Experiment: R2A pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04090 and LRK54_RS04095 are separated by 98 nucleotides LRK54_RS04095 and LRK54_RS04100 overlap by 2 nucleotides LRK54_RS04100 and LRK54_RS04105 overlap by 4 nucleotides
LRK54_RS04090: LRK54_RS04090 - response regulator transcription factor, at 863,902 to 864,633
_RS04090
LRK54_RS04095: LRK54_RS04095 - arylesterase, at 864,732 to 865,343
_RS04095
LRK54_RS04100: LRK54_RS04100 - ATP-binding cassette domain-containing protein, at 865,342 to 866,043
_RS04100
LRK54_RS04105: LRK54_RS04105 - FtsX-like permease family protein, at 866,040 to 868,523
_RS04105
Position (kb) 865
866
867 Strain fitness (log2 ratio) -2
-1
0
1
2 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.476 kb on + strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.477 kb on - strand, within LRK54_RS04090 at 864.524 kb on + strand, within LRK54_RS04090 at 864.524 kb on + strand, within LRK54_RS04090 at 864.524 kb on + strand, within LRK54_RS04090 at 864.525 kb on - strand, within LRK54_RS04090 at 864.525 kb on - strand, within LRK54_RS04090 at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.625 kb on + strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.626 kb on - strand at 864.627 kb on - strand at 864.640 kb on + strand at 864.640 kb on + strand at 864.640 kb on + strand at 864.640 kb on + strand at 864.640 kb on + strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.641 kb on - strand at 864.658 kb on + strand at 864.658 kb on + strand at 864.658 kb on + strand at 864.658 kb on + strand at 864.658 kb on + strand at 864.658 kb on + strand at 864.659 kb on - strand at 864.659 kb on - strand at 864.659 kb on - strand at 864.659 kb on - strand at 864.659 kb on - strand at 864.670 kb on + strand at 864.671 kb on - strand at 864.898 kb on + strand, within LRK54_RS04095 at 864.898 kb on + strand, within LRK54_RS04095 at 864.898 kb on + strand, within LRK54_RS04095 at 864.898 kb on + strand, within LRK54_RS04095 at 864.898 kb on + strand, within LRK54_RS04095 at 864.898 kb on + strand, within LRK54_RS04095 at 864.899 kb on - strand, within LRK54_RS04095 at 864.899 kb on - strand, within LRK54_RS04095 at 864.899 kb on - strand, within LRK54_RS04095 at 864.899 kb on - strand, within LRK54_RS04095 at 864.899 kb on - strand, within LRK54_RS04095 at 864.899 kb on - strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.934 kb on + strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 864.935 kb on - strand, within LRK54_RS04095 at 865.090 kb on + strand, within LRK54_RS04095 at 865.090 kb on + strand, within LRK54_RS04095 at 865.090 kb on + strand, within LRK54_RS04095 at 865.090 kb on + strand, within LRK54_RS04095 at 865.090 kb on + strand, within LRK54_RS04095 at 865.090 kb on + strand, within LRK54_RS04095 at 865.091 kb on - strand, within LRK54_RS04095 at 865.091 kb on - strand, within LRK54_RS04095 at 865.091 kb on - strand, within LRK54_RS04095 at 865.177 kb on - strand, within LRK54_RS04095 at 865.383 kb on + strand at 865.383 kb on + strand at 865.383 kb on + strand at 865.383 kb on + strand at 865.383 kb on + strand at 865.383 kb on + strand at 865.383 kb on + strand at 865.383 kb on + strand at 865.383 kb on + strand at 865.383 kb on + strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.384 kb on - strand at 865.769 kb on - strand, within LRK54_RS04100 at 865.769 kb on - strand, within LRK54_RS04100 at 865.769 kb on - strand, within LRK54_RS04100 at 866.199 kb on + strand at 866.224 kb on + strand at 866.224 kb on + strand at 866.224 kb on + strand at 866.224 kb on + strand at 866.225 kb on - strand at 866.225 kb on - strand at 866.251 kb on + strand at 866.251 kb on + strand at 866.252 kb on - strand at 866.371 kb on - strand, within LRK54_RS04105 at 866.371 kb on - strand, within LRK54_RS04105 at 866.371 kb on - strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.451 kb on + strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.452 kb on - strand, within LRK54_RS04105 at 866.868 kb on + strand, within LRK54_RS04105 at 866.868 kb on + strand, within LRK54_RS04105 at 866.869 kb on - strand, within LRK54_RS04105 at 866.869 kb on - strand, within LRK54_RS04105 at 866.952 kb on - strand, within LRK54_RS04105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A pH7 remove 864,476 + LRK54_RS04090 0.78 +0.3 864,476 + LRK54_RS04090 0.78 -0.4 864,476 + LRK54_RS04090 0.78 +0.2 864,476 + LRK54_RS04090 0.78 +0.2 864,476 + LRK54_RS04090 0.78 -0.9 864,476 + LRK54_RS04090 0.78 -0.3 864,476 + LRK54_RS04090 0.78 +0.3 864,476 + LRK54_RS04090 0.78 -0.2 864,476 + LRK54_RS04090 0.78 -1.3 864,476 + LRK54_RS04090 0.78 -0.8 864,476 + LRK54_RS04090 0.78 +0.3 864,476 + LRK54_RS04090 0.78 -0.0 864,476 + LRK54_RS04090 0.78 +0.5 864,476 + LRK54_RS04090 0.78 -1.0 864,476 + LRK54_RS04090 0.78 -0.1 864,476 + LRK54_RS04090 0.78 +0.3 864,476 + LRK54_RS04090 0.78 -0.2 864,476 + LRK54_RS04090 0.78 -0.2 864,476 + LRK54_RS04090 0.78 +0.1 864,476 + LRK54_RS04090 0.78 -0.5 864,476 + LRK54_RS04090 0.78 +2.4 864,476 + LRK54_RS04090 0.78 +0.8 864,476 + LRK54_RS04090 0.78 +2.4 864,476 + LRK54_RS04090 0.78 -0.3 864,476 + LRK54_RS04090 0.78 -1.6 864,476 + LRK54_RS04090 0.78 -1.2 864,476 + LRK54_RS04090 0.78 -0.8 864,476 + LRK54_RS04090 0.78 -0.4 864,476 + LRK54_RS04090 0.78 +0.6 864,476 + LRK54_RS04090 0.78 -0.2 864,476 + LRK54_RS04090 0.78 +0.1 864,476 + LRK54_RS04090 0.78 +0.1 864,476 + LRK54_RS04090 0.78 -0.5 864,476 + LRK54_RS04090 0.78 +0.9 864,476 + LRK54_RS04090 0.78 -0.6 864,476 + LRK54_RS04090 0.78 -0.0 864,476 + LRK54_RS04090 0.78 -1.4 864,476 + LRK54_RS04090 0.78 +0.8 864,476 + LRK54_RS04090 0.78 -1.4 864,476 + LRK54_RS04090 0.78 -0.0 864,477 - LRK54_RS04090 0.79 -0.7 864,477 - LRK54_RS04090 0.79 -0.2 864,477 - LRK54_RS04090 0.79 +2.0 864,477 - LRK54_RS04090 0.79 -0.7 864,477 - LRK54_RS04090 0.79 -2.3 864,477 - LRK54_RS04090 0.79 -1.2 864,477 - LRK54_RS04090 0.79 -1.4 864,477 - LRK54_RS04090 0.79 -0.1 864,477 - LRK54_RS04090 0.79 -1.3 864,477 - LRK54_RS04090 0.79 -0.3 864,477 - LRK54_RS04090 0.79 +1.4 864,477 - LRK54_RS04090 0.79 +2.0 864,477 - LRK54_RS04090 0.79 +0.2 864,477 - LRK54_RS04090 0.79 +1.1 864,477 - LRK54_RS04090 0.79 -1.0 864,477 - LRK54_RS04090 0.79 +0.6 864,477 - LRK54_RS04090 0.79 +1.1 864,477 - LRK54_RS04090 0.79 +0.8 864,477 - LRK54_RS04090 0.79 +1.4 864,477 - LRK54_RS04090 0.79 +0.2 864,477 - LRK54_RS04090 0.79 -1.4 864,477 - LRK54_RS04090 0.79 +0.1 864,477 - LRK54_RS04090 0.79 +0.4 864,477 - LRK54_RS04090 0.79 +1.4 864,477 - LRK54_RS04090 0.79 +0.8 864,477 - LRK54_RS04090 0.79 +0.7 864,477 - LRK54_RS04090 0.79 -0.9 864,477 - LRK54_RS04090 0.79 +0.3 864,477 - LRK54_RS04090 0.79 -0.5 864,477 - LRK54_RS04090 0.79 +0.2 864,477 - LRK54_RS04090 0.79 +1.0 864,477 - LRK54_RS04090 0.79 +1.4 864,477 - LRK54_RS04090 0.79 +0.1 864,524 + LRK54_RS04090 0.85 -0.8 864,524 + LRK54_RS04090 0.85 -0.4 864,524 + LRK54_RS04090 0.85 +0.2 864,525 - LRK54_RS04090 0.85 +0.3 864,525 - LRK54_RS04090 0.85 +0.2 864,625 + +0.2 864,625 + +0.3 864,625 + -0.5 864,625 + -0.4 864,625 + -0.1 864,625 + -0.6 864,625 + -0.4 864,625 + -0.2 864,625 + -0.1 864,625 + -1.0 864,625 + +1.4 864,625 + -1.2 864,625 + +0.2 864,625 + +1.1 864,625 + -0.1 864,625 + -0.4 864,625 + -0.9 864,625 + -0.3 864,625 + -0.8 864,625 + +0.3 864,625 + -0.2 864,625 + -0.0 864,626 - +0.7 864,626 - -0.2 864,626 - +0.2 864,626 - +0.1 864,626 - +0.8 864,626 - -1.0 864,626 - +0.3 864,626 - +0.2 864,626 - +0.1 864,626 - +0.2 864,626 - -0.4 864,626 - -0.9 864,626 - -0.2 864,626 - +1.4 864,626 - -0.2 864,626 - -0.8 864,626 - +1.0 864,626 - -0.2 864,626 - -0.2 864,626 - +1.6 864,626 - -0.5 864,626 - -0.9 864,626 - +0.0 864,626 - +0.4 864,626 - -0.5 864,627 - -0.7 864,640 + +0.3 864,640 + +0.1 864,640 + +0.4 864,640 + -2.2 864,640 + -2.0 864,641 - +0.7 864,641 - +0.5 864,641 - +0.4 864,641 - +0.6 864,641 - +1.3 864,641 - -0.1 864,641 - +0.5 864,641 - +0.8 864,641 - -0.5 864,641 - +0.1 864,641 - -0.8 864,641 - -1.3 864,641 - +0.4 864,658 + -2.4 864,658 + +0.9 864,658 + -0.6 864,658 + +0.1 864,658 + +0.2 864,658 + +1.4 864,659 - -0.0 864,659 - +0.0 864,659 - -0.1 864,659 - +0.2 864,659 - +0.4 864,670 + -0.9 864,671 - +2.2 864,898 + LRK54_RS04095 0.27 +0.1 864,898 + LRK54_RS04095 0.27 -0.4 864,898 + LRK54_RS04095 0.27 +0.2 864,898 + LRK54_RS04095 0.27 -0.6 864,898 + LRK54_RS04095 0.27 +0.2 864,898 + LRK54_RS04095 0.27 +1.1 864,899 - LRK54_RS04095 0.27 +0.2 864,899 - LRK54_RS04095 0.27 +0.1 864,899 - LRK54_RS04095 0.27 +0.5 864,899 - LRK54_RS04095 0.27 -0.4 864,899 - LRK54_RS04095 0.27 +0.4 864,899 - LRK54_RS04095 0.27 +0.5 864,934 + LRK54_RS04095 0.33 -1.6 864,934 + LRK54_RS04095 0.33 +0.5 864,934 + LRK54_RS04095 0.33 -1.0 864,934 + LRK54_RS04095 0.33 +0.1 864,934 + LRK54_RS04095 0.33 +0.1 864,934 + LRK54_RS04095 0.33 +0.9 864,934 + LRK54_RS04095 0.33 +0.8 864,934 + LRK54_RS04095 0.33 +1.7 864,934 + LRK54_RS04095 0.33 +0.4 864,935 - LRK54_RS04095 0.33 +0.7 864,935 - LRK54_RS04095 0.33 -0.2 864,935 - LRK54_RS04095 0.33 -1.4 864,935 - LRK54_RS04095 0.33 +0.3 864,935 - LRK54_RS04095 0.33 +0.2 864,935 - LRK54_RS04095 0.33 -0.2 864,935 - LRK54_RS04095 0.33 -1.7 864,935 - LRK54_RS04095 0.33 -1.4 864,935 - LRK54_RS04095 0.33 +0.2 865,090 + LRK54_RS04095 0.58 +2.4 865,090 + LRK54_RS04095 0.58 +0.4 865,090 + LRK54_RS04095 0.58 +1.3 865,090 + LRK54_RS04095 0.58 -0.6 865,090 + LRK54_RS04095 0.58 +0.9 865,090 + LRK54_RS04095 0.58 -0.4 865,091 - LRK54_RS04095 0.59 -2.0 865,091 - LRK54_RS04095 0.59 +0.0 865,091 - LRK54_RS04095 0.59 +1.3 865,177 - LRK54_RS04095 0.73 -0.9 865,383 + -0.2 865,383 + -0.5 865,383 + -1.3 865,383 + -1.3 865,383 + -0.1 865,383 + +0.8 865,383 + +1.0 865,383 + -0.0 865,383 + +0.7 865,383 + +0.1 865,384 - +1.8 865,384 - -0.3 865,384 - -0.6 865,384 - +0.3 865,384 - -0.1 865,384 - +0.2 865,384 - -0.5 865,384 - -1.0 865,384 - +0.0 865,384 - -1.3 865,384 - +0.5 865,384 - +0.4 865,769 - LRK54_RS04100 0.61 +0.5 865,769 - LRK54_RS04100 0.61 +0.5 865,769 - LRK54_RS04100 0.61 +0.2 866,199 + -0.9 866,224 + +1.4 866,224 + +0.4 866,224 + +0.0 866,224 + -0.5 866,225 - -0.5 866,225 - -0.3 866,251 + +0.1 866,251 + +0.6 866,252 - -1.3 866,371 - LRK54_RS04105 0.13 -1.0 866,371 - LRK54_RS04105 0.13 -0.0 866,371 - LRK54_RS04105 0.13 +0.1 866,451 + LRK54_RS04105 0.17 -0.6 866,451 + LRK54_RS04105 0.17 +0.8 866,451 + LRK54_RS04105 0.17 +0.4 866,451 + LRK54_RS04105 0.17 -0.0 866,451 + LRK54_RS04105 0.17 -0.6 866,451 + LRK54_RS04105 0.17 -0.3 866,451 + LRK54_RS04105 0.17 -0.5 866,451 + LRK54_RS04105 0.17 +0.1 866,451 + LRK54_RS04105 0.17 +1.2 866,451 + LRK54_RS04105 0.17 -1.3 866,451 + LRK54_RS04105 0.17 -1.9 866,452 - LRK54_RS04105 0.17 +0.3 866,452 - LRK54_RS04105 0.17 -0.5 866,452 - LRK54_RS04105 0.17 +2.4 866,452 - LRK54_RS04105 0.17 +2.1 866,452 - LRK54_RS04105 0.17 -0.8 866,452 - LRK54_RS04105 0.17 -1.4 866,452 - LRK54_RS04105 0.17 -0.2 866,452 - LRK54_RS04105 0.17 +1.4 866,452 - LRK54_RS04105 0.17 +0.2 866,452 - LRK54_RS04105 0.17 -0.1 866,452 - LRK54_RS04105 0.17 -0.4 866,868 + LRK54_RS04105 0.33 +0.1 866,868 + LRK54_RS04105 0.33 -0.9 866,869 - LRK54_RS04105 0.33 -0.6 866,869 - LRK54_RS04105 0.33 +1.1 866,952 - LRK54_RS04105 0.37 -0.0
Or see this region's nucleotide sequence