Experiment: R2A pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04310 and LRK54_RS04315 are separated by 108 nucleotides LRK54_RS04315 and LRK54_RS04320 are separated by 5 nucleotides
LRK54_RS04310: LRK54_RS04310 - 3-dehydroquinate synthase, at 910,560 to 911,651
_RS04310
LRK54_RS04315: LRK54_RS04315 - WGR domain-containing protein, at 911,760 to 912,020
_RS04315
LRK54_RS04320: LRK54_RS04320 - uroporphyrinogen decarboxylase, at 912,026 to 913,096
_RS04320
Position (kb)
911
912
913 Strain fitness (log2 ratio)
-2
-1
0
1 at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.624 kb on - strand at 911.708 kb on + strand at 911.708 kb on + strand at 911.708 kb on + strand at 911.708 kb on + strand at 911.709 kb on - strand at 911.709 kb on - strand at 911.709 kb on - strand at 911.710 kb on + strand at 911.710 kb on + strand at 911.711 kb on - strand at 911.711 kb on - strand at 911.711 kb on - strand at 911.769 kb on + strand at 911.769 kb on + strand at 911.769 kb on + strand at 911.808 kb on + strand, within LRK54_RS04315 at 911.808 kb on + strand, within LRK54_RS04315 at 911.856 kb on + strand, within LRK54_RS04315 at 912.009 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A pH7 remove 911,623 + +0.4 911,623 + -0.4 911,623 + -1.3 911,623 + -0.4 911,623 + -1.2 911,623 + -0.1 911,623 + +0.1 911,624 - +0.4 911,708 + -1.8 911,708 + +1.4 911,708 + -0.1 911,708 + -1.8 911,709 - -0.6 911,709 - +0.1 911,709 - +0.1 911,710 + -0.7 911,710 + -0.2 911,711 - +1.1 911,711 - -0.6 911,711 - -0.1 911,769 + -0.2 911,769 + +1.1 911,769 + -0.2 911,808 + LRK54_RS04315 0.18 +1.4 911,808 + LRK54_RS04315 0.18 -1.1 911,856 + LRK54_RS04315 0.37 -0.3 912,009 + -0.1
Or see this region's nucleotide sequence