Experiment: LB, 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00425 and SMc00461 overlap by 8 nucleotides SMc00461 and SMc00427 are separated by 98 nucleotides SMc00427 and SMc00428 are separated by 16 nucleotides SMc00428 and SMc00429 are separated by 84 nucleotides
SMc00425: SMc00425 - transcriptional regulator, at 373,932 to 374,369
SMc00425
SMc00461: SMc00461 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 374,362 to 374,844
SMc00461
SMc00427: SMc00427 - peptide chain release factor RF-3 protein, at 374,943 to 376,526
SMc00427
SMc00428: SMc00428 - transmembrane protein, at 376,543 to 377,439
SMc00428
SMc00429: SMc00429 - transcriptional regulator, at 377,524 to 378,333
SMc00429
Position (kb) 374
375
376
377 Strain fitness (log2 ratio) -1
0
1 at 374.165 kb on + strand, within SMc00425 at 374.165 kb on + strand, within SMc00425 at 374.165 kb on + strand, within SMc00425 at 374.363 kb on + strand at 374.364 kb on - strand at 374.368 kb on - strand at 374.368 kb on - strand at 374.368 kb on - strand at 374.652 kb on + strand, within SMc00461 at 374.653 kb on - strand, within SMc00461 at 374.858 kb on + strand at 374.859 kb on - strand at 374.859 kb on - strand at 374.909 kb on + strand at 375.238 kb on - strand, within SMc00427 at 375.396 kb on + strand, within SMc00427 at 375.487 kb on - strand, within SMc00427 at 375.487 kb on - strand, within SMc00427 at 376.287 kb on + strand, within SMc00427 at 376.504 kb on - strand at 376.544 kb on + strand at 376.545 kb on - strand at 376.545 kb on - strand at 377.194 kb on - strand, within SMc00428 at 377.417 kb on + strand at 377.418 kb on - strand at 377.418 kb on - strand at 377.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, 30C remove 374,165 + SMc00425 0.53 +0.6 374,165 + SMc00425 0.53 +0.7 374,165 + SMc00425 0.53 +0.1 374,363 + +0.8 374,364 - +0.4 374,368 - -0.5 374,368 - -0.0 374,368 - +1.2 374,652 + SMc00461 0.60 -1.3 374,653 - SMc00461 0.60 -0.1 374,858 + -0.9 374,859 - +0.3 374,859 - -0.3 374,909 + -0.2 375,238 - SMc00427 0.19 +1.1 375,396 + SMc00427 0.29 -0.8 375,487 - SMc00427 0.34 -1.1 375,487 - SMc00427 0.34 -0.3 376,287 + SMc00427 0.85 -0.9 376,504 - -0.9 376,544 + +0.2 376,545 - -0.0 376,545 - -0.8 377,194 - SMc00428 0.73 +0.0 377,417 + -0.2 377,418 - +1.6 377,418 - -0.3 377,501 + -0.2
Or see this region's nucleotide sequence