120 0.62 -1.0 405,138 - MMJJ_RS02120 0.62 +0.6 405,139 + MMJJ_RS02120 0.62 -0.7 405,139 + MMJJ_RS02120 0.62 +1.5 405,325 + +0.8 405,361 + +1.4 405,361 + -0.3 405,364 - +0.1 405,364 - -1.7 405,365 + +2.0 405,365 + -1.8 405,370 - -0.0 405,370 - +0.4 405,370 - -0.3 405,370 - +1.5 405,370 - -0.7 405,370 - +0.2 405,370 - +0.9 405,370 - -1.6 405,370 - +0.6 405,370 - +1.5 405,370 - +0.4 405,370 - -0.0 405,370 - +0.6 405,371 + -1.3 405,371 + +0.4 405,371 + -0.0 405,371 + -2.1 405,371 + -0.5 405,371 + -1.4 405,371 + -0.0 405,371 + +1.0 405,371 + +2.1 405,371 + -0.6 405,371 + +0.6 405,371 + -1.0 405,371 + -0.3 405,371 + +1.5 405,371 + -0.0 405,371 + +0.3 405,371 + +1.8 405,371 + +0.7 405,372 - +1.6 405,372 - -0.5 405,373 + +0.2 405,373 + -2.4 405,374 - +1.3 405,375 + -1.8 405,375 + -0.8 405,375 + +0.9 405,375 + -1.9 405,379 - -0.4 405,385 + -0.3 405,398 - -0.1 405,398 - -0.6 405,398 - +0.5 405,398 - -1.5 405,426 - +2.1 405,427 + -1.0 405,442 + +1.1 405,513 - MMJJ_RS02125 0.20 +0.4 405,513 - MMJJ_RS02125 0.20 +1.3 405,513 - MMJJ_RS02125 0.20 -0.8 405,514 + MMJJ_RS02125 0.20 +0.5 405,583 - MMJJ_RS02125 0.43 +1.1 405,594 - MMJJ_RS02125 0.47 -0.8 405,749 + -3.3
Or see this region's nucleotide sequence