Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00810 and BPHYT_RS00815 are separated by 14 nucleotides BPHYT_RS00815 and BPHYT_RS00820 overlap by 4 nucleotides BPHYT_RS00820 and BPHYT_RS00825 overlap by 4 nucleotides
BPHYT_RS00810: BPHYT_RS00810 - type IV secretion system protein VirB11, at 183,124 to 184,251
_RS00810
BPHYT_RS00815: BPHYT_RS00815 - hypothetical protein, at 184,266 to 184,676
_RS00815
BPHYT_RS00820: BPHYT_RS00820 - type IV secretory pathway protein VirD4, at 184,673 to 186,457
_RS00820
BPHYT_RS00825: BPHYT_RS00825 - DNA replication protein, at 186,454 to 188,367
_RS00825
Position (kb) 184
185
186
187 Strain fitness (log2 ratio) -2
-1
0
1 at 183.697 kb on - strand, within BPHYT_RS00810 at 183.714 kb on + strand, within BPHYT_RS00810 at 183.844 kb on + strand, within BPHYT_RS00810 at 183.845 kb on - strand, within BPHYT_RS00810 at 183.845 kb on - strand, within BPHYT_RS00810 at 183.959 kb on - strand, within BPHYT_RS00810 at 184.009 kb on + strand, within BPHYT_RS00810 at 184.010 kb on - strand, within BPHYT_RS00810 at 184.140 kb on + strand at 184.141 kb on - strand at 184.141 kb on - strand at 184.584 kb on + strand, within BPHYT_RS00815 at 184.745 kb on + strand at 184.813 kb on + strand at 184.957 kb on + strand, within BPHYT_RS00820 at 184.958 kb on - strand, within BPHYT_RS00820 at 184.958 kb on - strand, within BPHYT_RS00820 at 184.967 kb on + strand, within BPHYT_RS00820 at 185.225 kb on + strand, within BPHYT_RS00820 at 185.226 kb on - strand, within BPHYT_RS00820 at 185.630 kb on + strand, within BPHYT_RS00820 at 185.630 kb on + strand, within BPHYT_RS00820 at 185.642 kb on + strand, within BPHYT_RS00820 at 185.642 kb on + strand, within BPHYT_RS00820 at 185.697 kb on - strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.783 kb on + strand, within BPHYT_RS00820 at 185.784 kb on - strand, within BPHYT_RS00820 at 186.227 kb on + strand, within BPHYT_RS00820 at 186.466 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.907 kb on + strand, within BPHYT_RS00825 at 186.908 kb on - strand, within BPHYT_RS00825 at 187.457 kb on + strand, within BPHYT_RS00825
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 183,697 - BPHYT_RS00810 0.51 +1.1 183,714 + BPHYT_RS00810 0.52 -0.4 183,844 + BPHYT_RS00810 0.64 -0.4 183,845 - BPHYT_RS00810 0.64 +0.2 183,845 - BPHYT_RS00810 0.64 +0.3 183,959 - BPHYT_RS00810 0.74 +0.0 184,009 + BPHYT_RS00810 0.78 -1.2 184,010 - BPHYT_RS00810 0.79 -1.4 184,140 + +0.0 184,141 - -0.4 184,141 - +0.1 184,584 + BPHYT_RS00815 0.77 -0.3 184,745 + -0.2 184,813 + +0.1 184,957 + BPHYT_RS00820 0.16 -1.2 184,958 - BPHYT_RS00820 0.16 -2.2 184,958 - BPHYT_RS00820 0.16 +0.9 184,967 + BPHYT_RS00820 0.16 -1.1 185,225 + BPHYT_RS00820 0.31 -0.7 185,226 - BPHYT_RS00820 0.31 +0.9 185,630 + BPHYT_RS00820 0.54 +0.1 185,630 + BPHYT_RS00820 0.54 +0.0 185,642 + BPHYT_RS00820 0.54 -0.3 185,642 + BPHYT_RS00820 0.54 +0.1 185,697 - BPHYT_RS00820 0.57 +0.5 185,750 + BPHYT_RS00820 0.60 +0.9 185,750 + BPHYT_RS00820 0.60 +0.1 185,750 + BPHYT_RS00820 0.60 +0.7 185,750 + BPHYT_RS00820 0.60 +0.1 185,751 - BPHYT_RS00820 0.60 -0.2 185,751 - BPHYT_RS00820 0.60 +0.2 185,751 - BPHYT_RS00820 0.60 -0.2 185,783 + BPHYT_RS00820 0.62 +0.4 185,784 - BPHYT_RS00820 0.62 +0.4 186,227 + BPHYT_RS00820 0.87 -1.2 186,466 + -0.9 186,619 + +0.5 186,619 + +0.4 186,619 + +0.4 186,619 + -0.5 186,907 + BPHYT_RS00825 0.24 -0.1 186,908 - BPHYT_RS00825 0.24 -0.2 187,457 + BPHYT_RS00825 0.52 +1.5
Or see this region's nucleotide sequence