Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00430 and MMJJ_RS00435 are separated by 14 nucleotides MMJJ_RS00435 and MMJJ_RS00440 are separated by 80 nucleotides MMJJ_RS00440 and MMJJ_RS09330 are separated by 35 nucleotides MMJJ_RS09330 and MMJJ_RS00445 are separated by 87 nucleotides MMJJ_RS00445 and MMJJ_RS00450 are separated by 11 nucleotides MMJJ_RS00450 and MMJJ_RS00455 are separated by 66 nucleotides
MMJJ_RS00430: MMJJ_RS00430 - ribonuclease Z, at 77,945 to 78,871
_RS00430
MMJJ_RS00435: MMJJ_RS00435 - hypothetical protein, at 78,886 to 79,464
_RS00435
MMJJ_RS00440: MMJJ_RS00440 - selenide, water dikinase SelD, at 79,545 to 80,552
_RS00440
MMJJ_RS09330: MMJJ_RS09330 - hypothetical protein, at 80,588 to 80,746
_RS09330
MMJJ_RS00445: MMJJ_RS00445 - class III signal peptide-containing protein, at 80,834 to 81,061
_RS00445
MMJJ_RS00450: MMJJ_RS00450 - DUF126 domain-containing protein, at 81,073 to 81,465
_RS00450
MMJJ_RS00455: MMJJ_RS00455 - NOG1 family protein, at 81,532 to 82,569
_RS00455
Position (kb) 79
80
81 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 78.742 kb on + strand, within MMJJ_RS00430 at 78.775 kb on + strand, within MMJJ_RS00430 at 78.876 kb on + strand at 78.876 kb on + strand at 78.876 kb on + strand at 78.895 kb on - strand at 78.904 kb on - strand at 78.905 kb on + strand at 78.935 kb on - strand at 78.940 kb on - strand at 78.941 kb on + strand at 78.948 kb on + strand, within MMJJ_RS00435 at 79.014 kb on + strand, within MMJJ_RS00435 at 79.023 kb on - strand, within MMJJ_RS00435 at 79.028 kb on - strand, within MMJJ_RS00435 at 79.029 kb on + strand, within MMJJ_RS00435 at 79.064 kb on - strand, within MMJJ_RS00435 at 79.064 kb on - strand, within MMJJ_RS00435 at 79.069 kb on - strand, within MMJJ_RS00435 at 79.070 kb on + strand, within MMJJ_RS00435 at 79.271 kb on - strand, within MMJJ_RS00435 at 79.271 kb on - strand, within MMJJ_RS00435 at 79.271 kb on - strand, within MMJJ_RS00435 at 79.271 kb on - strand, within MMJJ_RS00435 at 79.271 kb on - strand, within MMJJ_RS00435 at 79.271 kb on - strand, within MMJJ_RS00435 at 79.272 kb on + strand, within MMJJ_RS00435 at 79.272 kb on + strand, within MMJJ_RS00435 at 79.272 kb on + strand, within MMJJ_RS00435 at 79.272 kb on + strand, within MMJJ_RS00435 at 79.272 kb on + strand, within MMJJ_RS00435 at 79.272 kb on + strand, within MMJJ_RS00435 at 79.283 kb on - strand, within MMJJ_RS00435 at 79.284 kb on + strand, within MMJJ_RS00435 at 79.332 kb on - strand, within MMJJ_RS00435 at 79.350 kb on - strand, within MMJJ_RS00435 at 79.351 kb on + strand, within MMJJ_RS00435 at 79.376 kb on - strand, within MMJJ_RS00435 at 79.377 kb on + strand, within MMJJ_RS00435 at 79.392 kb on - strand, within MMJJ_RS00435 at 79.392 kb on - strand, within MMJJ_RS00435 at 79.392 kb on - strand, within MMJJ_RS00435 at 79.398 kb on - strand, within MMJJ_RS00435 at 79.398 kb on - strand, within MMJJ_RS00435 at 79.419 kb on + strand at 79.428 kb on + strand at 79.428 kb on + strand at 79.428 kb on + strand at 79.428 kb on + strand at 79.431 kb on + strand at 79.431 kb on + strand at 79.431 kb on + strand at 79.462 kb on - strand at 79.463 kb on + strand at 79.479 kb on - strand at 79.480 kb on + strand at 79.480 kb on + strand at 79.480 kb on + strand at 79.481 kb on - strand at 79.482 kb on + strand at 79.482 kb on + strand at 79.482 kb on + strand at 79.482 kb on + strand at 79.499 kb on + strand at 79.531 kb on - strand at 79.532 kb on + strand at 79.551 kb on + strand at 79.555 kb on - strand at 79.601 kb on + strand at 79.604 kb on + strand at 79.604 kb on + strand at 79.635 kb on - strand at 79.726 kb on - strand, within MMJJ_RS00440 at 79.727 kb on + strand, within MMJJ_RS00440 at 79.764 kb on + strand, within MMJJ_RS00440 at 79.780 kb on - strand, within MMJJ_RS00440 at 79.780 kb on - strand, within MMJJ_RS00440 at 79.780 kb on - strand, within MMJJ_RS00440 at 79.780 kb on - strand, within MMJJ_RS00440 at 79.780 kb on - strand, within MMJJ_RS00440 at 79.780 kb on - strand, within MMJJ_RS00440 at 79.780 kb on - strand, within MMJJ_RS00440 at 79.781 kb on + strand, within MMJJ_RS00440 at 79.781 kb on + strand, within MMJJ_RS00440 at 79.781 kb on + strand, within MMJJ_RS00440 at 79.781 kb on + strand, within MMJJ_RS00440 at 79.783 kb on + strand, within MMJJ_RS00440 at 79.819 kb on - strand, within MMJJ_RS00440 at 79.933 kb on - strand, within MMJJ_RS00440 at 79.949 kb on + strand, within MMJJ_RS00440 at 79.949 kb on + strand, within MMJJ_RS00440 at 79.995 kb on + strand, within MMJJ_RS00440 at 79.995 kb on + strand, within MMJJ_RS00440 at 79.995 kb on + strand, within MMJJ_RS00440 at 79.995 kb on + strand, within MMJJ_RS00440 at 80.009 kb on - strand, within MMJJ_RS00440 at 80.024 kb on + strand, within MMJJ_RS00440 at 80.169 kb on + strand, within MMJJ_RS00440 at 80.407 kb on - strand, within MMJJ_RS00440 at 80.590 kb on + strand at 80.610 kb on - strand, within MMJJ_RS09330 at 80.683 kb on - strand, within MMJJ_RS09330 at 80.684 kb on + strand, within MMJJ_RS09330 at 80.684 kb on + strand, within MMJJ_RS09330 at 80.708 kb on - strand, within MMJJ_RS09330 at 80.708 kb on - strand, within MMJJ_RS09330 at 80.708 kb on - strand, within MMJJ_RS09330 at 80.709 kb on + strand, within MMJJ_RS09330 at 80.709 kb on + strand, within MMJJ_RS09330 at 80.709 kb on + strand, within MMJJ_RS09330 at 80.718 kb on - strand, within MMJJ_RS09330 at 80.719 kb on + strand, within MMJJ_RS09330 at 80.750 kb on + strand at 80.750 kb on + strand at 80.768 kb on - strand at 80.768 kb on - strand at 80.772 kb on - strand at 80.773 kb on + strand at 80.773 kb on + strand at 80.773 kb on + strand at 80.774 kb on - strand at 80.784 kb on - strand at 80.794 kb on - strand at 80.794 kb on - strand at 80.795 kb on + strand at 80.799 kb on + strand at 80.805 kb on + strand at 80.816 kb on + strand at 80.823 kb on + strand at 80.912 kb on + strand, within MMJJ_RS00445 at 80.923 kb on + strand, within MMJJ_RS00445 at 80.923 kb on + strand, within MMJJ_RS00445 at 80.923 kb on + strand, within MMJJ_RS00445 at 80.934 kb on + strand, within MMJJ_RS00445 at 80.939 kb on - strand, within MMJJ_RS00445 at 80.943 kb on - strand, within MMJJ_RS00445 at 80.944 kb on + strand, within MMJJ_RS00445 at 80.944 kb on + strand, within MMJJ_RS00445 at 80.944 kb on + strand, within MMJJ_RS00445 at 80.950 kb on - strand, within MMJJ_RS00445 at 80.968 kb on - strand at 80.974 kb on + strand, within MMJJ_RS00445 at 81.008 kb on + strand, within MMJJ_RS00445 at 81.008 kb on + strand, within MMJJ_RS00445 at 81.029 kb on + strand, within MMJJ_RS00445 at 81.048 kb on - strand at 81.049 kb on + strand at 81.049 kb on + strand at 81.231 kb on + strand, within MMJJ_RS00450 at 81.271 kb on - strand, within MMJJ_RS00450 at 81.388 kb on + strand, within MMJJ_RS00450 at 81.416 kb on - strand, within MMJJ_RS00450 at 81.417 kb on + strand at 81.505 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 78,742 + MMJJ_RS00430 0.86 -1.7 78,775 + MMJJ_RS00430 0.90 -1.3 78,876 + -2.0 78,876 + +1.1 78,876 + +0.1 78,895 - -0.1 78,904 - -0.7 78,905 + +0.6 78,935 - -2.2 78,940 - +0.3 78,941 + +0.6 78,948 + MMJJ_RS00435 0.11 +1.2 79,014 + MMJJ_RS00435 0.22 -1.3 79,023 - MMJJ_RS00435 0.24 -0.6 79,028 - MMJJ_RS00435 0.25 -0.1 79,029 + MMJJ_RS00435 0.25 +0.8 79,064 - MMJJ_RS00435 0.31 -0.7 79,064 - MMJJ_RS00435 0.31 +0.2 79,069 - MMJJ_RS00435 0.32 +0.6 79,070 + MMJJ_RS00435 0.32 +1.3 79,271 - MMJJ_RS00435 0.66 -0.3 79,271 - MMJJ_RS00435 0.66 +0.4 79,271 - MMJJ_RS00435 0.66 -0.7 79,271 - MMJJ_RS00435 0.66 -0.4 79,271 - MMJJ_RS00435 0.66 +0.7 79,271 - MMJJ_RS00435 0.66 -0.3 79,272 + MMJJ_RS00435 0.67 -0.1 79,272 + MMJJ_RS00435 0.67 -1.2 79,272 + MMJJ_RS00435 0.67 +2.0 79,272 + MMJJ_RS00435 0.67 +1.9 79,272 + MMJJ_RS00435 0.67 -1.1 79,272 + MMJJ_RS00435 0.67 -0.9 79,283 - MMJJ_RS00435 0.69 -0.1 79,284 + MMJJ_RS00435 0.69 +1.1 79,332 - MMJJ_RS00435 0.77 +0.2 79,350 - MMJJ_RS00435 0.80 -0.4 79,351 + MMJJ_RS00435 0.80 +1.9 79,376 - MMJJ_RS00435 0.85 -0.1 79,377 + MMJJ_RS00435 0.85 +0.1 79,392 - MMJJ_RS00435 0.87 -0.5 79,392 - MMJJ_RS00435 0.87 -0.6 79,392 - MMJJ_RS00435 0.87 +1.6 79,398 - MMJJ_RS00435 0.88 -1.0 79,398 - MMJJ_RS00435 0.88 +0.8 79,419 + +0.2 79,428 + +0.8 79,428 + -0.5 79,428 + +0.9 79,428 + -1.7 79,431 + +1.3 79,431 + -1.5 79,431 + -0.6 79,462 - +1.2 79,463 + +0.4 79,479 - +0.2 79,480 + +0.9 79,480 + +0.0 79,480 + +0.1 79,481 - +0.3 79,482 + -0.3 79,482 + -0.2 79,482 + -0.4 79,482 + -0.1 79,499 + +0.0 79,531 - +1.8 79,532 + -0.8 79,551 + +0.4 79,555 - +2.2 79,601 + +0.6 79,604 + +1.6 79,604 + -0.5 79,635 - -1.2 79,726 - MMJJ_RS00440 0.18 +1.6 79,727 + MMJJ_RS00440 0.18 +0.4 79,764 + MMJJ_RS00440 0.22 +0.9 79,780 - MMJJ_RS00440 0.23 +1.4 79,780 - MMJJ_RS00440 0.23 +0.3 79,780 - MMJJ_RS00440 0.23 +0.7 79,780 - MMJJ_RS00440 0.23 +0.4 79,780 - MMJJ_RS00440 0.23 +2.0 79,780 - MMJJ_RS00440 0.23 +0.1 79,780 - MMJJ_RS00440 0.23 -0.4 79,781 + MMJJ_RS00440 0.23 +0.3 79,781 + MMJJ_RS00440 0.23 +1.0 79,781 + MMJJ_RS00440 0.23 +1.1 79,781 + MMJJ_RS00440 0.23 -0.5 79,783 + MMJJ_RS00440 0.24 +0.1 79,819 - MMJJ_RS00440 0.27 -0.2 79,933 - MMJJ_RS00440 0.38 +0.8 79,949 + MMJJ_RS00440 0.40 +0.4 79,949 + MMJJ_RS00440 0.40 +1.1 79,995 + MMJJ_RS00440 0.45 +2.4 79,995 + MMJJ_RS00440 0.45 +0.6 79,995 + MMJJ_RS00440 0.45 +1.0 79,995 + MMJJ_RS00440 0.45 -0.0 80,009 - MMJJ_RS00440 0.46 +0.2 80,024 + MMJJ_RS00440 0.48 +1.9 80,169 + MMJJ_RS00440 0.62 +0.0 80,407 - MMJJ_RS00440 0.86 +1.0 80,590 + +1.3 80,610 - MMJJ_RS09330 0.14 +1.2 80,683 - MMJJ_RS09330 0.60 -1.5 80,684 + MMJJ_RS09330 0.60 -0.3 80,684 + MMJJ_RS09330 0.60 +0.3 80,708 - MMJJ_RS09330 0.75 -0.7 80,708 - MMJJ_RS09330 0.75 -0.5 80,708 - MMJJ_RS09330 0.75 -0.0 80,709 + MMJJ_RS09330 0.76 +0.8 80,709 + MMJJ_RS09330 0.76 -0.2 80,709 + MMJJ_RS09330 0.76 +0.3 80,718 - MMJJ_RS09330 0.82 +0.9 80,719 + MMJJ_RS09330 0.82 -1.4 80,750 + -1.4 80,750 + -1.0 80,768 - +0.7 80,768 - +0.4 80,772 - +1.4 80,773 + -1.1 80,773 + -1.2 80,773 + +1.3 80,774 - +1.1 80,784 - -0.3 80,794 - -0.5 80,794 - -2.3 80,795 + +0.9 80,799 + -1.5 80,805 + -0.4 80,816 + -1.4 80,823 + -0.4 80,912 + MMJJ_RS00445 0.34 +0.3 80,923 + MMJJ_RS00445 0.39 +1.5 80,923 + MMJJ_RS00445 0.39 -0.1 80,923 + MMJJ_RS00445 0.39 +0.5 80,934 + MMJJ_RS00445 0.44 -0.5 80,939 - MMJJ_RS00445 0.46 -0.5 80,943 - MMJJ_RS00445 0.48 -2.3 80,944 + MMJJ_RS00445 0.48 -0.7 80,944 + MMJJ_RS00445 0.48 +0.4 80,944 + MMJJ_RS00445 0.48 +1.5 80,950 - MMJJ_RS00445 0.51 -2.3 80,968 - -0.3 80,974 + MMJJ_RS00445 0.61 -2.9 81,008 + MMJJ_RS00445 0.76 +2.2 81,008 + MMJJ_RS00445 0.76 +0.5 81,029 + MMJJ_RS00445 0.86 +0.9 81,048 - -1.0 81,049 + -1.4 81,049 + -1.7 81,231 + MMJJ_RS00450 0.40 -2.4 81,271 - MMJJ_RS00450 0.50 -1.7 81,388 + MMJJ_RS00450 0.80 -2.5 81,416 - MMJJ_RS00450 0.87 +1.3 81,417 + +1.3 81,505 + -2.2
Or see this region's nucleotide sequence