Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_30095 and AO356_30100 overlap by 4 nucleotides AO356_30100 and AO356_30105 overlap by 19 nucleotides AO356_30105 and AO356_30110 are separated by 13 nucleotides AO356_30110 and AO356_30115 overlap by 4 nucleotides
AO356_30095: AO356_30095 - formyltetrahydrofolate deformylase, at 7,023,925 to 7,024,791
_30095
AO356_30100: AO356_30100 - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase, at 7,024,788 to 7,025,693
_30100
AO356_30105: AO356_30105 - sarcosine oxidase subunit beta, at 7,025,675 to 7,026,916
_30105
AO356_30110: AO356_30110 - sarcosine oxidase subunit delta, at 7,026,930 to 7,027,232
_30110
AO356_30115: AO356_30115 - aminomethyltransferase, at 7,027,229 to 7,030,126
_30115
Position (kb) 7025
7026
7027 Strain fitness (log2 ratio) -1
0
1
2
3 at 7024.935 kb on + strand at 7024.935 kb on + strand, within AO356_30100 at 7024.935 kb on + strand, within AO356_30100 at 7024.935 kb on + strand, within AO356_30100 at 7024.935 kb on + strand, within AO356_30100 at 7024.936 kb on - strand, within AO356_30100 at 7025.147 kb on - strand, within AO356_30100 at 7025.147 kb on - strand, within AO356_30100 at 7025.229 kb on + strand, within AO356_30100 at 7025.258 kb on - strand, within AO356_30100 at 7025.674 kb on + strand at 7025.688 kb on + strand at 7025.762 kb on + strand at 7025.763 kb on - strand at 7025.763 kb on - strand at 7025.770 kb on - strand at 7025.770 kb on - strand at 7025.770 kb on - strand at 7025.770 kb on - strand at 7025.770 kb on - strand at 7025.811 kb on - strand, within AO356_30105 at 7025.811 kb on - strand, within AO356_30105 at 7025.826 kb on - strand, within AO356_30105 at 7025.826 kb on - strand, within AO356_30105 at 7025.839 kb on + strand, within AO356_30105 at 7025.840 kb on - strand, within AO356_30105 at 7025.840 kb on - strand, within AO356_30105 at 7025.864 kb on + strand, within AO356_30105 at 7025.864 kb on + strand, within AO356_30105 at 7025.916 kb on - strand, within AO356_30105 at 7025.942 kb on + strand, within AO356_30105 at 7025.943 kb on - strand, within AO356_30105 at 7026.002 kb on + strand, within AO356_30105 at 7026.003 kb on - strand, within AO356_30105 at 7026.509 kb on + strand, within AO356_30105 at 7026.510 kb on - strand, within AO356_30105 at 7026.757 kb on + strand, within AO356_30105 at 7026.985 kb on - strand, within AO356_30110 at 7026.985 kb on - strand, within AO356_30110 at 7026.985 kb on - strand, within AO356_30110 at 7027.048 kb on - strand, within AO356_30110 at 7027.051 kb on + strand, within AO356_30110 at 7027.052 kb on - strand, within AO356_30110 at 7027.052 kb on - strand, within AO356_30110 at 7027.052 kb on - strand, within AO356_30110 at 7027.052 kb on - strand, within AO356_30110 at 7027.114 kb on - strand, within AO356_30110 at 7027.203 kb on - strand at 7027.385 kb on + strand at 7027.386 kb on - strand at 7027.386 kb on - strand at 7027.386 kb on - strand at 7027.589 kb on + strand, within AO356_30115 at 7027.589 kb on + strand, within AO356_30115 at 7027.590 kb on - strand, within AO356_30115 at 7027.592 kb on + strand, within AO356_30115 at 7027.604 kb on + strand, within AO356_30115 at 7027.604 kb on + strand, within AO356_30115 at 7027.605 kb on - strand, within AO356_30115 at 7027.709 kb on + strand, within AO356_30115 at 7027.709 kb on + strand, within AO356_30115 at 7027.838 kb on + strand, within AO356_30115 at 7027.838 kb on + strand, within AO356_30115 at 7027.839 kb on - strand, within AO356_30115
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 7,024,935 + +3.2 7,024,935 + AO356_30100 0.16 -1.5 7,024,935 + AO356_30100 0.16 +0.3 7,024,935 + AO356_30100 0.16 -0.3 7,024,935 + AO356_30100 0.16 +0.3 7,024,936 - AO356_30100 0.16 +0.3 7,025,147 - AO356_30100 0.40 +0.1 7,025,147 - AO356_30100 0.40 -0.1 7,025,229 + AO356_30100 0.49 -1.0 7,025,258 - AO356_30100 0.52 -0.1 7,025,674 + -0.5 7,025,688 + +0.7 7,025,762 + +1.1 7,025,763 - +0.3 7,025,763 - +0.2 7,025,770 - -0.6 7,025,770 - +1.0 7,025,770 - +0.5 7,025,770 - +0.1 7,025,770 - +2.3 7,025,811 - AO356_30105 0.11 -0.9 7,025,811 - AO356_30105 0.11 -0.3 7,025,826 - AO356_30105 0.12 +0.2 7,025,826 - AO356_30105 0.12 +1.2 7,025,839 + AO356_30105 0.13 -0.1 7,025,840 - AO356_30105 0.13 +0.0 7,025,840 - AO356_30105 0.13 +0.5 7,025,864 + AO356_30105 0.15 +0.0 7,025,864 + AO356_30105 0.15 +0.4 7,025,916 - AO356_30105 0.19 +0.2 7,025,942 + AO356_30105 0.21 +0.4 7,025,943 - AO356_30105 0.22 -0.1 7,026,002 + AO356_30105 0.26 +0.0 7,026,003 - AO356_30105 0.26 +0.2 7,026,509 + AO356_30105 0.67 -0.7 7,026,510 - AO356_30105 0.67 -0.1 7,026,757 + AO356_30105 0.87 +0.6 7,026,985 - AO356_30110 0.18 -0.4 7,026,985 - AO356_30110 0.18 -0.2 7,026,985 - AO356_30110 0.18 -0.5 7,027,048 - AO356_30110 0.39 -0.7 7,027,051 + AO356_30110 0.40 -0.5 7,027,052 - AO356_30110 0.40 -0.5 7,027,052 - AO356_30110 0.40 +0.0 7,027,052 - AO356_30110 0.40 +0.3 7,027,052 - AO356_30110 0.40 -1.1 7,027,114 - AO356_30110 0.61 -0.1 7,027,203 - -0.8 7,027,385 + +0.9 7,027,386 - -0.9 7,027,386 - +1.0 7,027,386 - -0.4 7,027,589 + AO356_30115 0.12 -0.3 7,027,589 + AO356_30115 0.12 +0.4 7,027,590 - AO356_30115 0.12 +0.8 7,027,592 + AO356_30115 0.13 -1.2 7,027,604 + AO356_30115 0.13 -0.8 7,027,604 + AO356_30115 0.13 -0.5 7,027,605 - AO356_30115 0.13 +0.1 7,027,709 + AO356_30115 0.17 +0.2 7,027,709 + AO356_30115 0.17 +0.6 7,027,838 + AO356_30115 0.21 +0.6 7,027,838 + AO356_30115 0.21 +0.1 7,027,839 - AO356_30115 0.21 -0.7
Or see this region's nucleotide sequence