Experiment: LB, 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20304 and SM_b20306 overlap by 357 nucleotides SM_b20306 and SM_b20305 overlap by 73 nucleotides SM_b20305 and SM_b20307 are separated by 92 nucleotides SM_b20307 and SM_b20312 are separated by 78 nucleotides
SM_b20304: SM_b20304 - ISRm2011-2 transposase, C-terminal portion, at 310,762 to 311,358
_b20304
SM_b20306: SM_b20306 - TRm2011-2 protein, at 311,002 to 311,373
_b20306
SM_b20305: SM_b20305 - ISRm2011-2 transposase, N-terminal portion, at 311,301 to 311,708
_b20305
SM_b20307: SM_b20307 - dihydroxyacetone kinase, at 311,801 to 312,448
_b20307
SM_b20312: SM_b20312 - dihydroxyacetone kinase, at 312,527 to 314,614
_b20312
Position (kb) 311
312
313 Strain fitness (log2 ratio) -2
-1
0
1
2 at 311.933 kb on - strand, within SM_b20307 at 312.528 kb on + strand at 312.639 kb on + strand at 312.713 kb on - strand at 312.714 kb on + strand at 312.714 kb on + strand at 312.714 kb on + strand at 312.715 kb on - strand at 312.715 kb on - strand at 312.715 kb on - strand at 312.715 kb on - strand at 312.967 kb on + strand, within SM_b20312 at 312.967 kb on + strand, within SM_b20312 at 312.969 kb on + strand, within SM_b20312 at 312.970 kb on - strand, within SM_b20312 at 313.143 kb on - strand, within SM_b20312 at 313.143 kb on - strand, within SM_b20312 at 313.336 kb on - strand, within SM_b20312 at 313.336 kb on - strand, within SM_b20312
Per-strain Table
Position Strand Gene LocusTag Fraction LB, 30C remove 311,933 - SM_b20307 0.20 -1.7 312,528 + -0.4 312,639 + -0.5 312,713 - -0.5 312,714 + +0.5 312,714 + -0.1 312,714 + +1.8 312,715 - +0.1 312,715 - -0.9 312,715 - -0.2 312,715 - -0.6 312,967 + SM_b20312 0.21 -0.7 312,967 + SM_b20312 0.21 -0.1 312,969 + SM_b20312 0.21 -0.2 312,970 - SM_b20312 0.21 -0.6 313,143 - SM_b20312 0.30 -0.4 313,143 - SM_b20312 0.30 -2.1 313,336 - SM_b20312 0.39 -2.1 313,336 - SM_b20312 0.39 +0.9
Or see this region's nucleotide sequence