Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01255 and MMJJ_RS01260 are separated by 8 nucleotides MMJJ_RS01260 and MMJJ_RS01265 are separated by 228 nucleotides MMJJ_RS01265 and MMJJ_RS01270 are separated by 17 nucleotides MMJJ_RS01270 and MMJJ_RS01275 are separated by 40 nucleotides
MMJJ_RS01255: MMJJ_RS01255 - YqhA family protein, at 243,249 to 243,806
_RS01255
MMJJ_RS01260: MMJJ_RS01260 - hypothetical protein, at 243,815 to 244,159
_RS01260
MMJJ_RS01265: MMJJ_RS01265 - cupin domain-containing protein, at 244,388 to 244,732
_RS01265
MMJJ_RS01270: MMJJ_RS01270 - 4Fe-4S binding protein, at 244,750 to 245,382
_RS01270
MMJJ_RS01275: MMJJ_RS01275 - 4Fe-4S dicluster domain-containing protein, at 245,423 to 246,121
_RS01275
Position (kb) 244
245 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 243.395 kb on - strand, within MMJJ_RS01255 at 243.395 kb on - strand, within MMJJ_RS01255 at 243.441 kb on + strand, within MMJJ_RS01255 at 243.459 kb on + strand, within MMJJ_RS01255 at 243.485 kb on + strand, within MMJJ_RS01255 at 243.487 kb on - strand, within MMJJ_RS01255 at 243.509 kb on + strand, within MMJJ_RS01255 at 243.520 kb on - strand, within MMJJ_RS01255 at 243.523 kb on - strand, within MMJJ_RS01255 at 243.532 kb on - strand, within MMJJ_RS01255 at 243.533 kb on + strand, within MMJJ_RS01255 at 243.545 kb on - strand, within MMJJ_RS01255 at 243.559 kb on + strand, within MMJJ_RS01255 at 243.559 kb on + strand, within MMJJ_RS01255 at 243.580 kb on - strand, within MMJJ_RS01255 at 243.615 kb on + strand, within MMJJ_RS01255 at 243.628 kb on + strand, within MMJJ_RS01255 at 243.629 kb on - strand, within MMJJ_RS01255 at 243.629 kb on - strand, within MMJJ_RS01255 at 243.665 kb on - strand, within MMJJ_RS01255 at 243.667 kb on - strand, within MMJJ_RS01255 at 243.668 kb on + strand, within MMJJ_RS01255 at 243.668 kb on + strand, within MMJJ_RS01255 at 243.671 kb on + strand, within MMJJ_RS01255 at 243.679 kb on - strand, within MMJJ_RS01255 at 243.679 kb on - strand, within MMJJ_RS01255 at 243.730 kb on - strand, within MMJJ_RS01255 at 243.734 kb on - strand, within MMJJ_RS01255 at 243.735 kb on + strand, within MMJJ_RS01255 at 243.739 kb on - strand, within MMJJ_RS01255 at 243.739 kb on - strand, within MMJJ_RS01255 at 243.740 kb on + strand, within MMJJ_RS01255 at 243.740 kb on + strand, within MMJJ_RS01255 at 243.740 kb on + strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.743 kb on - strand, within MMJJ_RS01255 at 243.743 kb on - strand, within MMJJ_RS01255 at 243.743 kb on - strand, within MMJJ_RS01255 at 243.744 kb on + strand, within MMJJ_RS01255 at 243.744 kb on + strand, within MMJJ_RS01255 at 243.805 kb on + strand at 243.820 kb on + strand at 243.820 kb on + strand at 243.834 kb on + strand at 243.863 kb on + strand, within MMJJ_RS01260 at 243.926 kb on - strand, within MMJJ_RS01260 at 243.927 kb on + strand, within MMJJ_RS01260 at 243.961 kb on - strand, within MMJJ_RS01260 at 243.962 kb on + strand, within MMJJ_RS01260 at 243.962 kb on + strand, within MMJJ_RS01260 at 243.962 kb on + strand, within MMJJ_RS01260 at 243.968 kb on + strand, within MMJJ_RS01260 at 243.971 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.991 kb on - strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 244.021 kb on - strand, within MMJJ_RS01260 at 244.026 kb on - strand, within MMJJ_RS01260 at 244.038 kb on - strand, within MMJJ_RS01260 at 244.039 kb on + strand, within MMJJ_RS01260 at 244.039 kb on + strand, within MMJJ_RS01260 at 244.067 kb on + strand, within MMJJ_RS01260 at 244.084 kb on + strand, within MMJJ_RS01260 at 244.084 kb on + strand, within MMJJ_RS01260 at 244.204 kb on - strand at 244.204 kb on - strand at 244.212 kb on - strand at 244.213 kb on + strand at 244.219 kb on + strand at 244.239 kb on - strand at 244.265 kb on - strand at 244.303 kb on - strand at 244.308 kb on - strand at 244.309 kb on + strand at 244.331 kb on - strand at 244.331 kb on - strand at 244.332 kb on + strand at 244.332 kb on + strand at 244.332 kb on + strand at 244.332 kb on + strand at 244.332 kb on + strand at 244.333 kb on - strand at 244.334 kb on + strand at 244.338 kb on + strand at 244.347 kb on - strand at 244.347 kb on - strand at 244.348 kb on + strand at 244.352 kb on + strand at 244.354 kb on - strand at 244.373 kb on + strand at 244.375 kb on + strand at 244.397 kb on + strand at 244.397 kb on + strand at 244.397 kb on + strand at 244.397 kb on + strand at 244.506 kb on + strand, within MMJJ_RS01265 at 244.506 kb on + strand, within MMJJ_RS01265 at 244.506 kb on + strand, within MMJJ_RS01265 at 244.511 kb on - strand, within MMJJ_RS01265 at 244.583 kb on - strand, within MMJJ_RS01265 at 244.584 kb on + strand, within MMJJ_RS01265 at 244.644 kb on + strand, within MMJJ_RS01265 at 244.644 kb on + strand, within MMJJ_RS01265 at 244.652 kb on - strand, within MMJJ_RS01265 at 244.674 kb on + strand, within MMJJ_RS01265 at 244.674 kb on + strand, within MMJJ_RS01265 at 244.735 kb on - strand at 244.763 kb on - strand at 244.763 kb on - strand at 244.768 kb on - strand at 244.789 kb on - strand at 244.876 kb on - strand, within MMJJ_RS01270 at 244.893 kb on - strand, within MMJJ_RS01270 at 244.893 kb on - strand, within MMJJ_RS01270 at 244.903 kb on + strand, within MMJJ_RS01270 at 244.928 kb on - strand, within MMJJ_RS01270 at 244.928 kb on - strand, within MMJJ_RS01270 at 245.013 kb on - strand, within MMJJ_RS01270 at 245.040 kb on - strand, within MMJJ_RS01270 at 245.107 kb on - strand, within MMJJ_RS01270 at 245.108 kb on + strand, within MMJJ_RS01270 at 245.143 kb on - strand, within MMJJ_RS01270 at 245.181 kb on - strand, within MMJJ_RS01270 at 245.197 kb on - strand, within MMJJ_RS01270 at 245.197 kb on - strand, within MMJJ_RS01270 at 245.208 kb on - strand, within MMJJ_RS01270 at 245.215 kb on + strand, within MMJJ_RS01270 at 245.218 kb on - strand, within MMJJ_RS01270 at 245.244 kb on + strand, within MMJJ_RS01270 at 245.244 kb on + strand, within MMJJ_RS01270 at 245.244 kb on + strand, within MMJJ_RS01270 at 245.267 kb on + strand, within MMJJ_RS01270 at 245.317 kb on + strand, within MMJJ_RS01270 at 245.467 kb on - strand at 245.472 kb on + strand at 245.472 kb on + strand at 245.477 kb on - strand at 245.550 kb on + strand, within MMJJ_RS01275 at 245.630 kb on - strand, within MMJJ_RS01275 at 245.630 kb on - strand, within MMJJ_RS01275 at 245.722 kb on - strand, within MMJJ_RS01275 at 245.727 kb on - strand, within MMJJ_RS01275 at 245.727 kb on - strand, within MMJJ_RS01275 at 245.728 kb on + strand, within MMJJ_RS01275
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 243,395 - MMJJ_RS01255 0.26 -1.3 243,395 - MMJJ_RS01255 0.26 +0.8 243,441 + MMJJ_RS01255 0.34 -1.3 243,459 + MMJJ_RS01255 0.38 -0.3 243,485 + MMJJ_RS01255 0.42 +0.1 243,487 - MMJJ_RS01255 0.43 +0.2 243,509 + MMJJ_RS01255 0.47 -1.0 243,520 - MMJJ_RS01255 0.49 -1.1 243,523 - MMJJ_RS01255 0.49 -1.0 243,532 - MMJJ_RS01255 0.51 +0.9 243,533 + MMJJ_RS01255 0.51 +0.9 243,545 - MMJJ_RS01255 0.53 +0.8 243,559 + MMJJ_RS01255 0.56 +0.9 243,559 + MMJJ_RS01255 0.56 +0.9 243,580 - MMJJ_RS01255 0.59 +2.2 243,615 + MMJJ_RS01255 0.66 +0.0 243,628 + MMJJ_RS01255 0.68 -0.9 243,629 - MMJJ_RS01255 0.68 -0.4 243,629 - MMJJ_RS01255 0.68 +1.8 243,665 - MMJJ_RS01255 0.75 +2.2 243,667 - MMJJ_RS01255 0.75 +1.5 243,668 + MMJJ_RS01255 0.75 -1.9 243,668 + MMJJ_RS01255 0.75 -1.0 243,671 + MMJJ_RS01255 0.76 -1.1 243,679 - MMJJ_RS01255 0.77 -2.0 243,679 - MMJJ_RS01255 0.77 -0.1 243,730 - MMJJ_RS01255 0.86 -2.4 243,734 - MMJJ_RS01255 0.87 -1.4 243,735 + MMJJ_RS01255 0.87 +1.8 243,739 - MMJJ_RS01255 0.88 -2.4 243,739 - MMJJ_RS01255 0.88 -1.8 243,740 + MMJJ_RS01255 0.88 -2.9 243,740 + MMJJ_RS01255 0.88 -1.6 243,740 + MMJJ_RS01255 0.88 -0.8 243,741 - MMJJ_RS01255 0.88 -4.7 243,741 - MMJJ_RS01255 0.88 -3.3 243,741 - MMJJ_RS01255 0.88 -2.5 243,741 - MMJJ_RS01255 0.88 -4.0 243,741 - MMJJ_RS01255 0.88 -3.8 243,741 - MMJJ_RS01255 0.88 -4.8 243,741 - MMJJ_RS01255 0.88 -0.3 243,742 + MMJJ_RS01255 0.88 -3.7 243,742 + MMJJ_RS01255 0.88 -3.5 243,742 + MMJJ_RS01255 0.88 +0.3 243,742 + MMJJ_RS01255 0.88 -3.1 243,742 + MMJJ_RS01255 0.88 -3.3 243,742 + MMJJ_RS01255 0.88 -3.8 243,742 + MMJJ_RS01255 0.88 -3.2 243,742 + MMJJ_RS01255 0.88 -2.7 243,742 + MMJJ_RS01255 0.88 -2.4 243,742 + MMJJ_RS01255 0.88 -4.5 243,742 + MMJJ_RS01255 0.88 -2.6 243,742 + MMJJ_RS01255 0.88 -2.6 243,742 + MMJJ_RS01255 0.88 -3.4 243,742 + MMJJ_RS01255 0.88 -1.8 243,742 + MMJJ_RS01255 0.88 -3.2 243,743 - MMJJ_RS01255 0.89 -0.5 243,743 - MMJJ_RS01255 0.89 +0.3 243,743 - MMJJ_RS01255 0.89 -1.0 243,744 + MMJJ_RS01255 0.89 +1.0 243,744 + MMJJ_RS01255 0.89 +1.0 243,805 + -0.6 243,820 + +0.3 243,820 + +0.8 243,834 + -0.5 243,863 + MMJJ_RS01260 0.14 +1.2 243,926 - MMJJ_RS01260 0.32 -0.8 243,927 + MMJJ_RS01260 0.32 +2.0 243,961 - MMJJ_RS01260 0.42 +0.7 243,962 + MMJJ_RS01260 0.43 -0.7 243,962 + MMJJ_RS01260 0.43 -0.0 243,962 + MMJJ_RS01260 0.43 -0.8 243,968 + MMJJ_RS01260 0.44 -0.1 243,971 - MMJJ_RS01260 0.45 +1.8 243,989 - MMJJ_RS01260 0.50 +0.5 243,989 - MMJJ_RS01260 0.50 -0.7 243,989 - MMJJ_RS01260 0.50 -0.4 243,989 - MMJJ_RS01260 0.50 +1.6 243,989 - MMJJ_RS01260 0.50 -1.4 243,990 + MMJJ_RS01260 0.51 -1.1 243,990 + MMJJ_RS01260 0.51 -0.2 243,990 + MMJJ_RS01260 0.51 +1.5 243,990 + MMJJ_RS01260 0.51 -0.6 243,990 + MMJJ_RS01260 0.51 +0.3 243,990 + MMJJ_RS01260 0.51 -1.6 243,990 + MMJJ_RS01260 0.51 +1.0 243,990 + MMJJ_RS01260 0.51 +0.0 243,991 - MMJJ_RS01260 0.51 +0.7 243,992 + MMJJ_RS01260 0.51 +0.5 243,992 + MMJJ_RS01260 0.51 -0.6 243,992 + MMJJ_RS01260 0.51 -0.2 243,992 + MMJJ_RS01260 0.51 -0.9 243,992 + MMJJ_RS01260 0.51 +0.7 244,021 - MMJJ_RS01260 0.60 -0.2 244,026 - MMJJ_RS01260 0.61 +1.1 244,038 - MMJJ_RS01260 0.65 -2.2 244,039 + MMJJ_RS01260 0.65 +0.5 244,039 + MMJJ_RS01260 0.65 +0.6 244,067 + MMJJ_RS01260 0.73 -0.7 244,084 + MMJJ_RS01260 0.78 -0.5 244,084 + MMJJ_RS01260 0.78 -0.8 244,204 - +2.5 244,204 - +0.7 244,212 - +2.6 244,213 + +0.1 244,219 + -0.8 244,239 - +0.9 244,265 - -0.7 244,303 - -1.0 244,308 - +0.4 244,309 + -0.9 244,331 - +0.6 244,331 - -0.3 244,332 + +1.0 244,332 + +0.4 244,332 + +1.5 244,332 + +0.2 244,332 + +1.3 244,333 - +0.3 244,334 + +0.7 244,338 + +2.3 244,347 - -0.9 244,347 - +0.3 244,348 + -0.5 244,352 + +0.4 244,354 - +0.4 244,373 + -0.2 244,375 + +0.5 244,397 + +1.7 244,397 + +1.3 244,397 + +1.9 244,397 + +2.4 244,506 + MMJJ_RS01265 0.34 +0.9 244,506 + MMJJ_RS01265 0.34 -1.2 244,506 + MMJJ_RS01265 0.34 +1.2 244,511 - MMJJ_RS01265 0.36 -0.0 244,583 - MMJJ_RS01265 0.57 +2.3 244,584 + MMJJ_RS01265 0.57 +0.1 244,644 + MMJJ_RS01265 0.74 -1.6 244,644 + MMJJ_RS01265 0.74 +0.3 244,652 - MMJJ_RS01265 0.77 -0.0 244,674 + MMJJ_RS01265 0.83 +1.1 244,674 + MMJJ_RS01265 0.83 +0.2 244,735 - +0.8 244,763 - +0.2 244,763 - +1.3 244,768 - -1.5 244,789 - -0.6 244,876 - MMJJ_RS01270 0.20 +0.5 244,893 - MMJJ_RS01270 0.23 +0.2 244,893 - MMJJ_RS01270 0.23 -0.6 244,903 + MMJJ_RS01270 0.24 -0.8 244,928 - MMJJ_RS01270 0.28 +2.2 244,928 - MMJJ_RS01270 0.28 +1.8 245,013 - MMJJ_RS01270 0.42 +0.5 245,040 - MMJJ_RS01270 0.46 -0.8 245,107 - MMJJ_RS01270 0.56 -1.3 245,108 + MMJJ_RS01270 0.57 -0.1 245,143 - MMJJ_RS01270 0.62 -0.6 245,181 - MMJJ_RS01270 0.68 -1.5 245,197 - MMJJ_RS01270 0.71 -0.5 245,197 - MMJJ_RS01270 0.71 +0.1 245,208 - MMJJ_RS01270 0.72 +1.5 245,215 + MMJJ_RS01270 0.73 +2.1 245,218 - MMJJ_RS01270 0.74 -1.3 245,244 + MMJJ_RS01270 0.78 -0.9 245,244 + MMJJ_RS01270 0.78 -1.2 245,244 + MMJJ_RS01270 0.78 +0.1 245,267 + MMJJ_RS01270 0.82 -0.8 245,317 + MMJJ_RS01270 0.90 -1.5 245,467 - +1.1 245,472 + -1.1 245,472 + +1.0 245,477 - +0.8 245,550 + MMJJ_RS01275 0.18 +1.2 245,630 - MMJJ_RS01275 0.30 +0.2 245,630 - MMJJ_RS01275 0.30 +0.5 245,722 - MMJJ_RS01275 0.43 +0.0 245,727 - MMJJ_RS01275 0.43 +0.5 245,727 - MMJJ_RS01275 0.43 -1.0 245,728 + MMJJ_RS01275 0.44 -0.7
Or see this region's nucleotide sequence