Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08800 and MMJJ_RS08805 are separated by 1 nucleotides MMJJ_RS08805 and MMJJ_RS08810 are separated by 67 nucleotides MMJJ_RS08810 and MMJJ_RS08815 are separated by 9 nucleotides MMJJ_RS08815 and MMJJ_RS08820 are separated by 199 nucleotides
MMJJ_RS08800: MMJJ_RS08800 - sugar phosphate nucleotidyltransferase, at 1,621,191 to 1,622,426
_RS08800
MMJJ_RS08805: MMJJ_RS08805 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 1,622,428 to 1,622,898
_RS08805
MMJJ_RS08810: MMJJ_RS08810 - DUF4040 domain-containing protein, at 1,622,966 to 1,623,208
_RS08810
MMJJ_RS08815: MMJJ_RS08815 - cation:proton antiporter, at 1,623,218 to 1,623,472
_RS08815
MMJJ_RS08820: MMJJ_RS08820 - pyridoxal phosphate-dependent aminotransferase, at 1,623,672 to 1,624,799
_RS08820
Position (kb)
1622
1623
1624 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1622.507 kb on - strand, within MMJJ_RS08805 at 1622.526 kb on - strand, within MMJJ_RS08805 at 1622.527 kb on + strand, within MMJJ_RS08805 at 1622.592 kb on + strand, within MMJJ_RS08805 at 1622.639 kb on - strand, within MMJJ_RS08805 at 1622.641 kb on - strand, within MMJJ_RS08805 at 1622.642 kb on + strand, within MMJJ_RS08805 at 1622.649 kb on + strand, within MMJJ_RS08805 at 1622.779 kb on - strand, within MMJJ_RS08805 at 1622.780 kb on + strand, within MMJJ_RS08805 at 1622.784 kb on + strand, within MMJJ_RS08805 at 1622.784 kb on + strand, within MMJJ_RS08805 at 1622.784 kb on + strand, within MMJJ_RS08805 at 1622.792 kb on - strand, within MMJJ_RS08805 at 1622.793 kb on + strand, within MMJJ_RS08805 at 1622.813 kb on + strand at 1622.814 kb on - strand, within MMJJ_RS08805 at 1622.814 kb on - strand, within MMJJ_RS08805 at 1622.814 kb on - strand, within MMJJ_RS08805 at 1622.814 kb on - strand, within MMJJ_RS08805 at 1622.815 kb on + strand, within MMJJ_RS08805 at 1622.872 kb on - strand at 1622.872 kb on - strand at 1622.882 kb on - strand at 1622.882 kb on - strand at 1622.882 kb on - strand at 1622.883 kb on + strand at 1622.883 kb on + strand at 1622.884 kb on - strand at 1622.955 kb on - strand at 1622.967 kb on - strand at 1622.992 kb on - strand, within MMJJ_RS08810 at 1623.363 kb on + strand, within MMJJ_RS08815 at 1623.364 kb on - strand, within MMJJ_RS08815 at 1623.364 kb on - strand at 1623.452 kb on - strand at 1623.452 kb on - strand at 1623.485 kb on - strand at 1623.509 kb on + strand at 1623.524 kb on - strand at 1623.524 kb on - strand at 1623.524 kb on - strand at 1623.524 kb on - strand at 1623.531 kb on + strand at 1623.538 kb on + strand at 1623.538 kb on + strand at 1623.544 kb on - strand at 1623.554 kb on - strand at 1623.559 kb on - strand at 1623.559 kb on - strand at 1623.576 kb on - strand at 1623.581 kb on - strand at 1623.581 kb on - strand at 1623.581 kb on - strand at 1623.581 kb on - strand at 1623.585 kb on - strand at 1623.585 kb on - strand at 1623.587 kb on - strand at 1623.587 kb on - strand at 1623.587 kb on - strand at 1623.587 kb on - strand at 1623.588 kb on + strand at 1623.588 kb on + strand at 1623.589 kb on - strand at 1623.589 kb on - strand at 1623.590 kb on + strand at 1623.590 kb on + strand at 1623.590 kb on + strand at 1623.608 kb on + strand at 1623.646 kb on - strand at 1623.647 kb on + strand at 1623.647 kb on + strand at 1623.647 kb on + strand at 1623.648 kb on - strand at 1623.648 kb on - strand at 1623.648 kb on - strand at 1623.648 kb on - strand at 1623.648 kb on - strand at 1623.648 kb on - strand at 1623.648 kb on - strand at 1623.649 kb on + strand at 1623.649 kb on + strand at 1623.649 kb on + strand at 1623.649 kb on + strand at 1623.650 kb on - strand at 1623.650 kb on - strand at 1623.650 kb on - strand at 1623.651 kb on + strand at 1623.651 kb on + strand at 1623.651 kb on + strand at 1623.651 kb on + strand at 1623.651 kb on + strand at 1623.653 kb on + strand at 1623.653 kb on + strand at 1623.653 kb on + strand at 1623.656 kb on - strand at 1623.656 kb on - strand at 1623.660 kb on - strand at 1623.660 kb on - strand at 1623.722 kb on + strand at 1623.725 kb on - strand at 1623.796 kb on - strand, within MMJJ_RS08820 at 1623.837 kb on - strand, within MMJJ_RS08820 at 1623.837 kb on - strand, within MMJJ_RS08820 at 1623.838 kb on + strand, within MMJJ_RS08820 at 1623.907 kb on - strand, within MMJJ_RS08820 at 1623.907 kb on - strand, within MMJJ_RS08820 at 1623.907 kb on - strand, within MMJJ_RS08820 at 1623.907 kb on - strand, within MMJJ_RS08820 at 1623.975 kb on + strand, within MMJJ_RS08820
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 1,622,507 - MMJJ_RS08805 0.17 +0.4 1,622,526 - MMJJ_RS08805 0.21 -1.4 1,622,527 + MMJJ_RS08805 0.21 +0.7 1,622,592 + MMJJ_RS08805 0.35 +0.0 1,622,639 - MMJJ_RS08805 0.45 +0.1 1,622,641 - MMJJ_RS08805 0.45 +0.5 1,622,642 + MMJJ_RS08805 0.45 +0.2 1,622,649 + MMJJ_RS08805 0.47 +0.3 1,622,779 - MMJJ_RS08805 0.75 -0.1 1,622,780 + MMJJ_RS08805 0.75 +1.0 1,622,784 + MMJJ_RS08805 0.76 +0.1 1,622,784 + MMJJ_RS08805 0.76 +0.0 1,622,784 + MMJJ_RS08805 0.76 -1.5 1,622,792 - MMJJ_RS08805 0.77 -0.4 1,622,793 + MMJJ_RS08805 0.77 -0.5 1,622,813 + -1.0 1,622,814 - MMJJ_RS08805 0.82 -0.9 1,622,814 - MMJJ_RS08805 0.82 -0.2 1,622,814 - MMJJ_RS08805 0.82 +0.7 1,622,814 - MMJJ_RS08805 0.82 +0.2 1,622,815 + MMJJ_RS08805 0.82 -0.1 1,622,872 - +0.7 1,622,872 - +0.3 1,622,882 - +0.4 1,622,882 - +0.5 1,622,882 - +1.9 1,622,883 + +0.3 1,622,883 + +0.4 1,622,884 - +0.3 1,622,955 - +0.3 1,622,967 - +0.7 1,622,992 - MMJJ_RS08810 0.11 -1.2 1,623,363 + MMJJ_RS08815 0.57 -2.0 1,623,364 - MMJJ_RS08815 0.57 +0.3 1,623,364 - +1.3 1,623,452 - -0.7 1,623,452 - -0.7 1,623,485 - -2.8 1,623,509 + +0.3 1,623,524 - -2.0 1,623,524 - -3.6 1,623,524 - -2.9 1,623,524 - -1.2 1,623,531 + +0.8 1,623,538 + -2.8 1,623,538 + -3.8 1,623,544 - -1.8 1,623,554 - -2.1 1,623,559 - -1.2 1,623,559 - -4.2 1,623,576 - +0.0 1,623,581 - -2.9 1,623,581 - -2.8 1,623,581 - -1.8 1,623,581 - -3.1 1,623,585 - -3.2 1,623,585 - -1.6 1,623,587 - -2.4 1,623,587 - -1.2 1,623,587 - -3.4 1,623,587 - -1.7 1,623,588 + -1.2 1,623,588 + -2.5 1,623,589 - -3.1 1,623,589 - -3.8 1,623,590 + -2.9 1,623,590 + -1.5 1,623,590 + -2.5 1,623,608 + +1.3 1,623,646 - -1.8 1,623,647 + +0.0 1,623,647 + -2.8 1,623,647 + -1.0 1,623,648 - -3.5 1,623,648 - -1.7 1,623,648 - -0.2 1,623,648 - -3.8 1,623,648 - -2.5 1,623,648 - -3.1 1,623,648 - -1.7 1,623,649 + -3.0 1,623,649 + -0.2 1,623,649 + -1.0 1,623,649 + -2.0 1,623,650 - -1.9 1,623,650 - -3.5 1,623,650 - -1.5 1,623,651 + -1.0 1,623,651 + -3.4 1,623,651 + -1.1 1,623,651 + -2.2 1,623,651 + -4.0 1,623,653 + -0.9 1,623,653 + +1.3 1,623,653 + +0.2 1,623,656 - -0.2 1,623,656 - +0.9 1,623,660 - -0.4 1,623,660 - -0.1 1,623,722 + +0.9 1,623,725 - +0.2 1,623,796 - MMJJ_RS08820 0.11 -1.7 1,623,837 - MMJJ_RS08820 0.15 -0.1 1,623,837 - MMJJ_RS08820 0.15 +0.5 1,623,838 + MMJJ_RS08820 0.15 -0.4 1,623,907 - MMJJ_RS08820 0.21 -1.4 1,623,907 - MMJJ_RS08820 0.21 +0.7 1,623,907 - MMJJ_RS08820 0.21 -0.4 1,623,907 - MMJJ_RS08820 0.21 -0.1 1,623,975 + MMJJ_RS08820 0.27 +0.0
Or see this region's nucleotide sequence