Experiment: R2A pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS03850 and LRK54_RS03855 are separated by 124 nucleotides LRK54_RS03855 and LRK54_RS03860 are separated by 61 nucleotides LRK54_RS03860 and LRK54_RS03865 are separated by 47 nucleotides
LRK54_RS03850: LRK54_RS03850 - NAD(P) transhydrogenase subunit alpha, at 809,539 to 810,642
_RS03850
LRK54_RS03855: LRK54_RS03855 - cytidine deaminase, at 810,767 to 811,183
_RS03855
LRK54_RS03860: LRK54_RS03860 - nitroreductase, at 811,245 to 811,814
_RS03860
LRK54_RS03865: LRK54_RS03865 - exodeoxyribonuclease IX, at 811,862 to 812,716
_RS03865
Position (kb)
811
812 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 810.276 kb on + strand, within LRK54_RS03850 at 810.353 kb on + strand, within LRK54_RS03850 at 810.354 kb on - strand, within LRK54_RS03850 at 810.382 kb on - strand, within LRK54_RS03850 at 810.382 kb on - strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.538 kb on + strand at 810.538 kb on + strand at 810.538 kb on + strand at 810.539 kb on - strand at 810.539 kb on - strand at 810.539 kb on - strand at 810.539 kb on - strand at 810.629 kb on - strand at 810.675 kb on + strand at 810.675 kb on + strand at 810.675 kb on + strand at 810.675 kb on + strand at 810.676 kb on - strand at 810.676 kb on - strand at 810.676 kb on - strand at 810.676 kb on - strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.718 kb on - strand at 810.718 kb on - strand at 810.718 kb on - strand at 810.718 kb on - strand at 810.718 kb on - strand at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.842 kb on + strand, within LRK54_RS03855 at 810.842 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 811.211 kb on + strand at 811.212 kb on - strand at 811.420 kb on + strand, within LRK54_RS03860 at 811.420 kb on + strand, within LRK54_RS03860 at 811.420 kb on + strand, within LRK54_RS03860 at 811.420 kb on + strand, within LRK54_RS03860 at 811.421 kb on - strand, within LRK54_RS03860 at 811.421 kb on - strand, within LRK54_RS03860 at 811.506 kb on + strand, within LRK54_RS03860 at 811.506 kb on + strand, within LRK54_RS03860 at 811.506 kb on + strand, within LRK54_RS03860 at 811.507 kb on - strand, within LRK54_RS03860 at 811.507 kb on - strand, within LRK54_RS03860 at 811.507 kb on - strand, within LRK54_RS03860 at 811.626 kb on + strand, within LRK54_RS03860 at 811.626 kb on + strand, within LRK54_RS03860 at 811.626 kb on + strand, within LRK54_RS03860 at 811.626 kb on + strand, within LRK54_RS03860 at 811.627 kb on - strand, within LRK54_RS03860 at 811.627 kb on - strand, within LRK54_RS03860 at 811.671 kb on + strand, within LRK54_RS03860 at 811.671 kb on + strand, within LRK54_RS03860 at 811.760 kb on - strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.943 kb on + strand at 811.943 kb on + strand at 812.042 kb on + strand, within LRK54_RS03865 at 812.042 kb on + strand, within LRK54_RS03865 at 812.043 kb on - strand, within LRK54_RS03865 at 812.051 kb on + strand, within LRK54_RS03865 at 812.051 kb on + strand, within LRK54_RS03865 at 812.051 kb on + strand, within LRK54_RS03865 at 812.051 kb on + strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.282 kb on + strand, within LRK54_RS03865 at 812.282 kb on + strand, within LRK54_RS03865 at 812.283 kb on - strand, within LRK54_RS03865 at 812.283 kb on - strand, within LRK54_RS03865 at 812.283 kb on - strand, within LRK54_RS03865 at 812.357 kb on + strand, within LRK54_RS03865 at 812.357 kb on + strand, within LRK54_RS03865 at 812.357 kb on + strand, within LRK54_RS03865 at 812.358 kb on - strand, within LRK54_RS03865 at 812.358 kb on - strand, within LRK54_RS03865 at 812.358 kb on - strand, within LRK54_RS03865 at 812.367 kb on + strand, within LRK54_RS03865 at 812.367 kb on + strand, within LRK54_RS03865 at 812.368 kb on - strand, within LRK54_RS03865 at 812.368 kb on - strand, within LRK54_RS03865 at 812.368 kb on - strand, within LRK54_RS03865
Per-strain Table
Position Strand Gene LocusTag Fraction R2A pH7 remove 810,276 + LRK54_RS03850 0.67 +0.3 810,353 + LRK54_RS03850 0.74 -0.4 810,354 - LRK54_RS03850 0.74 -0.5 810,382 - LRK54_RS03850 0.76 -0.1 810,382 - LRK54_RS03850 0.76 -1.5 810,436 + LRK54_RS03850 0.81 -0.2 810,436 + LRK54_RS03850 0.81 +0.1 810,436 + LRK54_RS03850 0.81 -0.9 810,436 + LRK54_RS03850 0.81 -0.2 810,436 + LRK54_RS03850 0.81 +1.4 810,436 + LRK54_RS03850 0.81 -0.1 810,436 + LRK54_RS03850 0.81 -1.5 810,436 + LRK54_RS03850 0.81 +1.8 810,437 - LRK54_RS03850 0.81 -0.4 810,437 - LRK54_RS03850 0.81 -1.7 810,437 - LRK54_RS03850 0.81 +0.5 810,437 - LRK54_RS03850 0.81 -1.3 810,437 - LRK54_RS03850 0.81 -0.4 810,437 - LRK54_RS03850 0.81 -0.0 810,488 + LRK54_RS03850 0.86 -0.0 810,488 + LRK54_RS03850 0.86 -0.9 810,488 + LRK54_RS03850 0.86 -0.0 810,488 + LRK54_RS03850 0.86 -2.3 810,488 + LRK54_RS03850 0.86 -0.6 810,488 + LRK54_RS03850 0.86 +0.5 810,488 + LRK54_RS03850 0.86 -0.5 810,488 + LRK54_RS03850 0.86 -0.1 810,488 + LRK54_RS03850 0.86 +0.7 810,488 + LRK54_RS03850 0.86 -1.7 810,489 - LRK54_RS03850 0.86 +0.5 810,489 - LRK54_RS03850 0.86 +0.1 810,489 - LRK54_RS03850 0.86 -1.3 810,489 - LRK54_RS03850 0.86 -0.4 810,489 - LRK54_RS03850 0.86 -0.7 810,538 + -0.7 810,538 + +0.7 810,538 + -0.2 810,539 - -0.1 810,539 - -0.2 810,539 - -0.1 810,539 - +0.1 810,629 - -1.7 810,675 + +0.1 810,675 + -0.1 810,675 + +0.4 810,675 + +0.3 810,676 - -0.2 810,676 - -0.2 810,676 - +0.8 810,676 - -1.9 810,717 + -0.4 810,717 + -0.8 810,717 + +0.0 810,717 + -1.0 810,717 + -1.0 810,717 + -1.0 810,717 + -1.1 810,717 + -1.2 810,717 + -1.5 810,718 - -0.7 810,718 - -1.2 810,718 - -1.5 810,718 - +0.2 810,718 - -1.7 810,833 + LRK54_RS03855 0.16 +0.5 810,833 + LRK54_RS03855 0.16 +0.9 810,833 + LRK54_RS03855 0.16 -0.2 810,833 + LRK54_RS03855 0.16 +1.7 810,833 + LRK54_RS03855 0.16 -1.1 810,833 + LRK54_RS03855 0.16 +0.3 810,833 + LRK54_RS03855 0.16 +1.3 810,833 + LRK54_RS03855 0.16 -0.3 810,833 + LRK54_RS03855 0.16 -0.3 810,833 + LRK54_RS03855 0.16 +0.3 810,833 + LRK54_RS03855 0.16 +0.1 810,834 - LRK54_RS03855 0.16 -0.6 810,834 - LRK54_RS03855 0.16 -0.3 810,834 - LRK54_RS03855 0.16 -0.5 810,834 - LRK54_RS03855 0.16 +0.1 810,834 - LRK54_RS03855 0.16 -0.3 810,834 - LRK54_RS03855 0.16 -0.3 810,834 - LRK54_RS03855 0.16 -0.7 810,834 - LRK54_RS03855 0.16 -0.4 810,834 - LRK54_RS03855 0.16 +0.3 810,834 - LRK54_RS03855 0.16 -1.0 810,834 - LRK54_RS03855 0.16 +2.2 810,834 - LRK54_RS03855 0.16 -0.8 810,842 + LRK54_RS03855 0.18 -0.1 810,842 + LRK54_RS03855 0.18 +0.3 810,923 + LRK54_RS03855 0.37 -1.8 810,923 + LRK54_RS03855 0.37 +0.3 810,923 + LRK54_RS03855 0.37 -1.3 810,923 + LRK54_RS03855 0.37 +0.4 810,923 + LRK54_RS03855 0.37 -0.2 810,923 + LRK54_RS03855 0.37 -0.0 810,923 + LRK54_RS03855 0.37 +0.1 810,923 + LRK54_RS03855 0.37 -1.0 810,923 + LRK54_RS03855 0.37 -0.7 810,923 + LRK54_RS03855 0.37 -0.2 810,923 + LRK54_RS03855 0.37 +0.4 810,923 + LRK54_RS03855 0.37 -0.4 810,924 - LRK54_RS03855 0.38 +0.4 810,924 - LRK54_RS03855 0.38 -0.3 810,924 - LRK54_RS03855 0.38 +0.4 810,924 - LRK54_RS03855 0.38 -0.2 810,924 - LRK54_RS03855 0.38 +0.8 810,924 - LRK54_RS03855 0.38 +0.2 811,211 + +0.9 811,212 - -1.2 811,420 + LRK54_RS03860 0.31 -0.6 811,420 + LRK54_RS03860 0.31 -0.5 811,420 + LRK54_RS03860 0.31 -0.2 811,420 + LRK54_RS03860 0.31 -0.1 811,421 - LRK54_RS03860 0.31 +0.0 811,421 - LRK54_RS03860 0.31 +0.1 811,506 + LRK54_RS03860 0.46 +1.4 811,506 + LRK54_RS03860 0.46 +0.3 811,506 + LRK54_RS03860 0.46 +1.3 811,507 - LRK54_RS03860 0.46 +0.3 811,507 - LRK54_RS03860 0.46 +0.4 811,507 - LRK54_RS03860 0.46 -0.0 811,626 + LRK54_RS03860 0.67 -0.6 811,626 + LRK54_RS03860 0.67 +0.5 811,626 + LRK54_RS03860 0.67 +0.1 811,626 + LRK54_RS03860 0.67 +1.1 811,627 - LRK54_RS03860 0.67 -0.9 811,627 - LRK54_RS03860 0.67 +0.8 811,671 + LRK54_RS03860 0.75 -0.5 811,671 + LRK54_RS03860 0.75 +1.9 811,760 - +0.6 811,865 + +0.4 811,865 + -1.2 811,865 + -0.4 811,865 + -0.7 811,865 + +0.4 811,865 + -0.7 811,865 + +1.0 811,865 + -0.1 811,865 + -0.7 811,865 + -0.6 811,866 - +0.1 811,866 - -0.1 811,866 - -0.6 811,866 - -0.3 811,866 - +0.0 811,866 - -0.7 811,866 - -0.1 811,866 - -0.1 811,943 + +0.2 811,943 + +0.4 812,042 + LRK54_RS03865 0.21 -0.5 812,042 + LRK54_RS03865 0.21 -1.2 812,043 - LRK54_RS03865 0.21 +0.1 812,051 + LRK54_RS03865 0.22 -0.7 812,051 + LRK54_RS03865 0.22 +1.9 812,051 + LRK54_RS03865 0.22 -0.4 812,051 + LRK54_RS03865 0.22 +0.1 812,052 - LRK54_RS03865 0.22 +1.1 812,052 - LRK54_RS03865 0.22 +0.2 812,052 - LRK54_RS03865 0.22 +0.8 812,052 - LRK54_RS03865 0.22 -0.5 812,052 - LRK54_RS03865 0.22 -0.9 812,052 - LRK54_RS03865 0.22 -0.1 812,052 - LRK54_RS03865 0.22 +0.1 812,282 + LRK54_RS03865 0.49 +1.4 812,282 + LRK54_RS03865 0.49 -1.6 812,283 - LRK54_RS03865 0.49 -1.0 812,283 - LRK54_RS03865 0.49 +0.0 812,283 - LRK54_RS03865 0.49 +0.5 812,357 + LRK54_RS03865 0.58 +1.0 812,357 + LRK54_RS03865 0.58 +0.9 812,357 + LRK54_RS03865 0.58 +0.1 812,358 - LRK54_RS03865 0.58 +0.2 812,358 - LRK54_RS03865 0.58 +1.2 812,358 - LRK54_RS03865 0.58 +0.3 812,367 + LRK54_RS03865 0.59 +1.0 812,367 + LRK54_RS03865 0.59 +0.7 812,368 - LRK54_RS03865 0.59 -1.0 812,368 - LRK54_RS03865 0.59 -0.9 812,368 - LRK54_RS03865 0.59 -1.2
Or see this region's nucleotide sequence