Experiment: CDM1 without Leucine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15865 and BBR_RS15870 are separated by 135 nucleotides BBR_RS15870 and BBR_RS20940 are separated by 28 nucleotides BBR_RS20940 and BBR_RS20945 overlap by 59 nucleotides BBR_RS20945 and BBR_RS15875 are separated by 31 nucleotides BBR_RS15875 and BBR_RS15880 are separated by 162 nucleotides
BBR_RS15865: BBR_RS15865 - GNAT family N-acetyltransferase, at 1,323,254 to 1,323,787
_RS15865
BBR_RS15870: BBR_RS15870 - hypothetical protein, at 1,323,923 to 1,324,252
_RS15870
BBR_RS20940: BBR_RS20940 - hypothetical protein, at 1,324,281 to 1,324,487
_RS20940
BBR_RS20945: BBR_RS20945 - hypothetical protein, at 1,324,429 to 1,324,630
_RS20945
BBR_RS15875: BBR_RS15875 - DNA-binding protein, at 1,324,662 to 1,325,468
_RS15875
BBR_RS15880: BBR_RS15880 - hypothetical protein, at 1,325,631 to 1,325,885
_RS15880
Position (kb)
1324
1325
1326 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1323.739 kb on + strand at 1323.771 kb on + strand at 1323.806 kb on + strand at 1323.814 kb on - strand at 1323.831 kb on - strand at 1323.852 kb on + strand at 1323.860 kb on - strand at 1323.881 kb on - strand at 1323.911 kb on + strand at 1323.919 kb on - strand at 1323.967 kb on - strand, within BBR_RS15870 at 1323.979 kb on - strand, within BBR_RS15870 at 1323.989 kb on + strand, within BBR_RS15870 at 1323.997 kb on - strand, within BBR_RS15870 at 1323.997 kb on - strand, within BBR_RS15870 at 1324.029 kb on + strand, within BBR_RS15870 at 1324.159 kb on + strand, within BBR_RS15870 at 1324.205 kb on - strand, within BBR_RS15870 at 1324.247 kb on + strand at 1324.255 kb on - strand at 1324.269 kb on + strand at 1324.762 kb on + strand, within BBR_RS15875 at 1324.764 kb on + strand, within BBR_RS15875 at 1324.767 kb on + strand, within BBR_RS15875 at 1324.902 kb on + strand, within BBR_RS15875 at 1324.926 kb on + strand, within BBR_RS15875 at 1324.927 kb on - strand, within BBR_RS15875 at 1324.933 kb on - strand, within BBR_RS15875 at 1324.959 kb on - strand, within BBR_RS15875 at 1324.998 kb on + strand, within BBR_RS15875 at 1324.998 kb on + strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.011 kb on - strand, within BBR_RS15875 at 1325.011 kb on - strand, within BBR_RS15875 at 1325.026 kb on + strand, within BBR_RS15875 at 1325.035 kb on + strand, within BBR_RS15875 at 1325.081 kb on - strand, within BBR_RS15875 at 1325.116 kb on - strand, within BBR_RS15875 at 1325.132 kb on - strand, within BBR_RS15875 at 1325.202 kb on - strand, within BBR_RS15875 at 1325.232 kb on + strand, within BBR_RS15875 at 1325.303 kb on + strand, within BBR_RS15875 at 1325.389 kb on + strand at 1325.395 kb on + strand at 1325.418 kb on - strand at 1325.579 kb on + strand at 1325.634 kb on + strand at 1325.720 kb on - strand, within BBR_RS15880 at 1325.737 kb on + strand, within BBR_RS15880 at 1325.791 kb on + strand, within BBR_RS15880 at 1325.804 kb on - strand, within BBR_RS15880 at 1325.823 kb on + strand, within BBR_RS15880 at 1325.998 kb on + strand at 1326.000 kb on - strand at 1326.000 kb on - strand at 1326.021 kb on - strand at 1326.034 kb on + strand at 1326.132 kb on + strand at 1326.172 kb on - strand at 1326.172 kb on - strand at 1326.204 kb on - strand at 1326.412 kb on + strand at 1326.435 kb on + strand at 1326.459 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Leucine remove 1,323,739 + -2.6 1,323,771 + -1.5 1,323,806 + -0.8 1,323,814 - -0.5 1,323,831 - +0.0 1,323,852 + -1.0 1,323,860 - -0.6 1,323,881 - +0.1 1,323,911 + -1.5 1,323,919 - -0.5 1,323,967 - BBR_RS15870 0.13 +1.7 1,323,979 - BBR_RS15870 0.17 -3.9 1,323,989 + BBR_RS15870 0.20 -2.0 1,323,997 - BBR_RS15870 0.22 -2.0 1,323,997 - BBR_RS15870 0.22 -1.8 1,324,029 + BBR_RS15870 0.32 -0.9 1,324,159 + BBR_RS15870 0.72 -1.8 1,324,205 - BBR_RS15870 0.85 +1.3 1,324,247 + +1.1 1,324,255 - -1.5 1,324,269 + -1.3 1,324,762 + BBR_RS15875 0.12 -1.7 1,324,764 + BBR_RS15875 0.13 -1.3 1,324,767 + BBR_RS15875 0.13 -1.7 1,324,902 + BBR_RS15875 0.30 -0.7 1,324,926 + BBR_RS15875 0.33 -0.2 1,324,927 - BBR_RS15875 0.33 -1.3 1,324,933 - BBR_RS15875 0.34 -0.7 1,324,959 - BBR_RS15875 0.37 -2.7 1,324,998 + BBR_RS15875 0.42 -2.7 1,324,998 + BBR_RS15875 0.42 +0.3 1,325,006 - BBR_RS15875 0.43 -2.4 1,325,006 - BBR_RS15875 0.43 -0.5 1,325,006 - BBR_RS15875 0.43 -3.0 1,325,006 - BBR_RS15875 0.43 +0.6 1,325,006 - BBR_RS15875 0.43 -1.9 1,325,006 - BBR_RS15875 0.43 -0.2 1,325,011 - BBR_RS15875 0.43 +1.9 1,325,011 - BBR_RS15875 0.43 -0.6 1,325,026 + BBR_RS15875 0.45 +0.4 1,325,035 + BBR_RS15875 0.46 -0.8 1,325,081 - BBR_RS15875 0.52 +0.1 1,325,116 - BBR_RS15875 0.56 -2.4 1,325,132 - BBR_RS15875 0.58 -0.9 1,325,202 - BBR_RS15875 0.67 -0.4 1,325,232 + BBR_RS15875 0.71 +0.1 1,325,303 + BBR_RS15875 0.79 -1.1 1,325,389 + -1.4 1,325,395 + -2.1 1,325,418 - -0.1 1,325,579 + -1.1 1,325,634 + -2.0 1,325,720 - BBR_RS15880 0.35 -1.3 1,325,737 + BBR_RS15880 0.42 -2.7 1,325,791 + BBR_RS15880 0.63 +0.0 1,325,804 - BBR_RS15880 0.68 -1.1 1,325,823 + BBR_RS15880 0.75 -1.2 1,325,998 + -0.6 1,326,000 - -1.3 1,326,000 - -1.3 1,326,021 - -0.8 1,326,034 + +0.9 1,326,132 + -1.8 1,326,172 - +1.1 1,326,172 - -1.4 1,326,204 - +1.5 1,326,412 + -0.3 1,326,435 + -1.2 1,326,459 - -2.8
Or see this region's nucleotide sequence