Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02230 and BPHYT_RS02235 overlap by 8 nucleotides BPHYT_RS02235 and BPHYT_RS02240 are separated by 94 nucleotides BPHYT_RS02240 and BPHYT_RS02245 are separated by 85 nucleotides BPHYT_RS02245 and BPHYT_RS02250 are separated by 187 nucleotides
BPHYT_RS02230: BPHYT_RS02230 - acetyl-CoA acetyltransferase, at 512,101 to 513,294
_RS02230
BPHYT_RS02235: BPHYT_RS02235 - beta carbonic anhydrase, at 513,287 to 514,063
_RS02235
BPHYT_RS02240: BPHYT_RS02240 - isocitrate dehydrogenase kinase/phosphatase, at 514,158 to 515,996
_RS02240
BPHYT_RS02245: BPHYT_RS02245 - MerR family transcriptional regulator, at 516,082 to 516,486
_RS02245
BPHYT_RS02250: BPHYT_RS02250 - Zn-dependent hydrolase, at 516,674 to 517,747
_RS02250
Position (kb) 514
515
516 Strain fitness (log2 ratio) -1
0
1 at 513.424 kb on - strand, within BPHYT_RS02235 at 513.496 kb on - strand, within BPHYT_RS02235 at 513.554 kb on - strand, within BPHYT_RS02235 at 513.564 kb on - strand, within BPHYT_RS02235 at 514.113 kb on + strand at 514.114 kb on - strand at 514.114 kb on - strand at 514.306 kb on - strand at 514.541 kb on - strand, within BPHYT_RS02240 at 514.541 kb on - strand, within BPHYT_RS02240 at 514.582 kb on + strand, within BPHYT_RS02240 at 514.720 kb on + strand, within BPHYT_RS02240 at 514.721 kb on - strand, within BPHYT_RS02240 at 514.721 kb on - strand, within BPHYT_RS02240 at 514.721 kb on - strand, within BPHYT_RS02240 at 514.891 kb on + strand, within BPHYT_RS02240 at 514.891 kb on + strand, within BPHYT_RS02240 at 514.892 kb on - strand, within BPHYT_RS02240 at 514.964 kb on - strand, within BPHYT_RS02240 at 515.109 kb on - strand, within BPHYT_RS02240 at 515.111 kb on - strand, within BPHYT_RS02240 at 515.338 kb on + strand, within BPHYT_RS02240 at 515.356 kb on + strand, within BPHYT_RS02240 at 515.548 kb on + strand, within BPHYT_RS02240 at 515.584 kb on + strand, within BPHYT_RS02240 at 515.584 kb on + strand, within BPHYT_RS02240 at 515.585 kb on - strand, within BPHYT_RS02240 at 515.719 kb on + strand, within BPHYT_RS02240 at 515.720 kb on - strand, within BPHYT_RS02240 at 515.816 kb on - strand at 515.823 kb on - strand at 516.042 kb on - strand at 516.263 kb on + strand, within BPHYT_RS02245 at 516.330 kb on + strand, within BPHYT_RS02245 at 516.357 kb on - strand, within BPHYT_RS02245 at 516.400 kb on + strand, within BPHYT_RS02245 at 516.473 kb on + strand at 516.473 kb on + strand at 516.591 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 513,424 - BPHYT_RS02235 0.18 +0.3 513,496 - BPHYT_RS02235 0.27 -0.3 513,554 - BPHYT_RS02235 0.34 -0.9 513,564 - BPHYT_RS02235 0.36 +0.1 514,113 + -0.6 514,114 - -0.2 514,114 - -0.2 514,306 - -0.6 514,541 - BPHYT_RS02240 0.21 -0.6 514,541 - BPHYT_RS02240 0.21 -0.4 514,582 + BPHYT_RS02240 0.23 -0.3 514,720 + BPHYT_RS02240 0.31 -1.0 514,721 - BPHYT_RS02240 0.31 -0.1 514,721 - BPHYT_RS02240 0.31 +0.2 514,721 - BPHYT_RS02240 0.31 -0.7 514,891 + BPHYT_RS02240 0.40 -0.4 514,891 + BPHYT_RS02240 0.40 -0.0 514,892 - BPHYT_RS02240 0.40 -0.5 514,964 - BPHYT_RS02240 0.44 +0.6 515,109 - BPHYT_RS02240 0.52 -0.3 515,111 - BPHYT_RS02240 0.52 +0.1 515,338 + BPHYT_RS02240 0.64 -0.3 515,356 + BPHYT_RS02240 0.65 -0.6 515,548 + BPHYT_RS02240 0.76 -0.4 515,584 + BPHYT_RS02240 0.78 -0.4 515,584 + BPHYT_RS02240 0.78 -0.9 515,585 - BPHYT_RS02240 0.78 -0.4 515,719 + BPHYT_RS02240 0.85 -0.8 515,720 - BPHYT_RS02240 0.85 +0.8 515,816 - +0.2 515,823 - -0.7 516,042 - +0.7 516,263 + BPHYT_RS02245 0.45 +0.7 516,330 + BPHYT_RS02245 0.61 -0.9 516,357 - BPHYT_RS02245 0.68 -0.9 516,400 + BPHYT_RS02245 0.79 -0.5 516,473 + +0.2 516,473 + -0.7 516,591 + -0.3
Or see this region's nucleotide sequence