Experiment: nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22015 and HSERO_RS22020 are separated by 86 nucleotides HSERO_RS22020 and HSERO_RS22025 are separated by 19 nucleotides HSERO_RS22025 and HSERO_RS22030 are separated by 477 nucleotides
HSERO_RS22015: HSERO_RS22015 - DNA-binding protein, at 5,033,820 to 5,034,212
_RS22015
HSERO_RS22020: HSERO_RS22020 - membrane protein, at 5,034,299 to 5,034,904
_RS22020
HSERO_RS22025: HSERO_RS22025 - aromatic amino acid aminotransferase, at 5,034,924 to 5,036,114
_RS22025
HSERO_RS22030: HSERO_RS22030 - LysR family transcriptional regulator, at 5,036,592 to 5,037,515
_RS22030
Position (kb) 5034
5035
5036
5037 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5033.980 kb on + strand, within HSERO_RS22015 at 5034.099 kb on - strand, within HSERO_RS22015 at 5034.151 kb on - strand, within HSERO_RS22015 at 5034.167 kb on + strand, within HSERO_RS22015 at 5034.195 kb on + strand at 5034.236 kb on + strand at 5034.337 kb on - strand at 5034.385 kb on - strand, within HSERO_RS22020 at 5034.460 kb on + strand, within HSERO_RS22020 at 5034.509 kb on + strand, within HSERO_RS22020 at 5034.573 kb on + strand, within HSERO_RS22020 at 5034.578 kb on - strand, within HSERO_RS22020 at 5034.810 kb on + strand, within HSERO_RS22020 at 5035.064 kb on + strand, within HSERO_RS22025 at 5035.272 kb on - strand, within HSERO_RS22025 at 5035.441 kb on + strand, within HSERO_RS22025 at 5035.606 kb on + strand, within HSERO_RS22025 at 5035.662 kb on - strand, within HSERO_RS22025 at 5036.103 kb on - strand at 5036.214 kb on + strand at 5036.222 kb on - strand at 5036.245 kb on + strand at 5036.389 kb on + strand at 5036.399 kb on - strand at 5036.461 kb on + strand at 5036.507 kb on + strand at 5036.512 kb on + strand at 5036.605 kb on - strand at 5036.614 kb on + strand at 5036.622 kb on - strand at 5036.661 kb on + strand at 5036.661 kb on + strand at 5036.705 kb on + strand, within HSERO_RS22030 at 5036.752 kb on + strand, within HSERO_RS22030 at 5036.805 kb on + strand, within HSERO_RS22030 at 5036.820 kb on - strand, within HSERO_RS22030 at 5036.834 kb on + strand, within HSERO_RS22030 at 5036.871 kb on + strand, within HSERO_RS22030 at 5037.036 kb on - strand, within HSERO_RS22030 at 5037.036 kb on - strand, within HSERO_RS22030
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 20 mM remove 5,033,980 + HSERO_RS22015 0.41 +1.8 5,034,099 - HSERO_RS22015 0.71 +0.4 5,034,151 - HSERO_RS22015 0.84 +1.0 5,034,167 + HSERO_RS22015 0.88 +0.2 5,034,195 + +1.1 5,034,236 + -0.3 5,034,337 - +2.2 5,034,385 - HSERO_RS22020 0.14 +1.6 5,034,460 + HSERO_RS22020 0.27 -2.2 5,034,509 + HSERO_RS22020 0.35 +4.3 5,034,573 + HSERO_RS22020 0.45 +1.1 5,034,578 - HSERO_RS22020 0.46 -1.9 5,034,810 + HSERO_RS22020 0.84 -0.0 5,035,064 + HSERO_RS22025 0.12 -1.1 5,035,272 - HSERO_RS22025 0.29 +0.3 5,035,441 + HSERO_RS22025 0.43 -0.5 5,035,606 + HSERO_RS22025 0.57 -0.6 5,035,662 - HSERO_RS22025 0.62 -0.6 5,036,103 - -0.4 5,036,214 + +0.3 5,036,222 - -1.0 5,036,245 + -0.5 5,036,389 + +0.1 5,036,399 - +2.6 5,036,461 + -0.2 5,036,507 + -3.8 5,036,512 + +0.9 5,036,605 - +0.1 5,036,614 + -0.2 5,036,622 - -0.6 5,036,661 + +1.2 5,036,661 + -0.3 5,036,705 + HSERO_RS22030 0.12 -0.5 5,036,752 + HSERO_RS22030 0.17 -0.5 5,036,805 + HSERO_RS22030 0.23 -0.1 5,036,820 - HSERO_RS22030 0.25 -0.6 5,036,834 + HSERO_RS22030 0.26 -0.1 5,036,871 + HSERO_RS22030 0.30 -0.2 5,037,036 - HSERO_RS22030 0.48 +0.4 5,037,036 - HSERO_RS22030 0.48 -1.1
Or see this region's nucleotide sequence